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The map label for this gene is cspR [H]

Identifier: 28210123

GI number: 28210123

Start: 354695

End: 355183

Strand: Direct

Name: cspR [H]

Synonym: CTC00367

Alternate gene names: 28210123

Gene position: 354695-355183 (Clockwise)

Preceding gene: 28210122

Following gene: 28210124

Centisome position: 12.67

GC content: 29.86

Gene sequence:

>489_bases
ATGTATAAGGGGGAGAATATCTTGAATTTAAATATAGTTTTGTTCCAACCAGAAATTCCACAAAATACAGGTAATATAGC
AAGAACTTGTGTTTTAACAGATTCTAAGCTTCATTTAATAAAGCCATTAGGTTTTAGTTTAGATGAAAAGCATTTAAAGA
GGGCAGGATTGGATTACTGGTCTCAATTATCATTGGAAATACATGAATCTTATGAAGATTTAAGAAAAAAATATAAAGAT
GGTAAATTTTATTTTTCTACCACTCATAGTACTAAAACTTATAGTGATGTAGAATTTGAAGAAGGGGATTTTATAGTTTT
TGGAAGGGAAACTTCAGGGTTGCCAGATTATGTAAGAGAGGATAATAGGGATACATGTATAAAAGTACCTATGATAAAAA
GTAGTACTAGATCCTTAAATTTATCCAATACTGTAGCTATAGTAACTTATGAAGCATTAAGGCAAATGAACTTCCCCAAC
ATGAAATAG

Upstream 100 bases:

>100_bases
ACGAAGATGGTGTAGCAAAAGCTATAGAAAAATTTATAATATAAAACTTTACTATTTTTGAAGAATAGAGTAAGATATGT
TATGTAAATTTTAATTCCTT

Downstream 100 bases:

>100_bases
AGGGGGTTTCAGTTTATGAATAAAATTAAAATAATAACAGACAGTACGGCAGATTTATCAAAAGAAATAATAGATAAATA
TGATATAGAAGTTATTCCTT

Product: rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MYKGENILNLNIVLFQPEIPQNTGNIARTCVLTDSKLHLIKPLGFSLDEKHLKRAGLDYWSQLSLEIHESYEDLRKKYKD
GKFYFSTTHSTKTYSDVEFEEGDFIVFGRETSGLPDYVREDNRDTCIKVPMIKSSTRSLNLSNTVAIVTYEALRQMNFPN
MK

Sequences:

>Translated_162_residues
MYKGENILNLNIVLFQPEIPQNTGNIARTCVLTDSKLHLIKPLGFSLDEKHLKRAGLDYWSQLSLEIHESYEDLRKKYKD
GKFYFSTTHSTKTYSDVEFEEGDFIVFGRETSGLPDYVREDNRDTCIKVPMIKSSTRSLNLSNTVAIVTYEALRQMNFPN
MK
>Mature_162_residues
MYKGENILNLNIVLFQPEIPQNTGNIARTCVLTDSKLHLIKPLGFSLDEKHLKRAGLDYWSQLSLEIHESYEDLRKKYKD
GKFYFSTTHSTKTYSDVEFEEGDFIVFGRETSGLPDYVREDNRDTCIKVPMIKSSTRSLNLSNTVAIVTYEALRQMNFPN
MK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=43.421052631579, Blast_Score=138, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18754; Mature: 18754

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGENILNLNIVLFQPEIPQNTGNIARTCVLTDSKLHLIKPLGFSLDEKHLKRAGLDYW
CCCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCH
SQLSLEIHESYEDLRKKYKDGKFYFSTTHSTKTYSDVEFEEGDFIVFGRETSGLPDYVRE
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCHHHHC
DNRDTCIKVPMIKSSTRSLNLSNTVAIVTYEALRQMNFPNMK
CCCCCEEEEEEECCCCCEECCCCEEEEEEHHHHHHCCCCCCC
>Mature Secondary Structure
MYKGENILNLNIVLFQPEIPQNTGNIARTCVLTDSKLHLIKPLGFSLDEKHLKRAGLDYW
CCCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCH
SQLSLEIHESYEDLRKKYKDGKFYFSTTHSTKTYSDVEFEEGDFIVFGRETSGLPDYVRE
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCHHHHC
DNRDTCIKVPMIKSSTRSLNLSNTVAIVTYEALRQMNFPNMK
CCCCCEEEEEEECCCCCEECCCCEEEEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]