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The map label for this gene is mscL

Identifier: 28210182

GI number: 28210182

Start: 427480

End: 427881

Strand: Direct

Name: mscL

Synonym: CTC00429

Alternate gene names: 28210182

Gene position: 427480-427881 (Clockwise)

Preceding gene: 28210181

Following gene: 28210183

Centisome position: 15.27

GC content: 26.12

Gene sequence:

>402_bases
ATGTTTAAAGATGTAGTAGAGGAATTTAAAAAGTTTGCCATAAAAGGAAATGCAATTGATTTGGCAGTAGGTGTAGTTAT
AGGGGGAGCTTTTGGTAAGATAGTAACTTCTTTGGTACAAGATATAATAATGCCTGCAGTAGGATTATTGTTGGGAAAAG
TTAATTTTAAAACATTAAGTTTAACAGTAACATCTATAGATGGTTCTGAAATAGTATTAAAATATGGACAATTTATTCAA
TCAGTAGTTGATTTTATAATAATATCTTTTTCTATATTCTTATTTGTAAAACTTATAAATTCTTTTAAGAAAAAAGAAGA
AGAGAAGCCTAAAGTAGAAGAACCATCTAAGGAAGAAAAATTACTTGCTGAAATAAGAGATATTTTAAAAGAAAGTAAAT
AA

Upstream 100 bases:

>100_bases
ACAGAACATAAGATAATAGAAGGCATTTTAAGAGAAATAGGAAAAGAGAAAGATATAGAATTAATTTAAATATATTTTAT
TAGAACAAAGGAGGAATACA

Downstream 100 bases:

>100_bases
ATTAAAAGCTTTTAGAAACTATTTTAAATAGTTTCTTTTTTTATGTTTTTATGTTTTTATGTATTGACAAAGTTGGAAAT
AAGTAATAATATAATATATG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MFKDVVEEFKKFAIKGNAIDLAVGVVIGGAFGKIVTSLVQDIIMPAVGLLLGKVNFKTLSLTVTSIDGSEIVLKYGQFIQ
SVVDFIIISFSIFLFVKLINSFKKKEEEKPKVEEPSKEEKLLAEIRDILKESK

Sequences:

>Translated_133_residues
MFKDVVEEFKKFAIKGNAIDLAVGVVIGGAFGKIVTSLVQDIIMPAVGLLLGKVNFKTLSLTVTSIDGSEIVLKYGQFIQ
SVVDFIIISFSIFLFVKLINSFKKKEEEKPKVEEPSKEEKLLAEIRDILKESK
>Mature_133_residues
MFKDVVEEFKKFAIKGNAIDLAVGVVIGGAFGKIVTSLVQDIIMPAVGLLLGKVNFKTLSLTVTSIDGSEIVLKYGQFIQ
SVVDFIIISFSIFLFVKLINSFKKKEEEKPKVEEPSKEEKLLAEIRDILKESK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=131, Percent_Identity=52.6717557251908, Blast_Score=147, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CLOTE (Q898L6)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781126.1
- ProteinModelPortal:   Q898L6
- SMR:   Q898L6
- GeneID:   1060031
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00429
- NMPDR:   fig|212717.1.peg.348
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q898L6
- ProtClustDB:   PRK00567
- BioCyc:   CTET212717:CTC_00429-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14768; Mature: 14768

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x406f63dc)-; HASH(0xcd43b74)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKDVVEEFKKFAIKGNAIDLAVGVVIGGAFGKIVTSLVQDIIMPAVGLLLGKVNFKTLS
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LTVTSIDGSEIVLKYGQFIQSVVDFIIISFSIFLFVKLINSFKKKEEEKPKVEEPSKEEK
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LLAEIRDILKESK
HHHHHHHHHHCCH
>Mature Secondary Structure
MFKDVVEEFKKFAIKGNAIDLAVGVVIGGAFGKIVTSLVQDIIMPAVGLLLGKVNFKTLS
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LTVTSIDGSEIVLKYGQFIQSVVDFIIISFSIFLFVKLINSFKKKEEEKPKVEEPSKEEK
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LLAEIRDILKESK
HHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12552129