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The map label for this gene is traC [H]

Identifier: 28198695

GI number: 28198695

Start: 977346

End: 978446

Strand: Reverse

Name: traC [H]

Synonym: PD0790

Alternate gene names: 28198695

Gene position: 978446-977346 (Counterclockwise)

Preceding gene: 28198696

Following gene: 28198689

Centisome position: 38.83

GC content: 51.41

Gene sequence:

>1101_bases
TTGAAGCCGTGGATTGCTACGGAGATCATGAGTCGGCAAGTTCCGGCACTTTCTCCAGAAAAAGAATTTGCAGAGACGTT
ACGAAGCATGGGATGTCTCGTGAGTGGTGAACATCCCTTCATGGACGCTAAGAAATACCGTATTGAGGTCGAAGGAGACA
AAAAGGGGGGAAAGGCTGGTTTTTATGTCGCTTACCTCGACGGTCACCCATACGGCTATATCAAGAACAACCGTACTGGC
ATCGAATTGAAGTGGAAAAGCAAAGGGTACTCGCTTGATCCAGAGCAAAAGGCCGCAATGCAAGCGGAGGCAACCGCAAA
ATTGGCTGCACGCGCAGAGCAGCAGCAGCAAGAACAAGAGGCTGTCGCGCAACATGTCGTAAAGCAGATGGATGATCTTG
TGACTGTTATCGAGCCGACGCCCTATCTAAAAGCTAAAGGCATTACGCCACAGGCTGGTATATACACTGACAGTGATGGC
CAGAAGACCTACATTCCAGCTATCGATTCGGATGGTAAGCAATGGACGATGCAATACATCCAAGAGGACGGTACAAAGCG
ATTTGCAAAAGGGGGGCGTAAGGAGGGTTGTTTTCACCCCATAGGGGGTCTTGCTGCTATCGCAGCAGCGCCCGTGATCG
TCATTGGCCAGGGCTATGCCACTGTGGCGCAAGTGTCTCAGGCATTGGGTTATGGGACCGTTGCCGCATTTGATGCTGGC
AATCTGGAAGGTGTTGCCAAAGCATTGCACAAGTCTTTCCCAGACAAGCCTATTCTGATTGCCGGTGACGATGATCAGTC
TTTACCTAACAATCCAGGAAGGACCAAGGCTCAAGAGGCAGCGCGTGCTGTAGACGGTACGGCGTTCTTCCCAATCTTCG
CGCCAGGTGAACAGGCGGATAATCCGAAAGGCTTCACCGATTTCAATGATCTTGCCACTAAGAGTGCGCTCGGTATCGAA
GCGGTTGAACGGCAGGTACATGCGGCTGTTAGCAAGGCTATCGATAATCATCAGAAACGTGTTCAGAAGCAGACTCAACA
ACAAGGGCAACAAGGGCAACACGAGCAGAAGCAGGAACGTCGACGGCTTCGCGTTCGCTAG

Upstream 100 bases:

>100_bases
CAAATAAATCGGTGGAAAGCTTCCGAACGCTAATAATGCAGTTGATTGGGATAATGCTGCCCAATGTTGGTATGCAAATC
CTGGAGCTGATCTTGATAAG

Downstream 100 bases:

>100_bases
CAGCCAGCCAAGAGGTGGGGGAGCAGCCGACCGGCGAAGAAGTGCGCGAGGTGTATGTATCCGATCAAAATGCAAAAGTG
CATCATTGGATAGATGTATG

Product: DNA primase

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKPWIATEIMSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGHPYGYIKNNRTG
IELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDG
QKTYIPAIDSDGKQWTMQYIQEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAG
NLEGVAKALHKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFNDLATKSALGIE
AVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR

Sequences:

>Translated_366_residues
MKPWIATEIMSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGHPYGYIKNNRTG
IELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDG
QKTYIPAIDSDGKQWTMQYIQEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAG
NLEGVAKALHKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFNDLATKSALGIE
AVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR
>Mature_366_residues
MKPWIATEIMSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGHPYGYIKNNRTG
IELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDG
QKTYIPAIDSDGKQWTMQYIQEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAG
NLEGVAKALHKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFNDLATKSALGIE
AVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4643

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 39983; Mature: 39983

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPWIATEIMSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAG
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEEEEEECCCCCCCCE
FYVAYLDGHPYGYIKNNRTGIELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQE
EEEEEECCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAIDSDGKQWTMQYI
HHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHH
QEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAG
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCEEEECCC
NLEGVAKALHKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQAD
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCC
NPKGFTDFNDLATKSALGIEAVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RRLRVR
HCCCCC
>Mature Secondary Structure
MKPWIATEIMSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAG
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEEEEEECCCCCCCCE
FYVAYLDGHPYGYIKNNRTGIELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQE
EEEEEECCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAIDSDGKQWTMQYI
HHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHH
QEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAG
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCEEEECCC
NLEGVAKALHKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQAD
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCC
NPKGFTDFNDLATKSALGIEAVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RRLRVR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]