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The map label for this gene is nth [H]

Identifier: 28210111

GI number: 28210111

Start: 340305

End: 340943

Strand: Direct

Name: nth [H]

Synonym: CTC00355

Alternate gene names: 28210111

Gene position: 340305-340943 (Clockwise)

Preceding gene: 28210110

Following gene: 28210112

Centisome position: 12.16

GC content: 31.3

Gene sequence:

>639_bases
TTGAATAAAAAAATAATAAAAAAGGTTATAGAAACACTAAGTAGAACCTATCCAGAAGCAAAATGTGAATTAGATTTTAA
GTCACCCTATGAACTTTTAGTAGCAACTATATTGTCGGCTCAATGTACAGATAAGAGAGTAAATAAAGTAACCTCTGAAC
TTTTTAAAGGATACAATACACCAGAAAAAATCATTGAATTATCACAAGAGGAATTAGGTGAAAAGATAAAAAGCTGTGGT
TTCTACAATAATAAAAGTAAAAATATATTAGGTGCTACACAAAAAATTCTTGAGAAGTTTAAAGGAAAAGTCCCAAAAAC
TATGGAGGAACTAATGTCTCTTCCAGGGGTAGGAAGGAAAACTGCTAATGTAGTACTATCTAATGCATTTGGAGTGCCTG
CAATAGCTGTGGATACACATGTGTTTAGAGTATCAAATAGAACTGGTATTGCTAAGGGTAAAAACCCTGATGAAGTAGAA
ATGGAATTAATGAAAAATATAGACAAAGATATGTGGAGTATAACCCATCATTATTTAATATGGCATGGAAGATATACTTG
CAAGTCAAGAAAACCACAATGTGAAGAGTGTCCTATAGCACCTTATTGTGAATATTTTACTGGAATAGAAAAAAGTTAA

Upstream 100 bases:

>100_bases
GAGAGAATAAGCACGATTATGAAGAGATGGTTTTAGAATATTTTATGAAAAGTATAGCTAGACTATTACCAGAAGAATAT
CAAGGAGTGTATAAAATAAA

Downstream 100 bases:

>100_bases
GAAAATATTAAAGGAATTTTTAAATTTATGAAGAATACTAAATATTGAAGGAAAATATTCAAAAAGTGTTTTCAAAAGAA
AGCCAAATACTTTAAGATGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNKKIIKKVIETLSRTYPEAKCELDFKSPYELLVATILSAQCTDKRVNKVTSELFKGYNTPEKIIELSQEELGEKIKSCG
FYNNKSKNILGATQKILEKFKGKVPKTMEELMSLPGVGRKTANVVLSNAFGVPAIAVDTHVFRVSNRTGIAKGKNPDEVE
MELMKNIDKDMWSITHHYLIWHGRYTCKSRKPQCEECPIAPYCEYFTGIEKS

Sequences:

>Translated_212_residues
MNKKIIKKVIETLSRTYPEAKCELDFKSPYELLVATILSAQCTDKRVNKVTSELFKGYNTPEKIIELSQEELGEKIKSCG
FYNNKSKNILGATQKILEKFKGKVPKTMEELMSLPGVGRKTANVVLSNAFGVPAIAVDTHVFRVSNRTGIAKGKNPDEVE
MELMKNIDKDMWSITHHYLIWHGRYTCKSRKPQCEECPIAPYCEYFTGIEKS
>Mature_212_residues
MNKKIIKKVIETLSRTYPEAKCELDFKSPYELLVATILSAQCTDKRVNKVTSELFKGYNTPEKIIELSQEELGEKIKSCG
FYNNKSKNILGATQKILEKFKGKVPKTMEELMSLPGVGRKTANVVLSNAFGVPAIAVDTHVFRVSNRTGIAKGKNPDEVE
MELMKNIDKDMWSITHHYLIWHGRYTCKSRKPQCEECPIAPYCEYFTGIEKS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=183, Percent_Identity=30.6010928961749, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=49.2682926829268, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1789331, Length=189, Percent_Identity=24.8677248677249, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=171, Percent_Identity=36.2573099415205, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=135, Percent_Identity=34.0740740740741, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=26.7379679144385, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=171, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24089; Mature: 24089

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIIKKVIETLSRTYPEAKCELDFKSPYELLVATILSAQCTDKRVNKVTSELFKGYNT
CCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEKIIELSQEELGEKIKSCGFYNNKSKNILGATQKILEKFKGKVPKTMEELMSLPGVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCH
TANVVLSNAFGVPAIAVDTHVFRVSNRTGIAKGKNPDEVEMELMKNIDKDMWSITHHYLI
HHHHHHHCCCCCCEEEHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
WHGRYTCKSRKPQCEECPIAPYCEYFTGIEKS
ECCEEECCCCCCCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MNKKIIKKVIETLSRTYPEAKCELDFKSPYELLVATILSAQCTDKRVNKVTSELFKGYNT
CCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEKIIELSQEELGEKIKSCGFYNNKSKNILGATQKILEKFKGKVPKTMEELMSLPGVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCH
TANVVLSNAFGVPAIAVDTHVFRVSNRTGIAKGKNPDEVEMELMKNIDKDMWSITHHYLI
HHHHHHHCCCCCCEEEHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
WHGRYTCKSRKPQCEECPIAPYCEYFTGIEKS
ECCEEECCCCCCCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]