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The map label for this gene is aspA [H]

Identifier: 27364650

GI number: 27364650

Start: 1234311

End: 1235762

Strand: Reverse

Name: aspA [H]

Synonym: VV1_1249

Alternate gene names: 27364650

Gene position: 1235762-1234311 (Counterclockwise)

Preceding gene: 27364652

Following gene: 27364649

Centisome position: 37.65

GC content: 47.38

Gene sequence:

>1452_bases
ATGGCTACCCTAACTGAAGCTCCAAAAGCATCAACTCCGGCCACTCGTATTGAAGAAGATCTATTAGGCCAACGTCACGT
TCCGGCTGACGCTTACTACGGTATTCACACGCTGCGCGCCATCGAAAACTTCAACATCTCAAATGTCACTATTTCTGACG
TACCAGAATTCGTTCGTGGCATGGTTATGACCAAAAAAGCCGCTGCGCTTGCTAACAAAGAATTGGGCGCAATTCCTAAG
AATGTCGCGGATTACATCATTCAAGCGTGTGACCTAATGTTGGAAACTGGCAAATGCATGGATCAGTTCCCATCAGACGT
ATTCCAAGGCGGTGCGGGCACTTCTGTGAACATGAACACCAACGAAGTACTTGCCAACATTGCTCTAGAATTAATGGGCA
AAGAGAAAGGCGATTACGACGTGGTGAACCCAAACGATCACGTGAACAAGAGCCAATCAACCAACTGTGCCTACCCAACG
GGCTTCCGTATTGCGGTTTACAACAGCATCCATAAATTGATGGAAGCGATTGAATACCTAAAAGGTGCGTTTGAGCTGAA
ATCTCAAGAGTTTAACGGCATCCTAAAAATGGGCCGTACTCAGTTGCAAGATGCGGTTCCAATGACAGTAGGTCAAGAGT
TTCACGCTTGGGCAGTGACGCTAAACGAAGAGATCCGTGCGCTAGACTACACCTCGAAGCTACTTCTTGAAGTGAACCTA
GGTGCAACGGCAATCGGTACTGGCTTGAACACACCTCCTGGTTACCAAGCACTTGCCGTGAAACACCTAGCGGAAGTGAC
TGGCCTAGCAGTGGTTCCAGCAGAAGACCTTATCGAAGCAACTTCAGACTGTGGTGCTTACGTAATGGCACACGGCGCTC
TAAAACGCCTAGCGGTGAAATTGTCTAAGATCTGTAACGACTTGCGTCTGCTTTCTTCTGGTCCTCGCGCTGGTTTGAAC
GAACTTAACTTGCCAGAAATGCAAGCCGGTTCTTCAATCATGCCAGCAAAAGTTAACCCAGTTATCCCAGAAGTGGTTAA
CCAAGTGTGTTTCAAAGTACTGGGTAACGACAACACGATTTCTTTCGCAGCCGAAGGCGGTCAGCTACAGCTGAACGTAA
TGGAGCCTGTGATTGGTCAAGCGATGTTTGAATCGATTTCACTACTACAAAACGCGTGTGTGAACCTACGTGACAAGTGT
ATCGACGGCATCACTGTGAACAAAGAGATCTGTGAAAACTACGTTTACAACTCTATCGGTATCGTTACTTACCTAAACCC
ATACATCGGCCACCACGAAGGTGACATCGTTGGTAAGATCTGTGCGGAAACCGGTAAGAGCGTCCGTGAAGTAGTGCTTG
AGCGTGGCTTGCTGACATCTGAAGAGTTGGATGACATCCTGTCTGTGCAGAACTTCATGCACCCAACTTACAAAGCAAAA
CGTTACGAATAA

Upstream 100 bases:

>100_bases
GACGGATTTTACAGCCAAATACTCTGATGAATGGATTCTGATTGCAGAAAATGGCTAACCAATCACTTAAATATCAGAAT
TATTATCTAAGGATCCTGTT

Downstream 100 bases:

>100_bases
TCGTAAAGAGAAAGGGCTCCTTAGCGGGCCCTTTTTGCCGTTCTTTATTAATAACCATAATAATAACAAGTATTTATTTT
ACTTCTGATTATTCAAGCGC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRGMVMTKKAAALANKELGAIPK
NVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNTNEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPT
GFRIAVYNSIHKLMEAIEYLKGAFELKSQEFNGILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
GATAIGTGLNTPPGYQALAVKHLAEVTGLAVVPAEDLIEATSDCGAYVMAHGALKRLAVKLSKICNDLRLLSSGPRAGLN
ELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTISFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKC
IDGITVNKEICENYVYNSIGIVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
RYE

Sequences:

>Translated_483_residues
MATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRGMVMTKKAAALANKELGAIPK
NVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNTNEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPT
GFRIAVYNSIHKLMEAIEYLKGAFELKSQEFNGILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
GATAIGTGLNTPPGYQALAVKHLAEVTGLAVVPAEDLIEATSDCGAYVMAHGALKRLAVKLSKICNDLRLLSSGPRAGLN
ELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTISFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKC
IDGITVNKEICENYVYNSIGIVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
RYE
>Mature_482_residues
ATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRGMVMTKKAAALANKELGAIPKN
VADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNTNEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPTG
FRIAVYNSIHKLMEAIEYLKGAFELKSQEFNGILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNLG
ATAIGTGLNTPPGYQALAVKHLAEVTGLAVVPAEDLIEATSDCGAYVMAHGALKRLAVKLSKICNDLRLLSSGPRAGLNE
LNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTISFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKCI
DGITVNKEICENYVYNSIGIVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAKR
YE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=467, Percent_Identity=39.4004282655246, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=79.2291220556745, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=450, Percent_Identity=39.5555555555556, Blast_Score=320, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17553882, Length=478, Percent_Identity=39.7489539748954, Blast_Score=343, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=39.2638036809816, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=40.5639913232104, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=40.2150537634409, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=39.6963123644252, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=39.6963123644252, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=38.8768898488121, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24583245, Length=448, Percent_Identity=34.8214285714286, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52515; Mature: 52384

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRG
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MVMTKKAAALANKELGAIPKNVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNT
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCH
NEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPTGFRIAVYNSIHKLMEAIEYL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KGAFELKSQEFNGILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEECHHHHHHHHCCEEEEEECC
GATAIGTGLNTPPGYQALAVKHLAEVTGLAVVPAEDLIEATSDCGAYVMAHGALKRLAVK
CCEEECCCCCCCCCHHHHHHHHHHHHHCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSKICNDLRLLSSGPRAGLNELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
SFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKCIDGITVNKEICENYVYNSIG
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
IVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
HHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
RYE
CCC
>Mature Secondary Structure 
ATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRG
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MVMTKKAAALANKELGAIPKNVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNT
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCH
NEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPTGFRIAVYNSIHKLMEAIEYL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KGAFELKSQEFNGILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEECHHHHHHHHCCEEEEEECC
GATAIGTGLNTPPGYQALAVKHLAEVTGLAVVPAEDLIEATSDCGAYVMAHGALKRLAVK
CCEEECCCCCCCCCHHHHHHHHHHHHHCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSKICNDLRLLSSGPRAGLNELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
SFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKCIDGITVNKEICENYVYNSIG
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
IVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
HHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
RYE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]