Click here to switch to the map view.

The map label for this gene is aceA [H]

Identifier: 27363917

GI number: 27363917

Start: 445307

End: 446620

Strand: Reverse

Name: aceA [H]

Synonym: VV1_0449

Alternate gene names: 27363917

Gene position: 446620-445307 (Counterclockwise)

Preceding gene: 27363918

Following gene: 326423713

Centisome position: 13.61

GC content: 49.85

Gene sequence:

>1314_bases
ATGACAAACTTAACTCGCCGCCAGCAAATCGAAGCTCTGGAAAAAGATTGGGCAACCAATCCTCGTTGGAAAAACGTCAA
ACGCACTTACACAGCTGAGGAAGTGGTGGCCTTACGTGGCTCTATGGTGCCAGCTAATACCATCGCGCAGCGTGGTGCCG
ACAAGTTGTGGTCGTTGGTGAATGGTAGCTCGAAAAAAGGCTATGTGAATTGTCTAGGCGCACTAACCGGTGGCCAAGCG
GTGCAGCAAGCCAAAGCGGGGATAGAAGCGATCTACCTATCGGGTTGGCAGGTTGCAGCGGATAACAACACCGCCTCGAC
CATGTACCCAGACCAATCACTCTACCCTGTGGATTCGGTTCCTTCAGTCGTCAAACGCATTAACAATTCGTTCCGTCGCG
CAGACCAAATTCAATGGTCGAATGGTAAGTCGCCAGAAGAGGGTGGAATTGATTACTTCTTGCCTATCGTTGCTGATGCA
GAAGCGGGCTTTGGTGGTGTGCTGAATGCGTATGAACTGATGAAGTCGATGATTGAAGCGGGGGCTGCGGGTGTACACTT
TGAAGACCAATTGGCTTCGGTGAAGAAGTGTGGCCATATGGGCGGTAAAGTATTGGTTCCAACGCAAGAGGCGGTACAAA
AACTGGTTGCTGCGCGTCTAGCCGCAGATGTTGCTGGCACAACAACACTGGTGATTGCTCGAACCGATGCCAATGCGGCG
GACTTGTTGACGTCAGATTGTGACCCATATGACGCAGACTTTATTGTTGGCGAGCGCACTCAGGAAGGTTTCTATCGTGT
TCGTGCAGGGATTGACCAAGCGATCTCACGTGGCCTTGCTTATGCGCCATATGCGGATCTTATTTGGTGTGAAACGGCAA
CTCCGTGTCTTGAAGAAGCACGTAAGTTTGCTGAGGCTATTCATGCGGAATACCCAGACCAACTGTTGGCATACAACTGC
TCGCCATCATTTAACTGGGAGAAGAACTTGGATGCAGAGACCATTGCTAAGTTCCAACAAGAACTCGCAGATATGGGCTA
TAAGTACCAGTTCATCACCCTAGCTGGTATTCACAACATGTGGTTCAACATGTTTGAACTGGCACACGCATATGCTCAAG
GTGAAGGTATGCGTCACTACGTTGAGAAAGTTCAGCGTCCTGAATTTGAAGCGGCGGAAAAAGGCTACACCTTCGTTGCA
CACCAACAAGAAGTAGGTACGGGTTACTTTGATAAGATGACCAACACCATCCAAGGTGGTAACTCTTCAGTCACGGCACT
GACGGGCTCTACCGAAGAAGACCAGTTCCATTAA

Upstream 100 bases:

>100_bases
GTGAACCTTCATTGGCGGAGCAGGATGTACCGCTAACGAAATGGAATATATAGCGCACTCAATTTTAATAATCAGCGCTC
ATCAAAAGAGGGATAGACCG

Downstream 100 bases:

>100_bases
TACCTTTCCATCTTTAGGTATTAACCACTGCAAGAGCGGCGAAAGCCGCTCTTTTTCCTTTCCTATCAGGACAAACAAGG
ATCTCGCAGCTAAGAGTGTC

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLVNGSSKKGYVNCLGALTGGQA
VQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADA
EAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDANAA
DLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEARKFAEAIHAEYPDQLLAYNC
SPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVA
HQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH

Sequences:

>Translated_437_residues
MTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLVNGSSKKGYVNCLGALTGGQA
VQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADA
EAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDANAA
DLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEARKFAEAIHAEYPDQLLAYNC
SPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVA
HQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH
>Mature_436_residues
TNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLVNGSSKKGYVNCLGALTGGQAV
QQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADAE
AGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDANAAD
LLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEARKFAEAIHAEYPDQLLAYNCS
PSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVAH
QQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=77.5981524249423, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=268, Percent_Identity=29.1044776119403, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982926, Length=419, Percent_Identity=57.0405727923628, Blast_Score=472, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17561814, Length=419, Percent_Identity=57.0405727923628, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320908, Length=290, Percent_Identity=38.2758620689655, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6325263, Length=249, Percent_Identity=37.7510040160643, Blast_Score=162, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48033; Mature: 47902

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLV
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSV
CCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCHHH
PSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDANAA
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
DLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEA
HHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHH
RKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNM
HHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHH
WFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQGG
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEEEHHHHCCCHHHHHHHHHCCC
NSSVTALTGSTEEDQFH
CCEEEEEECCCCCCCCC
>Mature Secondary Structure 
TNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLV
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSV
CCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCHHH
PSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDANAA
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
DLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEA
HHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHH
RKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNM
HHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHH
WFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQGG
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEEEHHHHCCCHHHHHHHHHCCC
NSSVTALTGSTEEDQFH
CCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]