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The map label for this gene is uvrD [H]

Identifier: 27364389

GI number: 27364389

Start: 955030

End: 957204

Strand: Reverse

Name: uvrD [H]

Synonym: VV1_0949

Alternate gene names: 27364389

Gene position: 957204-955030 (Counterclockwise)

Preceding gene: 27364394

Following gene: 27364385

Centisome position: 29.17

GC content: 51.68

Gene sequence:

>2175_bases
ATGATCGATCCTTCACTTCTTCTCGATGGCTTGAACGACAAACAGCGTGAGGCTGTTGCTGCACCTTTGGAAAACTTGCT
GGTGTTGGCAGGCGCAGGCAGTGGTAAAACGCGCGTTCTTGTGCATCGCATTGCTTGGCTAATGTCAGTCGAGCAGGCTT
CTCCGTTTTCTATTATGTCTGTGACCTTTACCAATAAGGCGGCGGCGGAGATGCGTGGTCGAATCGAAGAGTTGATGATG
GGCAGTGCGTCGGGCATGTGGAATGGCACCTTCCACGGTATTTGCCACCGAATTCTCAGAGCGCACTATCTGGATGCCAA
GCTGCCGGAAGATTTCCAAATTATTGATAGCGACGACCAACAACGTTTGCTTAAGCGACTGATCAAAGCGCAAAACCTGG
ATGACAAACAGTGGCCAGCGCGCCAAGTCGCTTGGTGGATCAATGGCAAAAAAGACGAAGGATTACGCCCTGCGCACATC
GATGCCTACCACGATCCCGTGACCAAAACCTACCTACAGTTGTACACCGCCTATCAAGAAGCGTGTGATCGCGCAGGCTT
GGTCGACTTTGCGGAGATCTTGCTGCGTGCGCATGAGCTACTGCGTGAGAACAAATTTATCCGCGAACACTATCAAGCGC
GCTTTAAACACATCTTGGTGGACGAGTTCCAAGACACCAACAACATTCAATACGCTTGGCTGCGTATGATGGCGGGGCCT
GAATGCCATGTGATGATCGTCGGTGATGATGACCAGTCGATTTACGGTTGGCGTGGCGCGAAAGTGGAAAACATCGAAAA
GTTCACCCTTGAGTTTCCGAGCGTCAACACCATTCGCCTCGAGCAAAACTACCGTTCGACCAAAACCATCCTAGAAGCCT
CAAACGCCTTGATTGCCAACAATACCGAGCGTATGGGCAAAGAGCTGTGGACCGATGGCGTCGAAGGTGAACCGATCTCG
ATTTATTCTGCTTACAACGAATTGGATGAAGCGCGTTTTGCGGTGAACAAAATCAAAGAGTGGCAGGACAAAGGCGGCGT
GTTGAACGACGCAGCGATGCTTTATCGCAATAACGCCCAGTCGCGTGTACTGGAAGAAGCGTTGATTCAAGCGGGTTTGC
CTTATCGCATTTACGGCGGGATGCGATTCTTCGAGCGCCAAGAGATCAAAGATGCATTGAGCTATCTGCGCCTCATCGCC
AACCGCAATGACGACGCGGCCTTTGAACGTGTGGTTAATACTCCAACGCGTGGCTTAGGGGACAAAACCTTGGAAACGGT
GCGTTTTGCCGCGCGCGATCGTGGCTGCACCTTGTGGGAAGCCAGTATCGCTTTGATCGAAGAAAAAGTGTTAACGGGGC
GTGCTGCTGGTGCGTTAAGTCGCTTTATCGAGCTGATCACGGCGCTGGAAGACGACAGCTTTGAAATGCCACTGCACATT
CAAACCGACCATGTGATCAAATCCTCAGGCTTGTACACCATGTATGAGCAAGAGAAAGGCGAGAAGTCCAAGGCTCGCAT
TGAGAACTTGGAAGAGTTGGTCACGGCGACACGTCAGTTTGAAAAACCGGAAGAAGCCGAAGAGATGAGTTTGCTCACTG
CTTTCTTAACCCATGCCGCTTTGGAGGCGGGTGAAGGTCAAGCGGACGATTTCGAAGATGCGGTACAACTGATGACCTTA
CACAGCGCCAAAGGATTGGAGTTCCCATTGGTGTTTATGGTGGGCGTGGAAGAAGGCATGTTCCCGAGCCAGATGTCCGC
CGAAGAAGCGGGCCGCTTAGAAGAAGAGCGCCGTTTGTGCTACGTGGGCATGACTCGGGCAATGCAGAAGCTCTATATTA
CCTACGCTGAAATGCGTCGTTTGTACGGGCAAGACAAATATCATAAACCATCACGTTTTATCCGAGAGCTGCCGGAAAGC
TGCTTGGATGAAGTGCGCATGAAAGCGCAAGTCAGCCGTCCTGCCAGCAGTGGTCGCTTTAGCCAAACTGTGGTCAAAGA
GAGCTTCAATGAAACGGGCTTTTCGCTCGGCTCTCGTGTGCGTCACCCAAAGTTTGGTGAAGGGACGATCATCAACTTTG
AAGGCAGTGGGCCACAAAGTCGAGTTCAAGTGGCCTTCAATGGGGAAGGAATCAAGTGGCTAGTGACCGCTTACGCGAGG
CTGGAAAAACTGTGA

