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The map label for this gene is sleB [H]

Identifier: 28210107

GI number: 28210107

Start: 336875

End: 337642

Strand: Direct

Name: sleB [H]

Synonym: CTC00350

Alternate gene names: 28210107

Gene position: 336875-337642 (Clockwise)

Preceding gene: 28210106

Following gene: 28210108

Centisome position: 12.03

GC content: 34.24

Gene sequence:

>768_bases
ATGTATTTTTTTCTATTCTTCGGTAAAAATAAGATAGAAATACATATTATTAGTAGGGAGTGTATTAGTATGAAGAATAG
GAGAATTAGTTTTAAAAAGGGTATATTATATTTTTTAATAGCAGCCATATGTTATTCTGCTGTGCATGTGTTCTTTAGTC
CCTATAGTAAAGCTTTAAATGTAATAGCATATAATTATGGTTCAAAAGGTGGGGACGTATCTGAAATACAAAAAAAATTA
AAAAATTGGGGATATTATGATGGAAGTGTAGATGGAATATATGGCTATAAAACCTTTCTTGCGGTAAAGAAATTTCAGGG
TAAAAATGGATTAAATGTAGATGGAGTTGCAGGAAATAAAACATTGGCTGCATTAGGAATAAATGTAGCTCAGGCACAGG
GGAGCAATACATCTAATAACCAAGATATAAATTTATTAGCAAGACTTATAAATGGAGAAGCCAGGGGAGAACCTTATGAA
GGGCAAGTAGCTGTAGGAGCAGTTATTTTAAATAGAACAAGGGATCCACGTTTCCCCAACACTATAGCAGGGGTTGTATA
TCAGCCACTAGCCTTTACAGCAATAGCAGATGGTCAAGTAAATGCAGCTATGGAGCAAAGTTCTATGAAGGCAGCTATGG
ATGCAATTAATGGATGGGATCCATCAGGGGGGGCTGTATATTATTTTAATCCAGCTACAGCAACCAGTTCATGGATATGG
TCTAGACCTTTAATAAAAGTAATAGGAAAACATAGATTTTGCAAATAA

Upstream 100 bases:

>100_bases
ATTTTTTCGTAATGATAATGGAGAAAAAATATCCACAATTGTTCATTCTTCATTATTCCTTGTTCATTAATAAAAATTTT
GTTACGCAAAATTTTTATTA

Downstream 100 bases:

>100_bases
AAATAGGTTGACAAATAATAAATATAGAATATAATAATTAATATAGAATTCTTAGTGATTTACTCGGAAAATATAATTTG
AAAAAATACATTGAAAGAGG

Product: spore-cortex-lytic enzyme precursor

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MYFFLFFGKNKIEIHIISRECISMKNRRISFKKGILYFLIAAICYSAVHVFFSPYSKALNVIAYNYGSKGGDVSEIQKKL
KNWGYYDGSVDGIYGYKTFLAVKKFQGKNGLNVDGVAGNKTLAALGINVAQAQGSNTSNNQDINLLARLINGEARGEPYE
GQVAVGAVILNRTRDPRFPNTIAGVVYQPLAFTAIADGQVNAAMEQSSMKAAMDAINGWDPSGGAVYYFNPATATSSWIW
SRPLIKVIGKHRFCK

Sequences:

>Translated_255_residues
MYFFLFFGKNKIEIHIISRECISMKNRRISFKKGILYFLIAAICYSAVHVFFSPYSKALNVIAYNYGSKGGDVSEIQKKL
KNWGYYDGSVDGIYGYKTFLAVKKFQGKNGLNVDGVAGNKTLAALGINVAQAQGSNTSNNQDINLLARLINGEARGEPYE
GQVAVGAVILNRTRDPRFPNTIAGVVYQPLAFTAIADGQVNAAMEQSSMKAAMDAINGWDPSGGAVYYFNPATATSSWIW
SRPLIKVIGKHRFCK
>Mature_255_residues
MYFFLFFGKNKIEIHIISRECISMKNRRISFKKGILYFLIAAICYSAVHVFFSPYSKALNVIAYNYGSKGGDVSEIQKKL
KNWGYYDGSVDGIYGYKTFLAVKKFQGKNGLNVDGVAGNKTLAALGINVAQAQGSNTSNNQDINLLARLINGEARGEPYE
GQVAVGAVILNRTRDPRFPNTIAGVVYQPLAFTAIADGQVNAAMEQSSMKAAMDAINGWDPSGGAVYYFNPATATSSWIW
SRPLIKVIGKHRFCK

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 28026; Mature: 28026

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFFLFFGKNKIEIHIISRECISMKNRRISFKKGILYFLIAAICYSAVHVFFSPYSKALN
CEEEEEECCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VIAYNYGSKGGDVSEIQKKLKNWGYYDGSVDGIYGYKTFLAVKKFQGKNGLNVDGVAGNK
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
TLAALGINVAQAQGSNTSNNQDINLLARLINGEARGEPYEGQVAVGAVILNRTRDPRFPN
EEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
TIAGVVYQPLAFTAIADGQVNAAMEQSSMKAAMDAINGWDPSGGAVYYFNPATATSSWIW
HHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHH
SRPLIKVIGKHRFCK
HCHHHHHHCCCCCCC
>Mature Secondary Structure
MYFFLFFGKNKIEIHIISRECISMKNRRISFKKGILYFLIAAICYSAVHVFFSPYSKALN
CEEEEEECCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VIAYNYGSKGGDVSEIQKKLKNWGYYDGSVDGIYGYKTFLAVKKFQGKNGLNVDGVAGNK
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
TLAALGINVAQAQGSNTSNNQDINLLARLINGEARGEPYEGQVAVGAVILNRTRDPRFPN
EEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
TIAGVVYQPLAFTAIADGQVNAAMEQSSMKAAMDAINGWDPSGGAVYYFNPATATSSWIW
HHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHH
SRPLIKVIGKHRFCK
HCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]