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The map label for this gene is pilB [H]

Identifier: 27364989

GI number: 27364989

Start: 1592486

End: 1594174

Strand: Reverse

Name: pilB [H]

Synonym: VV1_1625

Alternate gene names: 27364989

Gene position: 1594174-1592486 (Counterclockwise)

Preceding gene: 27364990

Following gene: 27364988

Centisome position: 48.58

GC content: 47.78

Gene sequence:

>1689_bases
ATGATGTCGAATCTCTCCACTATTCTGCGTCAGGCCAATCAGCTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCGACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGATTACGTGAG
CTAATCACCCGTCACCAAGCGCTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAAGTGGAGATTGTTTTAGCCGATTGCCAAGAACTGCAAGCGG
CAATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGGTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATCCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCTAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTTAACCAAGAAACCGC
GATTGATATGCGTGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGAATG
ATTTTGATGACAGGCCCAACAGGAAGCGGTAAAACCGTTTCTCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATATCCACTGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCAGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTACTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Upstream 100 bases:

>100_bases
CCTCAGTCCAGTACTCGAAAAACAACACTACTGGTTGGAGCTGTGCGACAAAAAATGTACCTGCAGATTCTCGTCCAAAT
AGTTGTACTGCCACTTATTA

Downstream 100 bases:

>100_bases
TTTTTATAGTTTGTTTTTTCGATACTCATCGCACTGAAGGAAGCGATATGAAGCTTAAAACCCATTCACAAATCAAAAAC
TATCAATGGAAAGGGATTAA

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLRLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Sequences:

>Translated_562_residues
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLRLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL
>Mature_562_residues
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLRLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=485, Percent_Identity=42.4742268041237, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=48.5365853658537, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=31.2056737588652, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62479; Mature: 62479

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLRLGFFQPDELTEQLSAIFGLP
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
RTDLNQYDYQELCNQLGLRELITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQ
CCCCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCE
VEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELANLVEVSDDEVESIEDLSQDE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
SPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECEEEEEECCCHHHHHHHHHHHHH
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKL
HHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GYNPQQKQLYLDALRKPQGMILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINL
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCCEECCCCCEEEEC
PGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLH
CCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEC
TNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
CCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHH
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKD
HHHHHHHHCHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHH
SGLEKLKQGITSLEELQRVLYL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLRLGFFQPDELTEQLSAIFGLP
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
RTDLNQYDYQELCNQLGLRELITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQ
CCCCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCE
VEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELANLVEVSDDEVESIEDLSQDE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
SPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECEEEEEECCCHHHHHHHHHHHHH
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKL
HHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GYNPQQKQLYLDALRKPQGMILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINL
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCCEECCCCCEEEEC
PGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLH
CCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEC
TNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
CCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHH
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKD
HHHHHHHHCHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHH
SGLEKLKQGITSLEELQRVLYL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]