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The map label for this gene is dcuA [H]

Identifier: 27364649

GI number: 27364649

Start: 1232848

End: 1234155

Strand: Reverse

Name: dcuA [H]

Synonym: VV1_1248

Alternate gene names: 27364649

Gene position: 1234155-1232848 (Counterclockwise)

Preceding gene: 27364650

Following gene: 27364648

Centisome position: 37.61

GC content: 50.31

Gene sequence:

>1308_bases
ATGGTAGCGGTCGAGTTATTAGTCGTTCTGCTCTTTATCTATTTAGGGGCACGAATTGGTGGCATTGGTATTGGTTTCGC
TGGTGGTGCGGGTGTTATTGTCCTGTCGCTGATTCTAGGCGTACCAACCAAACAAGCATTTATTCCTGTTGACGTTATTT
TGATCATCATGTCTGTGATCACAGCCATTGCGGCAATGCAAGTGGCTGGTGGTATGGATTGGCTGGTTCAAATTGCTGAA
AACTTCCTGCGCAAACATCCTGAACGTATTACCTTCTACGCGCCTATCGTCACTTTCTTAATGACGTTGATGGCGGGTAC
CGGTCATACCGCATTCTCAACGCTTCCTGTGATTGCCGAAGTAGCAAAAGGGCAAGGCGTGCGTCCTTCTCGCCCATTAT
CGATTGCCGTTGTCGCATCACAAATCGCGATTACCGCTTCCCCTATTTCTGCGGCCGTGGTGGCATTTGCGGCCATGTTG
GCACCGTTTGGTGTGGACTACTTAACACTGCTTGCGGTTTGTATCCCAACCACATTTGTGGCGTGTATGATCGGCGCTTT
GGTGGCGAACTACATGGGTAGCGAGCTGAAAGACGATCCTGAGTATCAAGATCGCCTTGAAAAAGGTCTGATCAAGCTCA
GCACTGAAACACAACGTGAAATCCTGCCAACGGCTAAAACCGCGACTTACATCTTCCTTGGCGCGATTGCGTTTGTGGTT
TGCTACGCAGCGGCAATTTCCAGCTCTGTTGGTCTAATTGAAAACCCAGCGCTTGGCCGTAACGAAGCGATCATGACAGT
GATGTTGGCTGCCGCGGCAGCGATTGTGATGTTCACTAAGATCGATGCATCGAAAATCCCTTCGGCAGCGACGTTCCGTT
CAGGTATGACTGCATGTGTGTGTGTACTAGGTGTGGCTTGGTTAGGTTCTACCTTCGTTAATGCTCACGTCGATGGCATT
AAAGATGTCGCTGGTGCTTTGCTGTCTGACTACCCTTGGATGCTGGCATTGGTGCTGTTCTTTGCCTCTATGCTGCTGTA
CTCACAAGGCGCAACTACGGTTGCGCTTATGCCTGCGGCACTGGCGATTGGTGTAGCGCCACTTACTGCGGTCGCTTCTT
TTGCTGCCGTGAGTGCGCTGTTTGTTCTACCAACTTACCCAACACTATTGGCTGCCGTAGAGATGGATGATACAGGTTCA
ACCCGCATTGGTAAGTACGTGTTCAACCACCCATTCTTTATTCCGGGTGTAGCGACCATCGCTTCTGCGGTTGCGCTTGG
CTTTGCCTTCGGTAGCCTGTTTATCTAA

Upstream 100 bases:

>100_bases
CATAATAATAACAAGTATTTATTTTACTTCTGATTATTCAAGCGCTTTAGGCAAAGCGTTTCGCTAATCAGAATCAATAA
AATATATGAGGTAACAATCT

Downstream 100 bases:

>100_bases
GTAAAACGAGTCTTACTCATGGGAGCTTCGGCTCCCTTTTTTAATTTTCTGCACATAACCTTCCTATCGCTGAAACTTTC
TGCACCGTTTCTGGGTCTGA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MVAVELLVVLLFIYLGARIGGIGIGFAGGAGVIVLSLILGVPTKQAFIPVDVILIIMSVITAIAAMQVAGGMDWLVQIAE
NFLRKHPERITFYAPIVTFLMTLMAGTGHTAFSTLPVIAEVAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAML
APFGVDYLTLLAVCIPTTFVACMIGALVANYMGSELKDDPEYQDRLEKGLIKLSTETQREILPTAKTATYIFLGAIAFVV
CYAAAISSSVGLIENPALGRNEAIMTVMLAAAAAIVMFTKIDASKIPSAATFRSGMTACVCVLGVAWLGSTFVNAHVDGI
KDVAGALLSDYPWMLALVLFFASMLLYSQGATTVALMPAALAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGS
TRIGKYVFNHPFFIPGVATIASAVALGFAFGSLFI

Sequences:

>Translated_435_residues
MVAVELLVVLLFIYLGARIGGIGIGFAGGAGVIVLSLILGVPTKQAFIPVDVILIIMSVITAIAAMQVAGGMDWLVQIAE
NFLRKHPERITFYAPIVTFLMTLMAGTGHTAFSTLPVIAEVAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAML
APFGVDYLTLLAVCIPTTFVACMIGALVANYMGSELKDDPEYQDRLEKGLIKLSTETQREILPTAKTATYIFLGAIAFVV
CYAAAISSSVGLIENPALGRNEAIMTVMLAAAAAIVMFTKIDASKIPSAATFRSGMTACVCVLGVAWLGSTFVNAHVDGI
KDVAGALLSDYPWMLALVLFFASMLLYSQGATTVALMPAALAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGS
TRIGKYVFNHPFFIPGVATIASAVALGFAFGSLFI
>Mature_435_residues
MVAVELLVVLLFIYLGARIGGIGIGFAGGAGVIVLSLILGVPTKQAFIPVDVILIIMSVITAIAAMQVAGGMDWLVQIAE
NFLRKHPERITFYAPIVTFLMTLMAGTGHTAFSTLPVIAEVAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAML
APFGVDYLTLLAVCIPTTFVACMIGALVANYMGSELKDDPEYQDRLEKGLIKLSTETQREILPTAKTATYIFLGAIAFVV
CYAAAISSSVGLIENPALGRNEAIMTVMLAAAAAIVMFTKIDASKIPSAATFRSGMTACVCVLGVAWLGSTFVNAHVDGI
KDVAGALLSDYPWMLALVLFFASMLLYSQGATTVALMPAALAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGS
TRIGKYVFNHPFFIPGVATIASAVALGFAFGSLFI

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=436, Percent_Identity=57.5688073394495, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1790565, Length=397, Percent_Identity=36.7758186397985, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45273; Mature: 45273

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVELLVVLLFIYLGARIGGIGIGFAGGAGVIVLSLILGVPTKQAFIPVDVILIIMSVI
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TAIAAMQVAGGMDWLVQIAENFLRKHPERITFYAPIVTFLMTLMAGTGHTAFSTLPVIAE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAMLAPFGVDYLTLLAVCIPTTFV
HHCCCCCCCCCCEEHEEEHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ACMIGALVANYMGSELKDDPEYQDRLEKGLIKLSTETQREILPTAKTATYIFLGAIAFVV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEECCCHHHHHHCCCHHHHHHHHHHHHHHHH
CYAAAISSSVGLIENPALGRNEAIMTVMLAAAAAIVMFTKIDASKIPSAATFRSGMTACV
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHH
CVLGVAWLGSTFVNAHVDGIKDVAGALLSDYPWMLALVLFFASMLLYSQGATTVALMPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKYVFNHPFFIPGVATI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCHHHHHHHCCCCCCCCHHHH
ASAVALGFAFGSLFI
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVAVELLVVLLFIYLGARIGGIGIGFAGGAGVIVLSLILGVPTKQAFIPVDVILIIMSVI
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TAIAAMQVAGGMDWLVQIAENFLRKHPERITFYAPIVTFLMTLMAGTGHTAFSTLPVIAE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAMLAPFGVDYLTLLAVCIPTTFV
HHCCCCCCCCCCEEHEEEHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ACMIGALVANYMGSELKDDPEYQDRLEKGLIKLSTETQREILPTAKTATYIFLGAIAFVV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEECCCHHHHHHCCCHHHHHHHHHHHHHHHH
CYAAAISSSVGLIENPALGRNEAIMTVMLAAAAAIVMFTKIDASKIPSAATFRSGMTACV
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHH
CVLGVAWLGSTFVNAHVDGIKDVAGALLSDYPWMLALVLFFASMLLYSQGATTVALMPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKYVFNHPFFIPGVATI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCHHHHHHHCCCCCCCCHHHH
ASAVALGFAFGSLFI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]