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The map label for this gene is hmp

Identifier: 27364701

GI number: 27364701

Start: 1286561

End: 1287745

Strand: Reverse

Name: hmp

Synonym: VV1_1301

Alternate gene names: 27364701

Gene position: 1287745-1286561 (Counterclockwise)

Preceding gene: 27364703

Following gene: 27364698

Centisome position: 39.24

GC content: 48.02

Gene sequence:

>1185_bases
ATGCTCAGCGAAAACACCATTAACATTGTAAAATCGACCGCCCCCCTGTTGGCCGAAACAGGACCAAAACTGACCGCCCA
TTTCTACCAACGTATGTTTGAGCACAATCCTGAACTCAAAGATATCTTTAACATGAGCAACCAACGCAATGGTGACCAAC
GAGAAGCGTTGTTCAACGCCATCTGCGCCTACGCCAGCAACATTGACAATCTGCCTGCACTCTTAGGCGCGGTAGAAAAA
ATCGCTCACAAGCACTCCAGCTTTCTGATCACTGCCGATCAATATCAAATTGTCGGCGGTCACTTGCTGGCAACCATTGA
TGAACTTTTCTCTCCGGGGCAAGCTGTGCTCGATGCCTGGGCTGAAGCTTATGGTGTGCTGGCGAATGTCTTCATTCAGC
GAGAAGAGCAAATTTATCAAGACAACCAATCGCAAACCGGCGGCTGGCGTGGCTTGCGAGAGTTTGAACTGGTCGAAAAA
CAATACGAGAGTGCGCACATCTGTAGCTTTGTCTTTAAGCCCGTCGATGGTGGCTCTGTCGTTAGCTTCAAACCAGGGCA
ATATTTAGGGATTTACATCAACGATGAGCAGTTTGAAAATCAAGAAATTCGCCAATACAGCTTGTCATCCTCTGTACGTC
CTGATTGCTATCGCATTTCAGTGAAACGTGAAGAAGGCGGCCGAGTCTCTAACTATCTGCACGATCATCTCGACGTTGGC
AGCAAAGTCAAACTCGCGGCGCCTGCCGGTGACTTTTTCCTTGATGCCGCGCCAACGGCACCAGTCGTGCTTATCTCTGC
GGGTGTCGGCCTCACGCCAACACTTTCTATGCTTGAAAGCCTAACCGAGCATCAAGCGCCGGTGACTTGGATTCATGCAA
CTGAAAATGGCCAACAACACGCATTTAAACAACACGTCAAACAATTGGTTGAAACGCATCCGCATTTCAATTCGTTAGTT
TGGTACAACCAACCAAACTCAGACGATAAAATCGGTGACGATTTCCAGTTCAGTGGATGGGTGAACCTGCATGAGATTGA
AACCGTCCTCAAACAGGCTGATGTACAAGTGTACTTCTGTGGCCCTGTTGGCTTTATGCAGTTTATAGCCAAACAATTGC
TTGAGATGGGAGTGCCTGAACAACAGTTCCATTACGAATGTTTTGGCCCACACAAAGTGGTTTAG

Upstream 100 bases:

>100_bases
AAACTTTTCTTGATTGAAAGATACATTTGAGATACAACTTTAAACATGCATTACAGATGCAACTTAAAATTAAACAAATA
ATCCTAATAGGAAGCCCGCT

Downstream 100 bases:

>100_bases
TACTAGAGTGCGACAAGGTTCGTTGAACTCAACCACACAAGACACAAAAGCCCACCGAGTTGGTGGGCTTTGGCATTCAA
AGAGAGAAGAGTGATGACAA

Product: bifunctional nitric oxide dioxygenase/dihydropteridine reductase 2

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLSENTINIVKSTAPLLAETGPKLTAHFYQRMFEHNPELKDIFNMSNQRNGDQREALFNAICAYASNIDNLPALLGAVEK
IAHKHSSFLITADQYQIVGGHLLATIDELFSPGQAVLDAWAEAYGVLANVFIQREEQIYQDNQSQTGGWRGLREFELVEK
QYESAHICSFVFKPVDGGSVVSFKPGQYLGIYINDEQFENQEIRQYSLSSSVRPDCYRISVKREEGGRVSNYLHDHLDVG
SKVKLAAPAGDFFLDAAPTAPVVLISAGVGLTPTLSMLESLTEHQAPVTWIHATENGQQHAFKQHVKQLVETHPHFNSLV
WYNQPNSDDKIGDDFQFSGWVNLHEIETVLKQADVQVYFCGPVGFMQFIAKQLLEMGVPEQQFHYECFGPHKVV

Sequences:

>Translated_394_residues
MLSENTINIVKSTAPLLAETGPKLTAHFYQRMFEHNPELKDIFNMSNQRNGDQREALFNAICAYASNIDNLPALLGAVEK
IAHKHSSFLITADQYQIVGGHLLATIDELFSPGQAVLDAWAEAYGVLANVFIQREEQIYQDNQSQTGGWRGLREFELVEK
QYESAHICSFVFKPVDGGSVVSFKPGQYLGIYINDEQFENQEIRQYSLSSSVRPDCYRISVKREEGGRVSNYLHDHLDVG
SKVKLAAPAGDFFLDAAPTAPVVLISAGVGLTPTLSMLESLTEHQAPVTWIHATENGQQHAFKQHVKQLVETHPHFNSLV
WYNQPNSDDKIGDDFQFSGWVNLHEIETVLKQADVQVYFCGPVGFMQFIAKQLLEMGVPEQQFHYECFGPHKVV
>Mature_394_residues
MLSENTINIVKSTAPLLAETGPKLTAHFYQRMFEHNPELKDIFNMSNQRNGDQREALFNAICAYASNIDNLPALLGAVEK
IAHKHSSFLITADQYQIVGGHLLATIDELFSPGQAVLDAWAEAYGVLANVFIQREEQIYQDNQSQTGGWRGLREFELVEK
QYESAHICSFVFKPVDGGSVVSFKPGQYLGIYINDEQFENQEIRQYSLSSSVRPDCYRISVKREEGGRVSNYLHDHLDVG
SKVKLAAPAGDFFLDAAPTAPVVLISAGVGLTPTLSMLESLTEHQAPVTWIHATENGQQHAFKQHVKQLVETHPHFNSLV
WYNQPNSDDKIGDDFQFSGWVNLHEIETVLKQADVQVYFCGPVGFMQFIAKQLLEMGVPEQQFHYECFGPHKVV

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=61.3636363636364, Blast_Score=509, Evalue=1e-146,
Organism=Escherichia coli, GI1787658, Length=220, Percent_Identity=26.8181818181818, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788104, Length=242, Percent_Identity=26.0330578512397, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=33.8983050847458, Blast_Score=218, Evalue=1e-57,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): HMP_VIBVU (Q8DCU2)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760229.1
- ProteinModelPortal:   Q8DCU2
- SMR:   Q8DCU2
- GeneID:   1178228
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1301
- HOGENOM:   HBG623097
- OMA:   QIGHKHR
- ProtClustDB:   PRK13289
- BioCyc:   VVUL216895:VV1_1301-MONOMER
- BRENDA:   1.14.12.17
- HAMAP:   MF_01252
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.10.490.10
- PRINTS:   PR00371
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1; SSF46458 Globin_like; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.14.12.17

Molecular weight: Translated: 44247; Mature: 44247

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS51384 FAD_FR; PS01033 GLOBIN

Important sites: ACT_SITE 95-95 ACT_SITE 135-135 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSENTINIVKSTAPLLAETGPKLTAHFYQRMFEHNPELKDIFNMSNQRNGDQREALFNA
CCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYASNIDNLPALLGAVEKIAHKHSSFLITADQYQIVGGHLLATIDELFSPGQAVLDAW
HHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCHHHHHHHHHHHCCCHHHHHHH
AEAYGVLANVFIQREEQIYQDNQSQTGGWRGLREFELVEKQYESAHICSFVFKPVDGGSV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCE
VSFKPGQYLGIYINDEQFENQEIRQYSLSSSVRPDCYRISVKREEGGRVSNYLHDHLDVG
EEECCCCEEEEEECCCHHCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
SKVKLAAPAGDFFLDAAPTAPVVLISAGVGLTPTLSMLESLTEHQAPVTWIHATENGQQH
CEEEEECCCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
AFKQHVKQLVETHPHFNSLVWYNQPNSDDKIGDDFQFSGWVNLHEIETVLKQADVQVYFC
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEECCCEEHHHHHHHHHHCCEEEEEE
GPVGFMQFIAKQLLEMGVPEQQFHYECFGPHKVV
CCHHHHHHHHHHHHHCCCCHHHCCEEECCCCCCC
>Mature Secondary Structure
MLSENTINIVKSTAPLLAETGPKLTAHFYQRMFEHNPELKDIFNMSNQRNGDQREALFNA
CCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYASNIDNLPALLGAVEKIAHKHSSFLITADQYQIVGGHLLATIDELFSPGQAVLDAW
HHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCHHHHHHHHHHHCCCHHHHHHH
AEAYGVLANVFIQREEQIYQDNQSQTGGWRGLREFELVEKQYESAHICSFVFKPVDGGSV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCE
VSFKPGQYLGIYINDEQFENQEIRQYSLSSSVRPDCYRISVKREEGGRVSNYLHDHLDVG
EEECCCCEEEEEECCCHHCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
SKVKLAAPAGDFFLDAAPTAPVVLISAGVGLTPTLSMLESLTEHQAPVTWIHATENGQQH
CEEEEECCCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
AFKQHVKQLVETHPHFNSLVWYNQPNSDDKIGDDFQFSGWVNLHEIETVLKQADVQVYFC
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEECCCEEHHHHHHHHHHCCEEEEEE
GPVGFMQFIAKQLLEMGVPEQQFHYECFGPHKVV
CCHHHHHHHHHHHHHCCCCHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA