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The map label for this gene is alaS [C]

Identifier: 27363890

GI number: 27363890

Start: 418170

End: 419054

Strand: Reverse

Name: alaS [C]

Synonym: VV1_0422

Alternate gene names: 27363890

Gene position: 419054-418170 (Counterclockwise)

Preceding gene: 27363891

Following gene: 27363889

Centisome position: 12.77

GC content: 47.68

Gene sequence:

>885_bases
ATGCAGATAACACCCACTAAGGTTCGCTTTTGTCATCAGACCTGGCAGTTGGAGAGTGACATTGTACTCACTTCAGCCAA
TGAACATTCTCTTTATGTCGTGACTCAGCACACTCCATTTCATCCGGTCAGTCATATTTGGCCAGATCATCCTGCAGATC
GGGGAAGTCTTATCGCGGATGGCAAAGAGTGGCCCATCATCGGTTGTTGGGTTGGCGCTGTAGAGTGCTCTACAGGGCGC
CTGTTTGTTGATCAAGCGATACCTGTAAAGCGAGATACAGAGGGATGGAGCTTCGTAGTGGTTCATGAAATTGAAGCCGA
GCGTGGGCAACTTTCTGTTGGAAGCAAAGTACGATTGAGTGTCGACAAAGAATATCAACAAAGCCTGAGTCGAGGTCACA
GTGCAGGGCATTTAGCCTATCTGGCATTGAATAAAGTCTTGGCGCAAGGTTACTGGCGCAAAGATGCCGATCGCAAAGAC
CCTCATGGTTACTATGATTTTAACAGTTATGCTCAAGAGAGTAGCGTTGTGACGCCAGAGTGTTGTCTAGACACCTATCG
ACTGGGAAAAACGCTACGCAAAAGAGGGCTGAACAGTGCTGATGTGGTGGAAAATATTCAAGAGATTGAATCCGAGGTCA
ATGTACAGCTTGAGCACTGGCTTGCTCGCGGATCCGAAATATTCATGAACTGTCATGGCGAATATTTAACCGACTCTCGT
TACTGGCAATGTGATTTGGGAGAAGCCTCAACGGCGATTATCCCTTGTGGGGGAACGCATGCTGCTGGGCTGTTCGAAAT
GAAAGAGATTGCCGTTACATTGGTGCTGTTGGATGAGCAGACCATTGAAATGCACACGCAAGTCACGCCCAACAGAGAAA
AATGA

Upstream 100 bases:

>100_bases
AAGTTTTACTTGGGCATTTTTTTATTTTCCGTTTCCCAATATGGATAACATGCCACATCATTCGTTGAGCGGATGATGCC
TCTTTGTTGAGATAAGAAAT

Downstream 100 bases:

>100_bases
TCTTTATATAAAAAACCAAAAATACAGATTCAAATATGCCTATATGGATTTTTATGTTGTTTAAAATTGAAATGCAGCAT
ATGTAACTTATGCTTTCGGG

Product: putative metal-dependent hydrolase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MQITPTKVRFCHQTWQLESDIVLTSANEHSLYVVTQHTPFHPVSHIWPDHPADRGSLIADGKEWPIIGCWVGAVECSTGR
LFVDQAIPVKRDTEGWSFVVVHEIEAERGQLSVGSKVRLSVDKEYQQSLSRGHSAGHLAYLALNKVLAQGYWRKDADRKD
PHGYYDFNSYAQESSVVTPECCLDTYRLGKTLRKRGLNSADVVENIQEIESEVNVQLEHWLARGSEIFMNCHGEYLTDSR
YWQCDLGEASTAIIPCGGTHAAGLFEMKEIAVTLVLLDEQTIEMHTQVTPNREK

Sequences:

>Translated_294_residues
MQITPTKVRFCHQTWQLESDIVLTSANEHSLYVVTQHTPFHPVSHIWPDHPADRGSLIADGKEWPIIGCWVGAVECSTGR
LFVDQAIPVKRDTEGWSFVVVHEIEAERGQLSVGSKVRLSVDKEYQQSLSRGHSAGHLAYLALNKVLAQGYWRKDADRKD
PHGYYDFNSYAQESSVVTPECCLDTYRLGKTLRKRGLNSADVVENIQEIESEVNVQLEHWLARGSEIFMNCHGEYLTDSR
YWQCDLGEASTAIIPCGGTHAAGLFEMKEIAVTLVLLDEQTIEMHTQVTPNREK
>Mature_294_residues
MQITPTKVRFCHQTWQLESDIVLTSANEHSLYVVTQHTPFHPVSHIWPDHPADRGSLIADGKEWPIIGCWVGAVECSTGR
LFVDQAIPVKRDTEGWSFVVVHEIEAERGQLSVGSKVRLSVDKEYQQSLSRGHSAGHLAYLALNKVLAQGYWRKDADRKD
PHGYYDFNSYAQESSVVTPECCLDTYRLGKTLRKRGLNSADVVENIQEIESEVNVQLEHWLARGSEIFMNCHGEYLTDSR
YWQCDLGEASTAIIPCGGTHAAGLFEMKEIAVTLVLLDEQTIEMHTQVTPNREK

Specific function: Unknown

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 33196; Mature: 33196

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITPTKVRFCHQTWQLESDIVLTSANEHSLYVVTQHTPFHPVSHIWPDHPADRGSLIAD
CCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCCHHHCCCCCCCCCCCEEEC
GKEWPIIGCWVGAVECSTGRLFVDQAIPVKRDTEGWSFVVVHEIEAERGQLSVGSKVRLS
CCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEE
VDKEYQQSLSRGHSAGHLAYLALNKVLAQGYWRKDADRKDPHGYYDFNSYAQESSVVTPE
ECHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHH
CCLDTYRLGKTLRKRGLNSADVVENIQEIESEVNVQLEHWLARGSEIFMNCHGEYLTDSR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEHHHHHHCCCEEEEEECCCEECCCC
YWQCDLGEASTAIIPCGGTHAAGLFEMKEIAVTLVLLDEQTIEMHTQVTPNREK
EEEEECCCCCEEEEECCCCCHHHHHHHHHEEEEEEEECCCEEEEEEECCCCCCC
>Mature Secondary Structure
MQITPTKVRFCHQTWQLESDIVLTSANEHSLYVVTQHTPFHPVSHIWPDHPADRGSLIAD
CCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCCHHHCCCCCCCCCCCEEEC
GKEWPIIGCWVGAVECSTGRLFVDQAIPVKRDTEGWSFVVVHEIEAERGQLSVGSKVRLS
CCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEE
VDKEYQQSLSRGHSAGHLAYLALNKVLAQGYWRKDADRKDPHGYYDFNSYAQESSVVTPE
ECHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHH
CCLDTYRLGKTLRKRGLNSADVVENIQEIESEVNVQLEHWLARGSEIFMNCHGEYLTDSR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEHHHHHHCCCEEEEEECCCEECCCC
YWQCDLGEASTAIIPCGGTHAAGLFEMKEIAVTLVLLDEQTIEMHTQVTPNREK
EEEEECCCCCEEEEECCCCCHHHHHHHHHEEEEEEEECCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA