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The map label for this gene is mepA [H]

Identifier: 28210172

GI number: 28210172

Start: 413931

End: 415319

Strand: Direct

Name: mepA [H]

Synonym: CTC00419

Alternate gene names: 28210172

Gene position: 413931-415319 (Clockwise)

Preceding gene: 28210169

Following gene: 28210173

Centisome position: 14.79

GC content: 31.1

Gene sequence:

>1389_bases
ATGAAAAATACAATATCTACAGCAGAAATGATGGAGAAAGAGAGTATATCAAAGGTGCTTTTAAAATTATCAGTACCTGC
CATAATAGCTATGTTAATTAATGCTATTTATAACATTGTAGATACCATGTTTGTTGGTATGTTAGATAATACCAGTGCTA
TTGCCGCCGTATCTATAGTCTACCCATTATTTATGTTCATTGGAGCTATAGGGGTTATGTTTGGGGCAGGAGGTGCTTCT
TATTTATCAAGACTGTTGGGAGAAAAGAAAAAAGAGGAAGCAGATAAAACCCTAACATCTACTATAATAATGGGATGTAT
ATTCAGTTTGATTTTTACGGTAATTGCCACAGTATTTTTAGATGAATTTTTATTGATGTATGGAGCAACAGAAACCATAA
TGCCCTATGCTAAAGAATACGGATATGTAATAGTATTGGGTTCTATTTTTACTATTGGAACAGCTATTATGAGCAACACT
ATTAGAGCAGAGGGGAATTCAAGGTATAGTATGATAGCTACTTGTGTAGGGGCTGTTGTAAATATAATATTAGATCCCAT
ACTGATGTTTAACTTTAATATGGGTATAAAAGGTGCTGCCATTGCTACGGTTATAGCTCAATTAACATCCTTTATTTTTC
TTTTAAGATATTATTATTCAAATAGAAATTATATAAATTTTAAAATTAATCTTTTTAAACCAACATTTAATATGTTTTTT
GAAATACTTAAGATAGGTATTCCTATTTTTGTAACTCAAGTATTGGCAAGTTTTGCTCTAGGATTTATGAACTTAGGAGC
AAAACCTTATGGTGATGCAGCAGTAGCTGCAATGGGTATAGTATTTAGAGTTATGTCTATAGGATTTTATATAGTGTTTG
GAATAGGACAAGGATTTCAGCCAGTTGCAGGATACAATTATGGAGCTAAAAACTTTACTAGGTTAGAGGAATCTATAAAA
ATATCCATTAGATGGAGTACAATAGCTGCTATAATAACATCTCTTTTATTTATAATTTTTGCAAAAGAATGTATGCTTAT
ATTTACAAGAGATAGAGAAGTTATAGATATAGGTATAAAAGCATTTAGAGCAGCAAGTTTATTATTTCCACTCTTTGGGT
ATGTAAATACTTATGCAGTGCTATTTCAAGCTTTAGGAAAGGCAGTAGGAGCTTTTATATTATCTATTTCTAGACAGGGA
ATATTCTATATTCCTTTAATGTATGTACTGCCAAAATTTATGGGGTTAAATGGTGTAATGTTTTGTCAAACAGCAGCGGA
TGGACTAGCTTTTTTAGAAACTATAATAATGGCCATTTGGCTAAAGAAGCAATTTAAGAAAGAAGTATCTCAAGAATCTA
CATTAGGTGATGTAAAAGAGATTTCATAG

Upstream 100 bases:

>100_bases
ATTTATTTCACAAAGCCTAGGTTTAAAAAATTAAAAAAATAAGACCATGAAGGGAGGACAGTCTAAGCTTTGCTAGGCTT
TCATTAGACAAAAAAAGAAT

Downstream 100 bases:

>100_bases
GTTAAAATCATAAAAATAAGATATACTATAAGGTATAAAATCTAAAGTATATGGGAGAGAAGAAATATGGATTATACATT
AATTGCAACTGCCACTTTTG

Product: Na+ driven multidrug efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKNTISTAEMMEKESISKVLLKLSVPAIIAMLINAIYNIVDTMFVGMLDNTSAIAAVSIVYPLFMFIGAIGVMFGAGGAS
YLSRLLGEKKKEEADKTLTSTIIMGCIFSLIFTVIATVFLDEFLLMYGATETIMPYAKEYGYVIVLGSIFTIGTAIMSNT
IRAEGNSRYSMIATCVGAVVNIILDPILMFNFNMGIKGAAIATVIAQLTSFIFLLRYYYSNRNYINFKINLFKPTFNMFF
EILKIGIPIFVTQVLASFALGFMNLGAKPYGDAAVAAMGIVFRVMSIGFYIVFGIGQGFQPVAGYNYGAKNFTRLEESIK
ISIRWSTIAAIITSLLFIIFAKECMLIFTRDREVIDIGIKAFRAASLLFPLFGYVNTYAVLFQALGKAVGAFILSISRQG
IFYIPLMYVLPKFMGLNGVMFCQTAADGLAFLETIIMAIWLKKQFKKEVSQESTLGDVKEIS

