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The map label for this gene is acrA [H]

Identifier: 28198689

GI number: 28198689

Start: 966763

End: 967989

Strand: Reverse

Name: acrA [H]

Synonym: PD0784

Alternate gene names: 28198689

Gene position: 967989-966763 (Counterclockwise)

Preceding gene: 28198695

Following gene: 28198688

Centisome position: 38.42

GC content: 53.55

Gene sequence:

>1227_bases
GTGTCAGATCAACTCACAACCTCGGAGCCATTCCCGATGACTGTCCCGTTACGCGTACTCGGCTTGGTCTACACCGTCTT
ACTAGCACTTACCGCATGCAGTAAGTCCGAACAGCCGCAGATGCCAACACCAGAAGTTAGTATCGTCGAAGCAAAGCCGC
AAACAACTCCCATACAACTTGATTTAGTCGGACGTCTATCGGCATACCGCTCCGCAGACGTACGCGCACGGGTGGCTGGA
ATATTACAAAAGCGGATATACACCGAAGGCACCGAAGTAAAAGAAGGTCAGCCGCTGTTCCAAATCGACCCTGCGCCATT
TCAAGCCACGCTATTAGAAGCGCAAGGCCGTTTAGCTGCAGCAGAAGCCACGTATAAAAATGCCAAAATCGTCGCTGACC
GCGCCCGCCGCTTGTCCCCTGAGCAATACATTTCGCGCTCGGATGTAGACAATGCCGAGGCAGCAGAACGCACGGCAGCC
GCCAGTGTCGAACAAGCACGCGCCGCAATGCAGAGCGCACGCATCAACCTCAATTACGCCAACGTCACTGCCCCAATCAG
CGGTCTTGCTGGCCAACAGCGAGTCACTGAAGGAGCTTTAGTTGGATCTGGGAGCAGCACCCTACTCACAACCGTTGATC
AAATTGATCCGTTGTACGTGAACTTCTCAATGAGCAATGATGAGTTGATGCAACTACGCCAAGCACAGCACCAGGGAAGT
GTGCAATTGAGTAGCGACAATACATCGACCATCGACGTACTCCTCAGTGATGGCAGCAAATATCCACACCAAGGGACATT
GGATTTCTCCGGAGCAACTGTTGATCCGAGCACCGGCACAGTCTCACTGCGTGCAGTGCTTCCAAATACTGATCATCTCC
TGATGCCAGGAGCCTTTGTCACATTTAAAGCCAACCTCGGCCAGCGCAACAATACGATCCTGCTACCCGGCGCAGGGGTG
CAACGTGATGCGAACAGCGCTTACGTCCTGGTGATAGACAAAGATGGCAAGGTCATCCGCAAGAACGTGACCACGACAGG
GATGAAAGATAACCAGTGGTTGATTACTGCTGGACTCTCCAGCGGAGACCGTGTGATCGTTGATGGTCTCCAGAAAGTCA
AAGAAGGCGCTCCGGCCAAGGGCATCCCCTGGAAACCAGATCCCATTTCCAGCAGCGCACACGCAGCAGCCAAGACACCC
ACTCACACCGCTAAGACCGAACAATAA

Upstream 100 bases:

>100_bases
AAGCGCTCCACTTCGGGATCGCCACAATACAAACGGAGAATTTTTCAGAAGCATGCAATACATAAATAAAACGTGTCTGC
AACAACCAGGGCATATTTCT

Downstream 100 bases:

>100_bases
CGGACGCATCCACCATGCCAAAATTTTTCATTGAGCACCCAATCTTTGCCTGGGTGGTGGCGATCCTGATCTCACTTGCG
GGTGTGATCTCGATCTTCAA

Product: precursor of drug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSDQLTTSEPFPMTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSADVRARVAG
ILQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAA
ASVEQARAAMQSARINLNYANVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGS
VQLSSDNTSTIDVLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNTILLPGAGV
QRDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTP
THTAKTEQ

Sequences:

>Translated_408_residues
MSDQLTTSEPFPMTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSADVRARVAG
ILQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAA
ASVEQARAAMQSARINLNYANVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGS
VQLSSDNTSTIDVLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNTILLPGAGV
QRDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTP
THTAKTEQ
>Mature_407_residues
SDQLTTSEPFPMTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSADVRARVAGI
LQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAAA
SVEQARAAMQSARINLNYANVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGSV
QLSSDNTSTIDVLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNTILLPGAGVQ
RDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTPT
HTAKTEQ

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=42.3913043478261, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1789929, Length=373, Percent_Identity=39.142091152815, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1789665, Length=360, Percent_Identity=41.6666666666667, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI87082036, Length=339, Percent_Identity=31.2684365781711, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI87081796, Length=323, Percent_Identity=24.1486068111455, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43575; Mature: 43444

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQLTTSEPFPMTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQL
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEE
DLVGRLSAYRSADVRARVAGILQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAA
EEHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEE
AEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQSARINLNYA
EHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
NVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGS
EEECCCCCCCCCHHHCCCEEECCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCC
VQLSSDNTSTIDVLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFV
EEECCCCCCEEEEEEECCCCCCCCCEEECCCCEECCCCCEEEEEEECCCCCCEEECCEEE
TFKANLGQRNNTILLPGAGVQRDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLS
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCEECCCCCCCEEEEECCC
SGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ
CCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQLTTSEPFPMTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQL
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEE
DLVGRLSAYRSADVRARVAGILQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAA
EEHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEE
AEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQSARINLNYA
EHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
NVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGS
EEECCCCCCCCCHHHCCCEEECCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCC
VQLSSDNTSTIDVLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFV
EEECCCCCCEEEEEEECCCCCCCCCEEECCCCEECCCCCEEEEEEECCCCCCEEECCEEE
TFKANLGQRNNTILLPGAGVQRDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLS
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCEECCCCCCCEEEEECCC
SGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ
CCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]