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The map label for this gene is ytsJ [H]

Identifier: 28210112

GI number: 28210112

Start: 341149

End: 342318

Strand: Direct

Name: ytsJ [H]

Synonym: CTC00356

Alternate gene names: 28210112

Gene position: 341149-342318 (Clockwise)

Preceding gene: 28210111

Following gene: 28210114

Centisome position: 12.19

GC content: 32.91

Gene sequence:

>1170_bases
ATGGATGTAAAAGAAATGGCTTTAAAATTACACTCGGAAAAGAAGGGAAAATTAGAAGTTGTAGGTAAGATATCTGTTAA
GAATAGGGAAGATTTAGCATTGGCATATACGCCAGGAGTTGCTGAACCTTGTGTGCATATATCAAAGGATAAAAATAAGG
TATATGAATATACTACAAAAGGTAATATGGTTGCAGTGGTTACTAATGGAACAGCAGTTTTAGGATTAGGAAACATTGGT
CCAGAAGCTGCATTACCTGTAATGGAGGGTAAAGCACTTCTTTTTAAAGAGTTTGCAAATGTAGATGCTTTTCCTATATG
TTTGGATACTGAAGATCCAGAAGAAATTATAAGAACTGTAAAATTAATGGCACCAGGCTTCGGTGGTATTAATTTAGAAG
ATATAAAAGCACCTGAATGTTTTTATATAGAAGAAAGACTTAAAAAAGAATTGGATATACCAGTATTCCATGATGATCAA
CATGGAACAGCTATAGTTGTATTAGCAGGTATATATAATGCTTTAAAAATAGTAGATAAAAAATTAGAAGAAGCAAAAAT
TCTTATAAATGGAGCGGGCTCAGCAGGAATCGCAATATGTAAGCTTTTATTAAATGCTGGAGCTTGTAATATTGTTATGT
GCGATATAAATGGATCCCTAGTAGAGGGAGATGAGAATTTAAATCCTGCCCAAAAAGAAATAGCAAAAGTAACTAATAAA
GAAAAAGAAAAAGGAAAGCTTGTTGATGTAATAAAAGATAAGGATATATTTATAGGAGTTTCAGGGCCTAAAATATTAAC
AAAGGAAATGGTTTCTACTATGGCAAAGGATAGTATAGTTTTTGCTATGGCTAATCCAGAACCAGAGATACTTCCAGATG
AAGCTAAAGCTGGAGGAGCTAGAGTTGTTGCAACAGGAAGATCTGATTTTCCAAATCAAATAAACAATGTTTTAGTGTTC
CCAGGTATATTTAGGGGAGCATTGGATGTAAAGGCTAGAGAAATAAACGAAGAGATGAAAATTGCAGCAGCAAGAGGAGT
GGCAAATCTTATAAAAGAAGAAGACTTAAATGAAGAATATATAATCCCAGATCCATTTAATAAAGAAGTGGCAGAATCTG
TATCAAAAGAAGTTAGAAGGATAGCAAAAGAAATGAATATCTGTAAATAA

Upstream 100 bases:

>100_bases
TTGAGGGGTAAAGCTTCAAAAGATAAACACTTGGAAGGAGGGTAGTATTAAATTTTAAAGATGAAAGAATAAAATCATTT
TTAAAATTTAATAAAATGCT

Downstream 100 bases:

>100_bases
ATATATAAGAAAAGAGTGTCTATAATTTAAAAATGTAGACACTCTTTTCTTTAAACTAAATTAAGTTTTTTCTAAGATAA
TCTAATGCTACAGTAACAGT

Product: NAD-dependent malic enzyme

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MDVKEMALKLHSEKKGKLEVVGKISVKNREDLALAYTPGVAEPCVHISKDKNKVYEYTTKGNMVAVVTNGTAVLGLGNIG
PEAALPVMEGKALLFKEFANVDAFPICLDTEDPEEIIRTVKLMAPGFGGINLEDIKAPECFYIEERLKKELDIPVFHDDQ
HGTAIVVLAGIYNALKIVDKKLEEAKILINGAGSAGIAICKLLLNAGACNIVMCDINGSLVEGDENLNPAQKEIAKVTNK
EKEKGKLVDVIKDKDIFIGVSGPKILTKEMVSTMAKDSIVFAMANPEPEILPDEAKAGGARVVATGRSDFPNQINNVLVF
PGIFRGALDVKAREINEEMKIAAARGVANLIKEEDLNEEYIIPDPFNKEVAESVSKEVRRIAKEMNICK

Sequences:

>Translated_389_residues
MDVKEMALKLHSEKKGKLEVVGKISVKNREDLALAYTPGVAEPCVHISKDKNKVYEYTTKGNMVAVVTNGTAVLGLGNIG
PEAALPVMEGKALLFKEFANVDAFPICLDTEDPEEIIRTVKLMAPGFGGINLEDIKAPECFYIEERLKKELDIPVFHDDQ
HGTAIVVLAGIYNALKIVDKKLEEAKILINGAGSAGIAICKLLLNAGACNIVMCDINGSLVEGDENLNPAQKEIAKVTNK
EKEKGKLVDVIKDKDIFIGVSGPKILTKEMVSTMAKDSIVFAMANPEPEILPDEAKAGGARVVATGRSDFPNQINNVLVF
PGIFRGALDVKAREINEEMKIAAARGVANLIKEEDLNEEYIIPDPFNKEVAESVSKEVRRIAKEMNICK
>Mature_389_residues
MDVKEMALKLHSEKKGKLEVVGKISVKNREDLALAYTPGVAEPCVHISKDKNKVYEYTTKGNMVAVVTNGTAVLGLGNIG
PEAALPVMEGKALLFKEFANVDAFPICLDTEDPEEIIRTVKLMAPGFGGINLEDIKAPECFYIEERLKKELDIPVFHDDQ
HGTAIVVLAGIYNALKIVDKKLEEAKILINGAGSAGIAICKLLLNAGACNIVMCDINGSLVEGDENLNPAQKEIAKVTNK
EKEKGKLVDVIKDKDIFIGVSGPKILTKEMVSTMAKDSIVFAMANPEPEILPDEAKAGGARVVATGRSDFPNQINNVLVF
PGIFRGALDVKAREINEEMKIAAARGVANLIKEEDLNEEYIIPDPFNKEVAESVSKEVRRIAKEMNICK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=438, Percent_Identity=24.8858447488584, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI62420880, Length=438, Percent_Identity=24.8858447488584, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI239049447, Length=438, Percent_Identity=24.8858447488584, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4505143, Length=416, Percent_Identity=24.0384615384615, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4505145, Length=412, Percent_Identity=25, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788806, Length=405, Percent_Identity=48.8888888888889, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI87081919, Length=405, Percent_Identity=26.1728395061728, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537199, Length=408, Percent_Identity=25.4901960784314, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=267, Percent_Identity=29.9625468164794, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362674, Length=415, Percent_Identity=26.7469879518072, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI281362672, Length=415, Percent_Identity=26.7469879518072, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356279, Length=415, Percent_Identity=26.7469879518072, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI78707242, Length=407, Percent_Identity=26.7813267813268, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707238, Length=407, Percent_Identity=26.7813267813268, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707240, Length=430, Percent_Identity=26.2790697674419, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707232, Length=374, Percent_Identity=27.5401069518717, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281363505, Length=374, Percent_Identity=27.5401069518717, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI78707236, Length=374, Percent_Identity=27.5401069518717, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281363503, Length=374, Percent_Identity=27.5401069518717, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42269; Mature: 42269

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00331 MALIC_ENZYMES ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKEMALKLHSEKKGKLEVVGKISVKNREDLALAYTPGVAEPCVHISKDKNKVYEYTTK
CCHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCEEECCCCCCEEEEECC
GNMVAVVTNGTAVLGLGNIGPEAALPVMEGKALLFKEFANVDAFPICLDTEDPEEIIRTV
CCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KLMAPGFGGINLEDIKAPECFYIEERLKKELDIPVFHDDQHGTAIVVLAGIYNALKIVDK
HHHCCCCCCCCHHHCCCCCEEHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEAKILINGAGSAGIAICKLLLNAGACNIVMCDINGSLVEGDENLNPAQKEIAKVTNK
HHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHCC
EKEKGKLVDVIKDKDIFIGVSGPKILTKEMVSTMAKDSIVFAMANPEPEILPDEAKAGGA
CHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCE
RVVATGRSDFPNQINNVLVFPGIFRGALDVKAREINEEMKIAAARGVANLIKEEDLNEEY
EEEEECCCCCCHHHCCEEEECCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIPDPFNKEVAESVSKEVRRIAKEMNICK
ECCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDVKEMALKLHSEKKGKLEVVGKISVKNREDLALAYTPGVAEPCVHISKDKNKVYEYTTK
CCHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCEEECCCCCCEEEEECC
GNMVAVVTNGTAVLGLGNIGPEAALPVMEGKALLFKEFANVDAFPICLDTEDPEEIIRTV
CCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KLMAPGFGGINLEDIKAPECFYIEERLKKELDIPVFHDDQHGTAIVVLAGIYNALKIVDK
HHHCCCCCCCCHHHCCCCCEEHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEAKILINGAGSAGIAICKLLLNAGACNIVMCDINGSLVEGDENLNPAQKEIAKVTNK
HHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHCC
EKEKGKLVDVIKDKDIFIGVSGPKILTKEMVSTMAKDSIVFAMANPEPEILPDEAKAGGA
CHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCE
RVVATGRSDFPNQINNVLVFPGIFRGALDVKAREINEEMKIAAARGVANLIKEEDLNEEY
EEEEECCCCCCHHHCCEEEECCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIPDPFNKEVAESVSKEVRRIAKEMNICK
ECCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]