Sequences:

>Translated_462_residues
MKNTISTAEMMEKESISKVLLKLSVPAIIAMLINAIYNIVDTMFVGMLDNTSAIAAVSIVYPLFMFIGAIGVMFGAGGAS
YLSRLLGEKKKEEADKTLTSTIIMGCIFSLIFTVIATVFLDEFLLMYGATETIMPYAKEYGYVIVLGSIFTIGTAIMSNT
IRAEGNSRYSMIATCVGAVVNIILDPILMFNFNMGIKGAAIATVIAQLTSFIFLLRYYYSNRNYINFKINLFKPTFNMFF
EILKIGIPIFVTQVLASFALGFMNLGAKPYGDAAVAAMGIVFRVMSIGFYIVFGIGQGFQPVAGYNYGAKNFTRLEESIK
ISIRWSTIAAIITSLLFIIFAKECMLIFTRDREVIDIGIKAFRAASLLFPLFGYVNTYAVLFQALGKAVGAFILSISRQG
IFYIPLMYVLPKFMGLNGVMFCQTAADGLAFLETIIMAIWLKKQFKKEVSQESTLGDVKEIS
>Mature_462_residues
MKNTISTAEMMEKESISKVLLKLSVPAIIAMLINAIYNIVDTMFVGMLDNTSAIAAVSIVYPLFMFIGAIGVMFGAGGAS
YLSRLLGEKKKEEADKTLTSTIIMGCIFSLIFTVIATVFLDEFLLMYGATETIMPYAKEYGYVIVLGSIFTIGTAIMSNT
IRAEGNSRYSMIATCVGAVVNIILDPILMFNFNMGIKGAAIATVIAQLTSFIFLLRYYYSNRNYINFKINLFKPTFNMFF
EILKIGIPIFVTQVLASFALGFMNLGAKPYGDAAVAAMGIVFRVMSIGFYIVFGIGQGFQPVAGYNYGAKNFTRLEESIK
ISIRWSTIAAIITSLLFIIFAKECMLIFTRDREVIDIGIKAFRAASLLFPLFGYVNTYAVLFQALGKAVGAFILSISRQG
IFYIPLMYVLPKFMGLNGVMFCQTAADGLAFLETIIMAIWLKKQFKKEVSQESTLGDVKEIS

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=337, Percent_Identity=20.1780415430267, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50965; Mature: 50965

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTISTAEMMEKESISKVLLKLSVPAIIAMLINAIYNIVDTMFVGMLDNTSAIAAVSIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
YPLFMFIGAIGVMFGAGGASYLSRLLGEKKKEEADKTLTSTIIMGCIFSLIFTVIATVFL
HHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEFLLMYGATETIMPYAKEYGYVIVLGSIFTIGTAIMSNTIRAEGNSRYSMIATCVGAVV
HHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NIILDPILMFNFNMGIKGAAIATVIAQLTSFIFLLRYYYSNRNYINFKINLFKPTFNMFF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHH
EILKIGIPIFVTQVLASFALGFMNLGAKPYGDAAVAAMGIVFRVMSIGFYIVFGIGQGFQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCC
PVAGYNYGAKNFTRLEESIKISIRWSTIAAIITSLLFIIFAKECMLIFTRDREVIDIGIK
CCCCCCCCCHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHH
AFRAASLLFPLFGYVNTYAVLFQALGKAVGAFILSISRQGIFYIPLMYVLPKFMGLNGVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEE
FCQTAADGLAFLETIIMAIWLKKQFKKEVSQESTLGDVKEIS
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MKNTISTAEMMEKESISKVLLKLSVPAIIAMLINAIYNIVDTMFVGMLDNTSAIAAVSIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
YPLFMFIGAIGVMFGAGGASYLSRLLGEKKKEEADKTLTSTIIMGCIFSLIFTVIATVFL
HHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEFLLMYGATETIMPYAKEYGYVIVLGSIFTIGTAIMSNTIRAEGNSRYSMIATCVGAVV
HHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NIILDPILMFNFNMGIKGAAIATVIAQLTSFIFLLRYYYSNRNYINFKINLFKPTFNMFF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHH
EILKIGIPIFVTQVLASFALGFMNLGAKPYGDAAVAAMGIVFRVMSIGFYIVFGIGQGFQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCC
PVAGYNYGAKNFTRLEESIKISIRWSTIAAIITSLLFIIFAKECMLIFTRDREVIDIGIK
CCCCCCCCCHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHH
AFRAASLLFPLFGYVNTYAVLFQALGKAVGAFILSISRQGIFYIPLMYVLPKFMGLNGVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEE
FCQTAADGLAFLETIIMAIWLKKQFKKEVSQESTLGDVKEIS
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA