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The map label for this gene is pncB

Identifier: 27365691

GI number: 27365691

Start: 2408011

End: 2409324

Strand: Reverse

Name: pncB

Synonym: VV1_2372

Alternate gene names: 27365691

Gene position: 2409324-2408011 (Counterclockwise)

Preceding gene: 27365695

Following gene: 27365683

Centisome position: 73.41

GC content: 46.27

Gene sequence:

>1314_bases
ATGTGCGCGCCACTTTTTCAACCAGCCATTATTCAAAGTGTTCTTGATTTAGATGTGTATAAAATCAATATGATGCAAGC
CGCGTATCGATTTTATCCTCAAACACAAGTGCGCTACGAACTGATCGTTCGCTCTGATGATAACTTGTCTGATTTAGTGG
AAGAGGTTCGTGAGGAAATCAACCGCCTCGCAGAGCTTCGCTTTGATGCTGCCCAGTTGGCTTACTTGGCAGAGAAAGCG
CCCTATTTAACGGCAGAGTTTCTCTCCTATTTAGAAACCTTCCGCTTCCACCCTCAGCAACAAGTCTCTGTCGGTATTTT
CCGCACCGCACAGGGCGACTGCCAGCTCAGAGTCACCATAAATGGCATTTGGCACGAAACCATTCTCTATGAAACCTTAG
TGATGAGTATTATCTCAGAGCTGCGCAATCGTCGTTATTGGGCACAAATACCCCAGTCTCAACTGCACAAAGTATTAGAG
GACAAACTGGACTTTTTAGATTCGGAACTTAAACGCCGTAATATTACTAATTTCCGTTTCTCTGAGATGGGGACACGACG
CCGATTTAGCTTTGCTGCGCAAAAAACGATGCTTGACGTATTGCGTGCACGAGTGCCAGAACTGCTCTTAGGCACCAGTA
ATTATCACCTTGCTCAAGAGTTCAATTTAACGCCAATTGGCACGGTCGCACATGAATGGACCATGGCACATCAAGCGCTT
GTCGCTATTCAACACTCTCAGCGTGTCGCATTAGACAAATGGCTTGAGGCGTTTAATGGTAGTCTTGGTATCGCTCTAAC
CGACACCATCGGTATTGATGCGTTTCTTAGCGATTTTGACCTAGACAAAGCCACTGCCTATGCAGGCGTGAGACACGATT
CCGGCAGTCCATTTGTTTGGGGAGATAAAATCATTGCCCATTACGAAAGTCTAGGCATCGATCCAACCACTAAAACCTTG
ATTTTTACCGATGGACTGGATTTTGCGCGTGCGCTTGATATTTGCGAGTATTTCGCCGGACGCGCACAGATAAGTTTTGG
CATTGGCACTTTCCTCGCCAATGATATGGGGAATTGGACCAATGACAAAGGCACCCGTTACCAACCGATATCAATGGTGG
TCAAGATGGCGGAATGTAATGGCTCCCCAGTGGCAAAAATCAGCGACGAGCCAGAGAAAGCCATGTGTGAAGACATCTTT
TTCTTGATGAACCTCAAGCAACGCTTTGGCTTAGAAGTGGACCTTGATAAAGCGATCGAAACGCTAAAGCAGATGAAACG
TCAGCAGAAAAAGCGCATTCAAAGCGTCGCTTAA

Upstream 100 bases:

>100_bases
GATGTTAGTAACAAAAATTGACAAACCATACTCTCTCATTTAGAGTTCAGCACATTAACGAACAAAATGTTCTTAACCTA
AACAGGGTGAAAACAGACAC

Downstream 100 bases:

>100_bases
AACAAATTAGCCCCTGTCCTCTCATGGCGCCTTTCGTGGCGATTAACGACAGACACAAAAAAACCTGCCAACTGGCAGGT
TTTTTGTCTTCTATGTTTGA

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MCAPLFQPAIIQSVLDLDVYKINMMQAAYRFYPQTQVRYELIVRSDDNLSDLVEEVREEINRLAELRFDAAQLAYLAEKA
PYLTAEFLSYLETFRFHPQQQVSVGIFRTAQGDCQLRVTINGIWHETILYETLVMSIISELRNRRYWAQIPQSQLHKVLE
DKLDFLDSELKRRNITNFRFSEMGTRRRFSFAAQKTMLDVLRARVPELLLGTSNYHLAQEFNLTPIGTVAHEWTMAHQAL
VAIQHSQRVALDKWLEAFNGSLGIALTDTIGIDAFLSDFDLDKATAYAGVRHDSGSPFVWGDKIIAHYESLGIDPTTKTL
IFTDGLDFARALDICEYFAGRAQISFGIGTFLANDMGNWTNDKGTRYQPISMVVKMAECNGSPVAKISDEPEKAMCEDIF
FLMNLKQRFGLEVDLDKAIETLKQMKRQQKKRIQSVA

Sequences:

>Translated_437_residues
MCAPLFQPAIIQSVLDLDVYKINMMQAAYRFYPQTQVRYELIVRSDDNLSDLVEEVREEINRLAELRFDAAQLAYLAEKA
PYLTAEFLSYLETFRFHPQQQVSVGIFRTAQGDCQLRVTINGIWHETILYETLVMSIISELRNRRYWAQIPQSQLHKVLE
DKLDFLDSELKRRNITNFRFSEMGTRRRFSFAAQKTMLDVLRARVPELLLGTSNYHLAQEFNLTPIGTVAHEWTMAHQAL
VAIQHSQRVALDKWLEAFNGSLGIALTDTIGIDAFLSDFDLDKATAYAGVRHDSGSPFVWGDKIIAHYESLGIDPTTKTL
IFTDGLDFARALDICEYFAGRAQISFGIGTFLANDMGNWTNDKGTRYQPISMVVKMAECNGSPVAKISDEPEKAMCEDIF
FLMNLKQRFGLEVDLDKAIETLKQMKRQQKKRIQSVA
>Mature_437_residues
MCAPLFQPAIIQSVLDLDVYKINMMQAAYRFYPQTQVRYELIVRSDDNLSDLVEEVREEINRLAELRFDAAQLAYLAEKA
PYLTAEFLSYLETFRFHPQQQVSVGIFRTAQGDCQLRVTINGIWHETILYETLVMSIISELRNRRYWAQIPQSQLHKVLE
DKLDFLDSELKRRNITNFRFSEMGTRRRFSFAAQKTMLDVLRARVPELLLGTSNYHLAQEFNLTPIGTVAHEWTMAHQAL
VAIQHSQRVALDKWLEAFNGSLGIALTDTIGIDAFLSDFDLDKATAYAGVRHDSGSPFVWGDKIIAHYESLGIDPTTKTL
IFTDGLDFARALDICEYFAGRAQISFGIGTFLANDMGNWTNDKGTRYQPISMVVKMAECNGSPVAKISDEPEKAMCEDIF
FLMNLKQRFGLEVDLDKAIETLKQMKRQQKKRIQSVA

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family

Homologues:

Organism=Escherichia coli, GI1787162, Length=407, Percent_Identity=40.5405405405405, Blast_Score=310, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324783, Length=405, Percent_Identity=32.3456790123457, Blast_Score=206, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNCB_VIBVU (Q8DA38)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761219.1
- ProteinModelPortal:   Q8DA38
- SMR:   Q8DA38
- GeneID:   1179247
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2372
- HOGENOM:   HBG325503
- OMA:   GNTFYGK
- ProtClustDB:   PRK05321
- BioCyc:   VVUL216895:VV1_2372-MONOMER
- BRENDA:   2.4.2.11
- GO:   GO:0005737
- HAMAP:   MF_00570
- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638
- PANTHER:   PTHR11098:SF1
- PIRSF:   PIRSF000484
- TIGRFAMs:   TIGR01514

Pfam domain/function: PF04095 NAPRTase; SSF51690 Q_phspho_trans

EC number: =2.4.2.11

Molecular weight: Translated: 50002; Mature: 50002

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAPLFQPAIIQSVLDLDVYKINMMQAAYRFYPQTQVRYELIVRSDDNLSDLVEEVREEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHH
NRLAELRFDAAQLAYLAEKAPYLTAEFLSYLETFRFHPQQQVSVGIFRTAQGDCQLRVTI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCEEEEEEECCCCEEEEEEE
NGIWHETILYETLVMSIISELRNRRYWAQIPQSQLHKVLEDKLDFLDSELKRRNITNFRF
ECHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SEMGTRRRFSFAAQKTMLDVLRARVPELLLGTSNYHLAQEFNLTPIGTVAHEWTMAHQAL
HHHCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEHEECCCCCCHHHHHHHHHHHHHH
VAIQHSQRVALDKWLEAFNGSLGIALTDTIGIDAFLSDFDLDKATAYAGVRHDSGSPFVW
HHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHCCEECCCCCEEE
GDKIIAHYESLGIDPTTKTLIFTDGLDFARALDICEYFAGRAQISFGIGTFLANDMGNWT
CCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCC
NDKGTRYQPISMVVKMAECNGSPVAKISDEPEKAMCEDIFFLMNLKQRFGLEVDLDKAIE
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
TLKQMKRQQKKRIQSVA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCAPLFQPAIIQSVLDLDVYKINMMQAAYRFYPQTQVRYELIVRSDDNLSDLVEEVREEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHH
NRLAELRFDAAQLAYLAEKAPYLTAEFLSYLETFRFHPQQQVSVGIFRTAQGDCQLRVTI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCEEEEEEECCCCEEEEEEE
NGIWHETILYETLVMSIISELRNRRYWAQIPQSQLHKVLEDKLDFLDSELKRRNITNFRF
ECHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SEMGTRRRFSFAAQKTMLDVLRARVPELLLGTSNYHLAQEFNLTPIGTVAHEWTMAHQAL
HHHCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEHEECCCCCCHHHHHHHHHHHHHH
VAIQHSQRVALDKWLEAFNGSLGIALTDTIGIDAFLSDFDLDKATAYAGVRHDSGSPFVW
HHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHCCEECCCCCEEE
GDKIIAHYESLGIDPTTKTLIFTDGLDFARALDICEYFAGRAQISFGIGTFLANDMGNWT
CCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCC
NDKGTRYQPISMVVKMAECNGSPVAKISDEPEKAMCEDIFFLMNLKQRFGLEVDLDKAIE
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
TLKQMKRQQKKRIQSVA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA