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The map label for this gene is thlA [H]

Identifier: 28210073

GI number: 28210073

Start: 294440

End: 295615

Strand: Direct

Name: thlA [H]

Synonym: CTC00312

Alternate gene names: 28210073

Gene position: 294440-295615 (Clockwise)

Preceding gene: 28210072

Following gene: 28210074

Centisome position: 10.52

GC content: 36.82

Gene sequence:

>1176_bases
ATGAAAGAAGTTGTTATTGTTAGTGCAGTTAGAACTGCCATGGGTAAATTTGGAGGAACTTTAAAAGATGTACCTGCAGT
TGAATTAGGTGCTACAGTTATTAAAGAAGCAATAAACAGAGCGGGAATTAAACCTGAAATTATAGACGAAGTTATAATGG
GAAATGTTATCCAAGCAGGACTTGGACAAAGTCCAGGAAGACAAGCAGCTGTAAAAGCTGGAATACCTGTTGAAGTACCA
GCATTTACTTTAAATAAGGTTTGCGGATCAGGATTAAGAGCAGTTGGGCTAGCAGCTCAAATGATAAAAGCTGGAGATGC
TGATGTAGTAATAGCTGGTGGAATGGAAAACATGTCTGCAGCACCATATGTTTTACCAAACGCTAGATGGGGACAAAGAA
TGTTTGATGGTAAAATGGTAGATACTATGGTAAAGGATGGTTTATGGGAATCATTCAATGATTATCATATGGGAATGACA
GCAGAAAACATAGCAGAGAAATGGGAATTAACAAGAGAAATGCAAGATGAGTTTGCATGTGCTTCACAAAATAAAGCTGA
AAAAGCTATTAAAGAAGGAAAGTTTAAAGACGAAATCGTTCCAGTAGTTATAAAAACTAGAAAAGGTGAAGTTGTATTTG
ATACAGATGAGTTCCCAAGATTTGGTGCTACTGTAGAATCATTAGCAAAGTTAAAACCAGCATTTAAAAAAGATGGAACA
GTTACAGCAGGAAATGCATCAGGTATAAATGATGGTGCAGCTGCGTTAGTTATAATGAGTGCAGAAAAAGCTGAAGAACT
AGGATTAAAACCATTAGCTAAGATAGCTTCCTATGGTTCAAAAGGATTAGACCCAGCTTATATGGGATATGGACCAGTAG
GTGCAACAAAAGTTGCTCTTGAAAAAGCTGGATGGAAAGTAGAAGACTTAGATTTAATCGAAGCTAATGAAGCTTTTGCG
TCACAAAGTTTAGCTGTTGCTAAAGATCTAGGATTTGATATGGAAAAAGTAAATGTTAATGGTGGAGCTATAGCACTTGG
ACATCCAGTGGGATGCTCTGGAGCAAGAATATTAGTTACATTACTACATGAAATGCAAAGAAGAGATTCTAAAAAAGGTT
TAGCTACTCTTTGCATAGGTGGAGGAATGGGAACAGCTCTTCTTGTAGAAAGATAA

Upstream 100 bases:

>100_bases
AATATGGGGTTATAATATAAATAGTTTAAAAGAAATTTGTTAATTTTTTAACTAAAGGTGATATAAAGCCAAAATTTTAA
ATACTTTGGGAGGGTTTTAA

Downstream 100 bases:

>100_bases
TAAAATCAAATATAAATTAAGCCGTTAGGAATATTCCTAACGGCTTTTGTGTTACAAAATAAAAGTTGTAAAAAAACTCT
CATTTTGTAACACTTTTTTT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKEVVIVSAVRTAMGKFGGTLKDVPAVELGATVIKEAINRAGIKPEIIDEVIMGNVIQAGLGQSPGRQAAVKAGIPVEVP
AFTLNKVCGSGLRAVGLAAQMIKAGDADVVIAGGMENMSAAPYVLPNARWGQRMFDGKMVDTMVKDGLWESFNDYHMGMT
AENIAEKWELTREMQDEFACASQNKAEKAIKEGKFKDEIVPVVIKTRKGEVVFDTDEFPRFGATVESLAKLKPAFKKDGT
VTAGNASGINDGAAALVIMSAEKAEELGLKPLAKIASYGSKGLDPAYMGYGPVGATKVALEKAGWKVEDLDLIEANEAFA
SQSLAVAKDLGFDMEKVNVNGGAIALGHPVGCSGARILVTLLHEMQRRDSKKGLATLCIGGGMGTALLVER

Sequences:

>Translated_391_residues
MKEVVIVSAVRTAMGKFGGTLKDVPAVELGATVIKEAINRAGIKPEIIDEVIMGNVIQAGLGQSPGRQAAVKAGIPVEVP
AFTLNKVCGSGLRAVGLAAQMIKAGDADVVIAGGMENMSAAPYVLPNARWGQRMFDGKMVDTMVKDGLWESFNDYHMGMT
AENIAEKWELTREMQDEFACASQNKAEKAIKEGKFKDEIVPVVIKTRKGEVVFDTDEFPRFGATVESLAKLKPAFKKDGT
VTAGNASGINDGAAALVIMSAEKAEELGLKPLAKIASYGSKGLDPAYMGYGPVGATKVALEKAGWKVEDLDLIEANEAFA
SQSLAVAKDLGFDMEKVNVNGGAIALGHPVGCSGARILVTLLHEMQRRDSKKGLATLCIGGGMGTALLVER
>Mature_391_residues
MKEVVIVSAVRTAMGKFGGTLKDVPAVELGATVIKEAINRAGIKPEIIDEVIMGNVIQAGLGQSPGRQAAVKAGIPVEVP
AFTLNKVCGSGLRAVGLAAQMIKAGDADVVIAGGMENMSAAPYVLPNARWGQRMFDGKMVDTMVKDGLWESFNDYHMGMT
AENIAEKWELTREMQDEFACASQNKAEKAIKEGKFKDEIVPVVIKTRKGEVVFDTDEFPRFGATVESLAKLKPAFKKDGT
VTAGNASGINDGAAALVIMSAEKAEELGLKPLAKIASYGSKGLDPAYMGYGPVGATKVALEKAGWKVEDLDLIEANEAFA
SQSLAVAKDLGFDMEKVNVNGGAIALGHPVGCSGARILVTLLHEMQRRDSKKGLATLCIGGGMGTALLVER

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=54.8717948717949, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=45.3164556962025, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI4557237, Length=396, Percent_Identity=44.9494949494949, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=43.2911392405063, Blast_Score=285, Evalue=3e-77,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=32.6291079812207, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=32.8205128205128, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=63.0102040816327, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=56.2659846547315, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=45.6359102244389, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=43.859649122807, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=33.9853300733496, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI133906874, Length=395, Percent_Identity=48.1012658227848, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25147385, Length=392, Percent_Identity=41.3265306122449, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=42.1319796954315, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=32.5699745547074, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=33.4117647058824, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=45.5, Blast_Score=316, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=40.3508771929825, Blast_Score=254, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=54.2199488491049, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=45.7800511508951, Blast_Score=333, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24640423, Length=400, Percent_Identity=45.75, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=32.5581395348837, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41229; Mature: 41229

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVVIVSAVRTAMGKFGGTLKDVPAVELGATVIKEAINRAGIKPEIIDEVIMGNVIQAG
CCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
LGQSPGRQAAVKAGIPVEVPAFTLNKVCGSGLRAVGLAAQMIKAGDADVVIAGGMENMSA
CCCCCCCCHHEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APYVLPNARWGQRMFDGKMVDTMVKDGLWESFNDYHMGMTAENIAEKWELTREMQDEFAC
CCEECCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ASQNKAEKAIKEGKFKDEIVPVVIKTRKGEVVFDTDEFPRFGATVESLAKLKPAFKKDGT
HCCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHCHHHHCCCC
VTAGNASGINDGAAALVIMSAEKAEELGLKPLAKIASYGSKGLDPAYMGYGPVGATKVAL
EEECCCCCCCCCCEEEEEEECHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHH
EKAGWKVEDLDLIEANEAFASQSLAVAKDLGFDMEKVNVNGGAIALGHPVGCSGARILVT
HHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHH
LLHEMQRRDSKKGLATLCIGGGMGTALLVER
HHHHHHHHHHHCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MKEVVIVSAVRTAMGKFGGTLKDVPAVELGATVIKEAINRAGIKPEIIDEVIMGNVIQAG
CCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
LGQSPGRQAAVKAGIPVEVPAFTLNKVCGSGLRAVGLAAQMIKAGDADVVIAGGMENMSA
CCCCCCCCHHEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APYVLPNARWGQRMFDGKMVDTMVKDGLWESFNDYHMGMTAENIAEKWELTREMQDEFAC
CCEECCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ASQNKAEKAIKEGKFKDEIVPVVIKTRKGEVVFDTDEFPRFGATVESLAKLKPAFKKDGT
HCCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHCHHHHCCCC
VTAGNASGINDGAAALVIMSAEKAEELGLKPLAKIASYGSKGLDPAYMGYGPVGATKVAL
EEECCCCCCCCCCEEEEEEECHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHH
EKAGWKVEDLDLIEANEAFASQSLAVAKDLGFDMEKVNVNGGAIALGHPVGCSGARILVT
HHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHH
LLHEMQRRDSKKGLATLCIGGGMGTALLVER
HHHHHHHHHHHCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]