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The map label for this gene is gidA [H]

Identifier: 28198678

GI number: 28198678

Start: 954063

End: 955952

Strand: Reverse

Name: gidA [H]

Synonym: PD0773

Alternate gene names: 28198678

Gene position: 955952-954063 (Counterclockwise)

Preceding gene: 28198679

Following gene: 28198671

Centisome position: 37.94

GC content: 57.72

Gene sequence:

>1890_bases
ATGACTGATTCCTTCTATCGCTACGACGTTATCGTCATCGGTGCTGGCCATGCCGGTAGCGAAGCCGCTCTGGCTGCCGC
ACGGACCGGAGCACACACCCTATTGCTGACCCACAATATTGAAACCATCGGTGCAATGAGCTGTAACCCAGCCATTGGTG
GCATCGGCAAGGGCCACCTGGTTAAAGAAATCGACGCACTCGGCGGAGCAATGGCACACGCCGCAGACGCCGCTGGCATC
CAATGGCGCACTCTCAACGCATCCAAAGGTCCGGCCGTACGCGCGACGCGCTGCCAAGCAGACCGCTCCTTATACCGTGC
CGCAATCCGGCAGATGATTGAAGGCCAGGCACGCCTCAACATATTCCAAGCAGAAGTGGACGACCTGCTGTTTGAAGGGG
ACACTGTATGCGGTGCGATTACACACACCGGGTTGCATTTCAAAGCGCCTGCCGTCGTCCTTACCGCCGGCACATTCCTT
GCCGGAAAAATCCATATCGGACCAACCCAATACGCCGCGGGGCGCATGGGCGACCCGCCAGCAACCATGCTAGCCGCCCG
CCTGCGGGAACGTCTATTGACAGTTGCACGGCTCAAAACAGGGACCCCACCACGGATTGATGGCCGGACGCTGAACTACA
CAGCGATGCAAGAACAACCAGGTGACGCCCCACGCCCTACCATGTCCTTCATAGGAAACTCAGCTTCCCATCCCCCTCAA
GTCAGCTGCTGGATTACTCAAACCACCGAACGCACGCATGAGATCATCCGGGCCGCCCTACACCGCTCGCCGCTATACAG
CGGGCAAATTGAGGGCACCGGCCCACGCTATTGCCCATCAATTGAAGACAAGGTGGTGCGCTTCGCCGAGAAAAATAGCC
ACCAAATTTTCGTTGAACCTGAAGGGTTGAACGTCATCGACATCTACCCGAACGGCATCTCAACCTCATTGCCCTTCGAC
GTACAGTTAGAGCTGGTACGCAGCATCCGTGGGTTCGAACAAGCACACATCACCCGCCCCGGCTATGCCATCGAATACGA
CTTCTTCGACCCACGCGGACTCAAAGCATCACTGGAAACCAAAGCAATCGCTGGACTGTTTTTCGCTGGACAGATCAACG
GCACCACCGGCTACGAAGAAGCAGCCGCCCAAGGCCTGCTGGCTGGACTCAATGCCGCACGCCATGTGCGTGGGCTATCC
TCCTGGACACCACGCCGCGACCAAGCCTACCTGGGTGTTCTTGTTGACGATTTGATCACCCATGGCACCAACGAACCGTA
CCGCATGTTCACCAGCCGAGCAGAGTACCGATTACAACTGCGCGAAGACAACGCCGACGCACGCCTCACCGCAATCGGCC
GTGACCTAGGCCTCGTCGACGATGCACGATGGGCACACTTCAACGCGAAACAGGAAGCAGTCGCCCGTGAATGTGGACGC
CTGTCCGCGCTGTGGGCCACACCAGGCAACGCCCTAGGTCGTGAAGTCAAAGAAACCCTGGGAGTGACGCTCAGCCGTGA
AACCAACATCATTGACCTTATGAAGCGGCCGGAACTGGATTACGCTGCACTGATGCGCGTCCCCTCCCTGGGTCCTGGCG
TCGATGATGCCCAAGTCGCTGAGCAAGTCGAGATCAGCGTTAAATACGCTGGTTATTTAAACCGCCAGTCCGAAGAAATT
ACCCGCCAGCAGCGCCACGAAGCAACTGCAATCCCACTTGAATTCGACTATGCCGCAGTGCGAGGACTCTCCACAGAAGT
CTTACAAAAGCTGCAACACATACAGCCACAGACAGTGGGTCAAGCGCAACGCATCCCGGGGATGACGCCTGCAGCAATCT
CGTTATTACTGGTACACCTGGAACGCATGCGTCGCAACCGCGTCGCCTGA

Upstream 100 bases:

>100_bases
TCACACAAAACACATAAAATTCCATCCAAGCTGCCTCCCTACTCCAAGCAGCCACTTCGAGTCATCCCGCCCATCCTTCT
CCACTACCGCCATTGCACAC

Downstream 100 bases:

>100_bases
TTCCAATCGCATCACTACACCTACCATGGCAAATAGCTATGCGTCACCGAGGTTGACGAAGCATTGCACCCACCAGGCTG
TGGCTGGAGAGACTCCCTAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTDSFYRYDVIVIGAGHAGSEAALAAARTGAHTLLLTHNIETIGAMSCNPAIGGIGKGHLVKEIDALGGAMAHAADAAGI
QWRTLNASKGPAVRATRCQADRSLYRAAIRQMIEGQARLNIFQAEVDDLLFEGDTVCGAITHTGLHFKAPAVVLTAGTFL
AGKIHIGPTQYAAGRMGDPPATMLAARLRERLLTVARLKTGTPPRIDGRTLNYTAMQEQPGDAPRPTMSFIGNSASHPPQ
VSCWITQTTERTHEIIRAALHRSPLYSGQIEGTGPRYCPSIEDKVVRFAEKNSHQIFVEPEGLNVIDIYPNGISTSLPFD
VQLELVRSIRGFEQAHITRPGYAIEYDFFDPRGLKASLETKAIAGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVRGLS
SWTPRRDQAYLGVLVDDLITHGTNEPYRMFTSRAEYRLQLREDNADARLTAIGRDLGLVDDARWAHFNAKQEAVARECGR
LSALWATPGNALGREVKETLGVTLSRETNIIDLMKRPELDYAALMRVPSLGPGVDDAQVAEQVEISVKYAGYLNRQSEEI
TRQQRHEATAIPLEFDYAAVRGLSTEVLQKLQHIQPQTVGQAQRIPGMTPAAISLLLVHLERMRRNRVA

Sequences:

>Translated_629_residues
MTDSFYRYDVIVIGAGHAGSEAALAAARTGAHTLLLTHNIETIGAMSCNPAIGGIGKGHLVKEIDALGGAMAHAADAAGI
QWRTLNASKGPAVRATRCQADRSLYRAAIRQMIEGQARLNIFQAEVDDLLFEGDTVCGAITHTGLHFKAPAVVLTAGTFL
AGKIHIGPTQYAAGRMGDPPATMLAARLRERLLTVARLKTGTPPRIDGRTLNYTAMQEQPGDAPRPTMSFIGNSASHPPQ
VSCWITQTTERTHEIIRAALHRSPLYSGQIEGTGPRYCPSIEDKVVRFAEKNSHQIFVEPEGLNVIDIYPNGISTSLPFD
VQLELVRSIRGFEQAHITRPGYAIEYDFFDPRGLKASLETKAIAGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVRGLS
SWTPRRDQAYLGVLVDDLITHGTNEPYRMFTSRAEYRLQLREDNADARLTAIGRDLGLVDDARWAHFNAKQEAVARECGR
LSALWATPGNALGREVKETLGVTLSRETNIIDLMKRPELDYAALMRVPSLGPGVDDAQVAEQVEISVKYAGYLNRQSEEI
TRQQRHEATAIPLEFDYAAVRGLSTEVLQKLQHIQPQTVGQAQRIPGMTPAAISLLLVHLERMRRNRVA
>Mature_628_residues
TDSFYRYDVIVIGAGHAGSEAALAAARTGAHTLLLTHNIETIGAMSCNPAIGGIGKGHLVKEIDALGGAMAHAADAAGIQ
WRTLNASKGPAVRATRCQADRSLYRAAIRQMIEGQARLNIFQAEVDDLLFEGDTVCGAITHTGLHFKAPAVVLTAGTFLA
GKIHIGPTQYAAGRMGDPPATMLAARLRERLLTVARLKTGTPPRIDGRTLNYTAMQEQPGDAPRPTMSFIGNSASHPPQV
SCWITQTTERTHEIIRAALHRSPLYSGQIEGTGPRYCPSIEDKVVRFAEKNSHQIFVEPEGLNVIDIYPNGISTSLPFDV
QLELVRSIRGFEQAHITRPGYAIEYDFFDPRGLKASLETKAIAGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVRGLSS
WTPRRDQAYLGVLVDDLITHGTNEPYRMFTSRAEYRLQLREDNADARLTAIGRDLGLVDDARWAHFNAKQEAVARECGRL
SALWATPGNALGREVKETLGVTLSRETNIIDLMKRPELDYAALMRVPSLGPGVDDAQVAEQVEISVKYAGYLNRQSEEIT
RQQRHEATAIPLEFDYAAVRGLSTEVLQKLQHIQPQTVGQAQRIPGMTPAAISLLLVHLERMRRNRVA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=646, Percent_Identity=43.1888544891641, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI19882217, Length=671, Percent_Identity=41.5797317436662, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI183227703, Length=686, Percent_Identity=40.6705539358601, Blast_Score=454, Evalue=1e-127,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=64.7154471544715, Blast_Score=810, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=39.8387096774194, Blast_Score=443, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321202, Length=638, Percent_Identity=42.0062695924765, Blast_Score=479, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24658174, Length=633, Percent_Identity=44.5497630331754, Blast_Score=480, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 68614; Mature: 68483

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSFYRYDVIVIGAGHAGSEAALAAARTGAHTLLLTHNIETIGAMSCNPAIGGIGKGHL
CCCCEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCEECCCCCCCCCCCCCCHH
VKEIDALGGAMAHAADAAGIQWRTLNASKGPAVRATRCQADRSLYRAAIRQMIEGQARLN
HHHHHHHCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEE
IFQAEVDDLLFEGDTVCGAITHTGLHFKAPAVVLTAGTFLAGKIHIGPTQYAAGRMGDPP
EHHHHHHHHHCCCCCEEEEHEECCEEEECCEEEEEECCEEEEEEEECCCHHCCCCCCCCH
ATMLAARLRERLLTVARLKTGTPPRIDGRTLNYTAMQEQPGDAPRPTMSFIGNSASHPPQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCE
VSCWITQTTERTHEIIRAALHRSPLYSGQIEGTGPRYCPSIEDKVVRFAEKNSHQIFVEP
EEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEC
EGLNVIDIYPNGISTSLPFDVQLELVRSIRGFEQAHITRPGYAIEYDFFDPRGLKASLET
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCEEEEECCCCCCCCCCCHHH
KAIAGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVRGLSSWTPRRDQAYLGVLVDDLIT
HHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
HGTNEPYRMFTSRAEYRLQLREDNADARLTAIGRDLGLVDDARWAHFNAKQEAVARECGR
CCCCCCHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
LSALWATPGNALGREVKETLGVTLSRETNIIDLMKRPELDYAALMRVPSLGPGVDDAQVA
EEEEECCCCHHHHHHHHHHHCEEEECCCCHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHH
EQVEISVKYAGYLNRQSEEITRQQRHEATAIPLEFDYAAVRGLSTEVLQKLQHIQPQTVG
HHHEEEEEECCHHCCCHHHHHHHHHHCCEECEEEECHHHHHCCCHHHHHHHHHCCCCCCC
QAQRIPGMTPAAISLLLVHLERMRRNRVA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDSFYRYDVIVIGAGHAGSEAALAAARTGAHTLLLTHNIETIGAMSCNPAIGGIGKGHL
CCCEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCEECCCCCCCCCCCCCCHH
VKEIDALGGAMAHAADAAGIQWRTLNASKGPAVRATRCQADRSLYRAAIRQMIEGQARLN
HHHHHHHCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEE
IFQAEVDDLLFEGDTVCGAITHTGLHFKAPAVVLTAGTFLAGKIHIGPTQYAAGRMGDPP
EHHHHHHHHHCCCCCEEEEHEECCEEEECCEEEEEECCEEEEEEEECCCHHCCCCCCCCH
ATMLAARLRERLLTVARLKTGTPPRIDGRTLNYTAMQEQPGDAPRPTMSFIGNSASHPPQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCE
VSCWITQTTERTHEIIRAALHRSPLYSGQIEGTGPRYCPSIEDKVVRFAEKNSHQIFVEP
EEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEC
EGLNVIDIYPNGISTSLPFDVQLELVRSIRGFEQAHITRPGYAIEYDFFDPRGLKASLET
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCEEEEECCCCCCCCCCCHHH
KAIAGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVRGLSSWTPRRDQAYLGVLVDDLIT
HHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
HGTNEPYRMFTSRAEYRLQLREDNADARLTAIGRDLGLVDDARWAHFNAKQEAVARECGR
CCCCCCHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
LSALWATPGNALGREVKETLGVTLSRETNIIDLMKRPELDYAALMRVPSLGPGVDDAQVA
EEEEECCCCHHHHHHHHHHHCEEEECCCCHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHH
EQVEISVKYAGYLNRQSEEITRQQRHEATAIPLEFDYAAVRGLSTEVLQKLQHIQPQTVG
HHHEEEEEECCHHCCCHHHHHHHHHHCCEECEEEECHHHHHCCCHHHHHHHHHCCCCCCC
QAQRIPGMTPAAISLLLVHLERMRRNRVA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]