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The map label for this gene is tag [H]

Identifier: 27366152

GI number: 27366152

Start: 2939352

End: 2939921

Strand: Reverse

Name: tag [H]

Synonym: VV1_2873

Alternate gene names: 27366152

Gene position: 2939921-2939352 (Counterclockwise)

Preceding gene: 27366153

Following gene: 326424091

Centisome position: 89.58

GC content: 45.96

Gene sequence:

>570_bases
ATGATGGCAGAACAAAAAACATGTGCGTGGGCGATGAATCACCCTTTAGAACGTGAATATCATGATTCGGAATGGGGTAA
GCCTGTTTACGACGACACCGTACTTTTTGAGTTCATGACGTTAGAAGGTGCACAGGCAGGATTGAGTTGGATTACGATTC
TGAAAAAGCGTCAAGGTTATCGAGAGGCATTTGAAGGCTATGACCTCAAACGACTCGCGCAACAGGGAGAGCAAAGAGTT
GAGGAGATCATCGCTCAGTACGATGTGGTGAAACATCGTGGGAAAATTGCGTCGGTGTTTAGTAATGCACGCGCTGCATT
GGCGCTTCAAGAAGAGTTTGGTTCACTGTCTAACGCTTTGTGGTCGTTTGTCGACCATCAGCCGATTGTTAACCAATGGC
AATCAATGGATGAAGTGCCCGCGGTCACGGAGCAATCTAAAGCGCTCAGCAAGTTTCTTAAAAAGCGTGGCTTCAAGTTT
GTTGGAGAAACCATTTGTTATGCCTTCATGCAAGCGGTCGGGATGGTTGATGATCATTTAATTGATTGCCCCTGTAAAGC
ACATCGATAA

Upstream 100 bases:

>100_bases
GTTGTATCGTCTCTTCCACAAAGTGGCGGTGCCGAAATAATTAATCGGCCTAGGAATCTAAACGCCTTCACCTTGTGAGG
GCGTTTTTATAAGGAATTAG

Downstream 100 bases:

>100_bases
AAAACAGCCCCGCAGAGATGAGGGGCTGTTTTATATGCATAGCGGGGAGCGCCTAATGAGGTAAATCGCCGCTAGCTAAG
AGAGGTTTCTCTTTCAAGAA

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MMAEQKTCAWAMNHPLEREYHDSEWGKPVYDDTVLFEFMTLEGAQAGLSWITILKKRQGYREAFEGYDLKRLAQQGEQRV
EEIIAQYDVVKHRGKIASVFSNARAALALQEEFGSLSNALWSFVDHQPIVNQWQSMDEVPAVTEQSKALSKFLKKRGFKF
VGETICYAFMQAVGMVDDHLIDCPCKAHR

Sequences:

>Translated_189_residues
MMAEQKTCAWAMNHPLEREYHDSEWGKPVYDDTVLFEFMTLEGAQAGLSWITILKKRQGYREAFEGYDLKRLAQQGEQRV
EEIIAQYDVVKHRGKIASVFSNARAALALQEEFGSLSNALWSFVDHQPIVNQWQSMDEVPAVTEQSKALSKFLKKRGFKF
VGETICYAFMQAVGMVDDHLIDCPCKAHR
>Mature_189_residues
MMAEQKTCAWAMNHPLEREYHDSEWGKPVYDDTVLFEFMTLEGAQAGLSWITILKKRQGYREAFEGYDLKRLAQQGEQRV
EEIIAQYDVVKHRGKIASVFSNARAALALQEEFGSLSNALWSFVDHQPIVNQWQSMDEVPAVTEQSKALSKFLKKRGFKF
VGETICYAFMQAVGMVDDHLIDCPCKAHR

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=178, Percent_Identity=50, Blast_Score=197, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21684; Mature: 21684

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAEQKTCAWAMNHPLEREYHDSEWGKPVYDDTVLFEFMTLEGAQAGLSWITILKKRQGY
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
REAFEGYDLKRLAQQGEQRVEEIIAQYDVVKHRGKIASVFSNARAALALQEEFGSLSNAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSFVDHQPIVNQWQSMDEVPAVTEQSKALSKFLKKRGFKFVGETICYAFMQAVGMVDDHL
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IDCPCKAHR
CCCCCCCCC
>Mature Secondary Structure
MMAEQKTCAWAMNHPLEREYHDSEWGKPVYDDTVLFEFMTLEGAQAGLSWITILKKRQGY
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
REAFEGYDLKRLAQQGEQRVEEIIAQYDVVKHRGKIASVFSNARAALALQEEFGSLSNAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSFVDHQPIVNQWQSMDEVPAVTEQSKALSKFLKKRGFKFVGETICYAFMQAVGMVDDHL
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IDCPCKAHR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]