Upstream 100 bases:

>100_bases
TGGTTGATGCCTTTTTCTTAAATTGCAATTTAGTCTGAAAAGCGAGCACGGTTTAGGTTATAATCCTTGTTTCTGTATAA
ATAACCAGTAGCTTCTTATC

Downstream 100 bases:

>100_bases
TGGTTTATCGATCGATCTTCGATCTGCAAACGGCGCTGAAGGCGCCGTTTTTTATGTCTCAGTGAGCGGGGACAAATAGC
AAGCAAAGAAAAACCCCGAG

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MIDPSLLLDGLNDKQREAVAAPLENLLVLAGAGSGKTRVLVHRIAWLMSVEQASPFSIMSVTFTNKAAAEMRGRIEELMM
GSASGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQQRLLKRLIKAQNLDDKQWPARQVAWWINGKKDEGLRPAHI
DAYHDPVTKTYLQLYTAYQEACDRAGLVDFAEILLRAHELLRENKFIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
ECHVMIVGDDDQSIYGWRGAKVENIEKFTLEFPSVNTIRLEQNYRSTKTILEASNALIANNTERMGKELWTDGVEGEPIS
IYSAYNELDEARFAVNKIKEWQDKGGVLNDAAMLYRNNAQSRVLEEALIQAGLPYRIYGGMRFFERQEIKDALSYLRLIA
NRNDDAAFERVVNTPTRGLGDKTLETVRFAARDRGCTLWEASIALIEEKVLTGRAAGALSRFIELITALEDDSFEMPLHI
QTDHVIKSSGLYTMYEQEKGEKSKARIENLEELVTATRQFEKPEEAEEMSLLTAFLTHAALEAGEGQADDFEDAVQLMTL
HSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPES
CLDEVRMKAQVSRPASSGRFSQTVVKESFNETGFSLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
LEKL

Sequences:

>Translated_724_residues
MIDPSLLLDGLNDKQREAVAAPLENLLVLAGAGSGKTRVLVHRIAWLMSVEQASPFSIMSVTFTNKAAAEMRGRIEELMM
GSASGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQQRLLKRLIKAQNLDDKQWPARQVAWWINGKKDEGLRPAHI
DAYHDPVTKTYLQLYTAYQEACDRAGLVDFAEILLRAHELLRENKFIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
ECHVMIVGDDDQSIYGWRGAKVENIEKFTLEFPSVNTIRLEQNYRSTKTILEASNALIANNTERMGKELWTDGVEGEPIS
IYSAYNELDEARFAVNKIKEWQDKGGVLNDAAMLYRNNAQSRVLEEALIQAGLPYRIYGGMRFFERQEIKDALSYLRLIA
NRNDDAAFERVVNTPTRGLGDKTLETVRFAARDRGCTLWEASIALIEEKVLTGRAAGALSRFIELITALEDDSFEMPLHI
QTDHVIKSSGLYTMYEQEKGEKSKARIENLEELVTATRQFEKPEEAEEMSLLTAFLTHAALEAGEGQADDFEDAVQLMTL
HSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPES
CLDEVRMKAQVSRPASSGRFSQTVVKESFNETGFSLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
LEKL
>Mature_724_residues
MIDPSLLLDGLNDKQREAVAAPLENLLVLAGAGSGKTRVLVHRIAWLMSVEQASPFSIMSVTFTNKAAAEMRGRIEELMM
GSASGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQQRLLKRLIKAQNLDDKQWPARQVAWWINGKKDEGLRPAHI
DAYHDPVTKTYLQLYTAYQEACDRAGLVDFAEILLRAHELLRENKFIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
ECHVMIVGDDDQSIYGWRGAKVENIEKFTLEFPSVNTIRLEQNYRSTKTILEASNALIANNTERMGKELWTDGVEGEPIS
IYSAYNELDEARFAVNKIKEWQDKGGVLNDAAMLYRNNAQSRVLEEALIQAGLPYRIYGGMRFFERQEIKDALSYLRLIA
NRNDDAAFERVVNTPTRGLGDKTLETVRFAARDRGCTLWEASIALIEEKVLTGRAAGALSRFIELITALEDDSFEMPLHI
QTDHVIKSSGLYTMYEQEKGEKSKARIENLEELVTATRQFEKPEEAEEMSLLTAFLTHAALEAGEGQADDFEDAVQLMTL
HSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPES
CLDEVRMKAQVSRPASSGRFSQTVVKESFNETGFSLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
LEKL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=723, Percent_Identity=72.1991701244813, Blast_Score=1092, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=642, Percent_Identity=38.785046728972, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=29.1176470588235, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=736, Percent_Identity=27.5815217391304, Blast_Score=195, Evalue=2e-50,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82339; Mature: 82339

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPSLLLDGLNDKQREAVAAPLENLLVLAGAGSGKTRVLVHRIAWLMSVEQASPFSIMS
CCCHHHHHCCCCHHHHHHHHHCHHCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VTFTNKAAAEMRGRIEELMMGSASGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQ
EEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCH
QRLLKRLIKAQNLDDKQWPARQVAWWINGKKDEGLRPAHIDAYHDPVTKTYLQLYTAYQE
HHHHHHHHHHCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ACDRAGLVDFAEILLRAHELLRENKFIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
ECHVMIVGDDDQSIYGWRGAKVENIEKFTLEFPSVNTIRLEQNYRSTKTILEASNALIAN
CEEEEEEECCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCEEC
NTERMGKELWTDGVEGEPISIYSAYNELDEARFAVNKIKEWQDKGGVLNDAAMLYRNNAQ
CHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
SRVLEEALIQAGLPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGLG
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DKTLETVRFAARDRGCTLWEASIALIEEKVLTGRAAGALSRFIELITALEDDSFEMPLHI
HHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
QTDHVIKSSGLYTMYEQEKGEKSKARIENLEELVTATRQFEKPEEAEEMSLLTAFLTHAA
CCCCEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LEAGEGQADDFEDAVQLMTLHSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLC
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCHHHHHHHH
YVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPESCLDEVRMKAQVSRPASSGRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SQTVVKESFNETGFSLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
LEKL
HHCC
>Mature Secondary Structure
MIDPSLLLDGLNDKQREAVAAPLENLLVLAGAGSGKTRVLVHRIAWLMSVEQASPFSIMS
CCCHHHHHCCCCHHHHHHHHHCHHCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VTFTNKAAAEMRGRIEELMMGSASGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQ
EEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCH
QRLLKRLIKAQNLDDKQWPARQVAWWINGKKDEGLRPAHIDAYHDPVTKTYLQLYTAYQE
HHHHHHHHHHCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ACDRAGLVDFAEILLRAHELLRENKFIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
ECHVMIVGDDDQSIYGWRGAKVENIEKFTLEFPSVNTIRLEQNYRSTKTILEASNALIAN
CEEEEEEECCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCEEC
NTERMGKELWTDGVEGEPISIYSAYNELDEARFAVNKIKEWQDKGGVLNDAAMLYRNNAQ
CHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
SRVLEEALIQAGLPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGLG
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DKTLETVRFAARDRGCTLWEASIALIEEKVLTGRAAGALSRFIELITALEDDSFEMPLHI
HHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
QTDHVIKSSGLYTMYEQEKGEKSKARIENLEELVTATRQFEKPEEAEEMSLLTAFLTHAA
CCCCEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LEAGEGQADDFEDAVQLMTLHSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLC
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCHHHHHHHH
YVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPESCLDEVRMKAQVSRPASSGRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SQTVVKESFNETGFSLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
LEKL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]