Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 27366396

GI number: 27366396

Start: 3200716

End: 3202686

Strand: Reverse

Name: topB [H]

Synonym: VV1_3130

Alternate gene names: 27366396

Gene position: 3202686-3200716 (Counterclockwise)

Preceding gene: 27366397

Following gene: 27366395

Centisome position: 97.59

GC content: 50.53

Gene sequence:

>1971_bases
ATGACCCGTCTGTTTATCGCTGAAAAACCCAGCTTAGGCCGCGCGATTGCTGCCGCATTGCCCAATCCTCAAAAGAAAGA
ACAAGGCTTTATTCGCTGTGGCAATGGTGACATCGTGACGTGGTGTATTGGACATTTGCTGGAGCAAGTTGAGCCAGATG
CTTACGACGAACGTTATAAAAAATGGAATATGGCGGATTTGCCCATCATCCCTGAGCAATGGCAACTGCGCCCACGTCCA
TCGGCCAGTAAACAATTGACCGTGATTCGCAAGCTGCTAAAAGAAGCAAGTGAAGTGATCCATGCAGGCGACCCTGACAG
AGAAGGGCAATTGCTGGTGGATGAAGTGCTGGATTACTGCAAACTGAGCCAAACCAAAAAAGCACAAACCCAGCGATTGT
TGATCAGCGATCTCAACCTGCCTGCAGTTAAGCGTGCGTTGAATAGTCTGCGCAGCAATCGTGATTTCATCCCACTGTCG
GTTTCTGCACTGGCACGCTCACGGGCTGATTGGCTTTATGGCATGAATATGTCACGCGCTTACACTTTGCTGGGGCAAAA
AGCAGGCTATCAAGGTGTGTTGTCAGTCGGACGAGTACAAACGCCGGTATTGGGGCTGGTGGTGCGCCGTGATGAGGAAA
TCGAGAATTTCGTTCCGCGTGATTTCTTCACTCTTCACGCGCTGATCCCTTATCAGAATGGCAGTGAACCGTTTGACATC
AAAGCACGTTGGAAACCGAGCGAAGCTTGCCAACCTTGGCAAGACGAAGAAGGGCGAGTCTTGAGTCGTAAACTGGTGGA
AAATGTTGCCAGTCGTATTGCCAATCAACCTGCTACCGTGACGGAATCGGAACACAAACAGACCAAGCAAGCCCCGCCTT
TGCCTTATTCGCTGTCGGCGCTGCAAATTGATGCGGCTAAACGCTATGGTATGAGTGCACAACAGGTGCTGGATACGTGT
CAGTCACTGTACGAAAAGCACAAACTGATCACCTACCCACGCTCGGATAACCGTTATCTTCCTAAAGAACATTATGCGCA
GGCCGCCAGTGTCAGCCAAGCCATTGCCAACAATGCGAAAGAGCTGGCCGATGCGGTGAGTGGCGCTGATCTTTCTTTAA
AATCGAAAGCGTGGAACGACAGCAAAGTCGAAGCCCACCATGCGATTATCCCCACGCCGAAACAAGCCAGTGTTAACGCG
CTGTCTGGCTACGAAATGAAGATCTATCAATTGATCGCCAGACAGTATTTGATGCAGTTCTATCCCGCTGCCACATACGC
TGAAGCCAAACTGGTGTTTGAGATTGCTGGTGGTGTCTTTATTGCGAAAGGTCGCCAATTGACGTTCGCTGGTTGGAAAG
CACTCACGGGAGCAACAGAAAAAGAAGAGGATGGGGCAGATACCGTACCACCCCTTGCGAAAGGCACTGTGCTTACCTGT
CGAGAAGGGGTAATCAAAGACTGTAAAACCGAGCCGCCAAAGCACTTTACCGAAGCGACCTTATTGCAGGCGATGACGGG
AATTGCACGTTACGTCAGTGATAAAGAACTGAAAAAAATCTTAAGAGAGACCGACGGATTAGGTACGGAAGCGACCAGGG
CGGGCATACTCGATACTCTGTTTAAACGTCAGTTACTCTCTCGACAAGGCAAAGCCATTCTTAGTACTCCAGCAGGGAGA
GGGTTGATCCATGCGTTGCCACAAGAATCAACTTATCCGGATATGACGGCTCACTGGGAGCACCAATTGCAAGGCATGGC
TGAACGCAACCAAGCCTACCAGCCATTTATGGCTGCCTTGCAACAGCGTATTTCGGAATTAATGCAGCAGGTCAAAACAT
CACCCGTGCCGAGCTCGCTGCGTCAACTGCCACCAGTGGAGCGCCCAGAGTTTAAGCGCAAGAGACGCACCAGTAGCAGC
AAGAAAACGGCGTCAACCGCGACCCAAAAAAGAACCAACAAGTCGGCTTAA

Upstream 100 bases:

>100_bases
GTTGAGTATCTATAACTGTCTATACATACAGTTTTTGTTGATTTTTAAGGGCCACATCCGCTAATGTGGCCCTTTGTTTG
TTGAGCTCGAAAAATTTGTT

Downstream 100 bases:

>100_bases
ACCCCATGATGAGCATTCCTCTGATCTATCACCCAATCTACTCTCAGTTGCCCCTGCCAGAGGGGCATCGTTACCCGATC
ATGAAATATCACCATCTTTA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MTRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRP
SASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYCKLSQTKKAQTQRLLISDLNLPAVKRALNSLRSNRDFIPLS
VSALARSRADWLYGMNMSRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSALQIDAAKRYGMSAQQVLDTC
QSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAKELADAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNA
LSGYEMKIYQLIARQYLMQFYPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTLFKRQLLSRQGKAILSTPAGR
GLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAALQQRISELMQQVKTSPVPSSLRQLPPVERPEFKRKRRTSSS
KKTASTATQKRTNKSA

Sequences:

>Translated_656_residues
MTRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRP
SASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYCKLSQTKKAQTQRLLISDLNLPAVKRALNSLRSNRDFIPLS
VSALARSRADWLYGMNMSRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSALQIDAAKRYGMSAQQVLDTC
QSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAKELADAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNA
LSGYEMKIYQLIARQYLMQFYPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTLFKRQLLSRQGKAILSTPAGR
GLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAALQQRISELMQQVKTSPVPSSLRQLPPVERPEFKRKRRTSSS
KKTASTATQKRTNKSA
>Mature_655_residues
TRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRPS
ASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYCKLSQTKKAQTQRLLISDLNLPAVKRALNSLRSNRDFIPLSV
SALARSRADWLYGMNMSRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDIK
ARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSALQIDAAKRYGMSAQQVLDTCQ
SLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAKELADAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNAL
SGYEMKIYQLIARQYLMQFYPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTCR
EGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTLFKRQLLSRQGKAILSTPAGRG
LIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAALQQRISELMQQVKTSPVPSSLRQLPPVERPEFKRKRRTSSSK
KTASTATQKRTNKSA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=624, Percent_Identity=21.7948717948718, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI10835218, Length=565, Percent_Identity=23.1858407079646, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1788061, Length=615, Percent_Identity=60.3252032520325, Blast_Score=767, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=543, Percent_Identity=26.5193370165746, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=23.6421725239617, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=632, Percent_Identity=23.5759493670886, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=522, Percent_Identity=24.3295019157088, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585251, Length=686, Percent_Identity=23.469387755102, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73285; Mature: 73154

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYK
CCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHH
KWNMADLPIIPEQWQLRPRPSASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYC
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KLSQTKKAQTQRLLISDLNLPAVKRALNSLRSNRDFIPLSVSALARSRADWLYGMNMSRA
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCHHHH
YTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHHHCCHHHHHCCCCCCEEEEEEEECCCCCCCCEE
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSA
EECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCHH
LQIDAAKRYGMSAQQVLDTCQSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAK
HEEHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELADAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNALSGYEMKIYQLIARQYLMQF
HHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
YPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
CCCCHHHHHHEEEEECCCEEEECCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEHH
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FKRQLLSRQGKAILSTPAGRGLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAAL
HHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQRISELMQQVKTSPVPSSLRQLPPVERPEFKRKRRTSSSKKTASTATQKRTNKSA
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYK
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHH
KWNMADLPIIPEQWQLRPRPSASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYC
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KLSQTKKAQTQRLLISDLNLPAVKRALNSLRSNRDFIPLSVSALARSRADWLYGMNMSRA
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCHHHH
YTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHHHCCHHHHHCCCCCCEEEEEEEECCCCCCCCEE
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSA
EECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCHH
LQIDAAKRYGMSAQQVLDTCQSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAK
HEEHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELADAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNALSGYEMKIYQLIARQYLMQF
HHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
YPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
CCCCHHHHHHEEEEECCCEEEECCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEHH
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FKRQLLSRQGKAILSTPAGRGLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAAL
HHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQRISELMQQVKTSPVPSSLRQLPPVERPEFKRKRRTSSSKKTASTATQKRTNKSA
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]