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The map label for this gene is menD

Identifier: 27366433

GI number: 27366433

Start: 3245573

End: 3247267

Strand: Reverse

Name: menD

Synonym: VV1_3172

Alternate gene names: 27366433

Gene position: 3247267-3245573 (Counterclockwise)

Preceding gene: 27366434

Following gene: 27366432

Centisome position: 98.95

GC content: 50.86

Gene sequence:

>1695_bases
ATGAACCACGATCAAGCCGTATTGAATCGTATTTGGTGTGAAACGCTGTTTGAAGAATTGTACCGCTTTGGCGTACGAGA
TGTTTGTGTCGCACCTGGCTCACGTTCAACCCCGCTCGCCCTCGAAGCGAATGCCCATACCCGTTTGAAGTTGCATACGC
ATTTTGATGAGCGTGGATTGGGTTTTCTTGCACTTGGTTTAGCCAAAGCCAGCCAGCGGCCTGTGGCGGTGGTGGTGACA
TCGGGCACTGCGGTGGCCAATCTTTTGCCTGCAGTGGCAGAGGCAGGCTTAACCGGGGAGAAACTGGTATTACTGACCGC
AGATCGACCGATCGAGCTGGTGGGATGCGGGGCCAATCAGGCGATCGCACAGCAAGGCATCTTCTCAAATCATGTTTGTG
CTTCATTAAACTTGCCCAGTCCAAACACGCAGACGTCATTAAACTGGTTACTGACATCTGTGGATCAAGTGCTTCATCAA
CAGGCGGTCAGTGGCCATGCGGTGCACATCAACTGTCCCTTTCCTGAGCCGCTTTATTCAAATGCACCAAAGTCGATTTA
CCAGAGTTATATCGATACGGTGGATGTGTGGCGTGCAGAGGGGGGCATCTACAGTAACAAACAAATGCCGCTCCCCATGC
CGCCTTCTCTTGCAGAAATTGAACAACGTAAAGGCGTGGTGGTGATTGGCAGTGTAACACTGCAAGAAGCCAAACAGGCC
CATCAATTTGGGGCGCAAATGGGATGGCCTGTGCTGTGTGACCCACAATCCGGAACCACTTCTGATTGGAGTGGTTTTGA
TATCTGGTTGCAAAATCCTGCAGCTCGTGCACAGCTGTCCCAATGTGATTTGATCATCCAGTTTGGCCGTCGTTTGGTCT
CGAAGCGTTTACACCAATGGTTAGAGCAGCAAGTGCAAGCTGGCTGCGATTATTGGTACGTTTCACCGGATTTTGAGCGA
GACAACCAGAGCCACCTACCTCAACAGCATTTTGTCTGCTCAATCGCAGCGTGGTTGAACGTGGTGACCAACCGCGAAGT
GCAGCCTGTAGCGTGGGCGAATGAATTGCCGCGTTTTAGTGCGGAGGTGAACAAGCAAGCCAGAGAGATCGCCCAAAGCA
GCCTGTGTGAAATGATGATTGCTCTGCACTTATCCTCACTTGTGGGCAGCGCCGATCTGTTTTTAGGCAATAGCTTGTTT
GTGCGTATGGTCGATATGGTTGGTCAATTGCATGGTGTCGAAACATTTACCAATCGTGGCGCCTCGGGGATTGATGGTCT
CTTTGCCACAGCAAGTGGCGTGCAAAGGGCAAGATCCAACCCAATGTTATTGATGATTGGTGACACGTCTGCGTTGTATG
ACTTGAATTCGCTGGCGCTGTATAGCCATCAAGAGACTCCGGTGGTGATCGTGGTGACCAACAACGACGGCGGCGCGATT
TTTGATTTATTACCGGTACCACCGCAGCAAAAACAAGCGTTATACCAAATGCCTCATGGCTACCGTTTCGAATTCGCCGC
CAAACAATTTGGCCTCGATTACGTGTGCCCCACATCGATGACGGAGCTAACAGAACGAATTGTTGGCCATTTTGCACACG
GCTGTGGCGCTTTATTGGTTGAAGTCAACACGCCGCCAAACCAAGCGTCACAGCACATAAAACAATTGGCTGATCATGTC
CGTTCTCTCGTTTAA

Upstream 100 bases:

>100_bases
TTCCTGGCTCAATCGCAGAGCATGAATGGAGTGAACTGGATAAAAAAATGTCGACCTTATTGTCACTGATTTCTGAGCAC
CCACCGCTTGGAGTGGCATC

Downstream 100 bases:

>100_bases
TTATCAGCAAGGCAATGGTGATAGCCGTTCGCCCGTGTTGGTGTTACTGCACGGTTTATTGGGTAGTGCAGAAGATTGGC
GTGCCACGCAATGTTCACTC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTRLKLHTHFDERGLGFLALGLAKASQRPVAVVVT
SGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQAIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQ
QAVSGHAVHINCPFPEPLYSNAPKSIYQSYIDTVDVWRAEGGIYSNKQMPLPMPPSLAEIEQRKGVVVIGSVTLQEAKQA
HQFGAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQWLEQQVQAGCDYWYVSPDFER
DNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFSAEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLF
VRMVDMVGQLHGVETFTNRGASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVCPTSMTELTERIVGHFAHGCGALLVEVNTPPNQASQHIKQLADHV
RSLV

Sequences:

>Translated_564_residues
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTRLKLHTHFDERGLGFLALGLAKASQRPVAVVVT
SGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQAIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQ
QAVSGHAVHINCPFPEPLYSNAPKSIYQSYIDTVDVWRAEGGIYSNKQMPLPMPPSLAEIEQRKGVVVIGSVTLQEAKQA
HQFGAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQWLEQQVQAGCDYWYVSPDFER
DNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFSAEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLF
VRMVDMVGQLHGVETFTNRGASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVCPTSMTELTERIVGHFAHGCGALLVEVNTPPNQASQHIKQLADHV
RSLV
>Mature_564_residues
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTRLKLHTHFDERGLGFLALGLAKASQRPVAVVVT
SGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQAIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQ
QAVSGHAVHINCPFPEPLYSNAPKSIYQSYIDTVDVWRAEGGIYSNKQMPLPMPPSLAEIEQRKGVVVIGSVTLQEAKQA
HQFGAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQWLEQQVQAGCDYWYVSPDFER
DNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFSAEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLF
VRMVDMVGQLHGVETFTNRGASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVCPTSMTELTERIVGHFAHGCGALLVEVNTPPNQASQHIKQLADHV
RSLV

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=532, Percent_Identity=44.3609022556391, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_VIBVU (Q8D820)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761961.1
- ProteinModelPortal:   Q8D820
- GeneID:   1180000
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_3172
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   VVUL216895:VV1_3172-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 61870; Mature: 61870

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTRLKLHTHFDERGL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCCEEEEEEECCCCCCC
GFLALGLAKASQRPVAVVVTSGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQ
CEEEEHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCH
AIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQQAVSGHAVHINCPFPEPLYS
HHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHC
NAPKSIYQSYIDTVDVWRAEGGIYSNKQMPLPMPPSLAEIEQRKGVVVIGSVTLQEAKQA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHH
HQFGAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQW
HHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQQVQAGCDYWYVSPDFERDNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFS
HHHHHHCCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
AEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLFVRMVDMVGQLHGVETFTNRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCHHHHHCCC
ASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHEEEECCCCCEEEEEECCCCCEE
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVCPTSMTELTERIVGHFAHGCGALLV
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCEEEE
EVNTPPNQASQHIKQLADHVRSLV
EECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTRLKLHTHFDERGL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCCEEEEEEECCCCCCC
GFLALGLAKASQRPVAVVVTSGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQ
CEEEEHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCH
AIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQQAVSGHAVHINCPFPEPLYS
HHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHC
NAPKSIYQSYIDTVDVWRAEGGIYSNKQMPLPMPPSLAEIEQRKGVVVIGSVTLQEAKQA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHH
HQFGAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQW
HHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQQVQAGCDYWYVSPDFERDNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFS
HHHHHHCCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
AEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLFVRMVDMVGQLHGVETFTNRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCHHHHHCCC
ASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHEEEECCCCCEEEEEECCCCCEE
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVCPTSMTELTERIVGHFAHGCGALLV
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCEEEE
EVNTPPNQASQHIKQLADHVRSLV
EECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA