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The map label for this gene is hlyB [H]

Identifier: 27363527

GI number: 27363527

Start: 44889

End: 46523

Strand: Reverse

Name: hlyB [H]

Synonym: VV1_0040

Alternate gene names: 27363527

Gene position: 46523-44889 (Counterclockwise)

Preceding gene: 27363528

Following gene: 27363526

Centisome position: 1.42

GC content: 50.03

Gene sequence:

>1635_bases
ATGTTCAATCGACTCTCAATTAAACAGAAGCTGATGCTGCCCATTGGCTTACTGACATTGGTGATCGTTGCGACGACACT
GATCAATGTGCTGCAAGCCAATAAACAAGCTGAGCTTAATTCAATGCTCAATACTCAAGTGCAGCCGGTGATGGATAGCT
TAGAAGATGGTTATCGAGACATTTACCAAGTGATCACAGCTGCTCAAGGAATATTACTGGCGCAGTCACAAAGCGAGGTG
GATTATCACATTGGTGAGTTTAAAGATAATGCCTACAACGTCGAACCGAGAGTTGGCAAAGTCCGAGAGCTGTTTAATGC
CGGTTTGTTGCCAGAATCGGAGCGTCAGCAACTGACGAATCTAACGAGTGCCGCAAAAGCGTGGTTAGCGCTTTATGAGC
CGATGTTTGCCCAGCCAAAAAATGCGGCGGGTTACTACCAAGATAACCGTCGCCAGTTTGAGCAGCAATTTGCCGTATTG
CGCCAGCAACTGGTTTCCATTCGCAGCTTAATCGAGCAAGTGCAAGGGCAACTAAGAGAAGAGGTTGAACGTTCGATTGA
TTACAGTGTGTATGTGATTGAAGCTGGGGCGGTTATCTCCATCGCATTGGTGCTGCTCTCTTTCGTCATGGCCAAGCAAT
TAATTCTTAAACCCATAGAAGGCATTAGTCACGCCATGGCGGAAATCGCGTCAGGGGATGGCGATCTCTCAAAACGCATG
GTGGCGACCAGTGACGACGAACTCGGACTACTGGCAGGCGCATTTAATCAATTTGTTTCTCGGATCCACTCCACAGTCGA
GCAGGTGATTATTTCCTCCAACGCGGTTCGCGCTGAAATGGAGAACATCAAAACGCTGACGCAAACGGTGGCCGAGTTCT
CAGGCCAGCAGCAACAAGAAAGTGAGATGGTGGCCACCGCGGTTAACGAAATGCAGATGACCAGCCAGTCGGTTGCCGAG
AATGCCAATGAAGCGGCGTCGGCCAGTCATAAAGCCAACCGCGAAGCGGACAACACCAACGCCATTTTGAAGACCACCGT
CAGCTCTATTCAAAGTCTCGCCGGGGAAGTGGATAACGCCAGCCAAGTCATTCACAACCTGGATCTCGATGTAGCGAACA
TTGCCTCTATTCTTGATGTGATTCGAGGCATTGCCGATCAAACCAATCTGCTGGCGCTAAACGCAGCGATCGAAGCGGCT
CGCGCCGGTGAGCAAGGTCGTGGCTTTGCCGTGGTGGCCGATGAAGTGCGTTCGCTCGCTAGCCGTACTCAAGAAAGCAC
GGGTGAAATTCAATCGATGATCGAAAAACTGCAACAAGGGGCGCGAGAAGCGGTTGCGGTGATGCAAGCGAGTAAAGAGC
GTGGCCACGATACGATTGATAATGCAGGTAAAGCCAGTGATTCGCTGGCAGAAATCATCGGATCGATTTCGTTGATGAGC
CAAATGAACTCACAAATTGCGACCGCCGCCAGCGAGCAAACCAGTGTCAGCGATGAGGTCAACAACAATGTGATGCACAT
TTTGGAAAACAGTCATCACATGGTGGAAATGGTCAGCAGTGCAGAAAATGCTTGTCTATCGTTGGCTGGTCAGTGCGAAA
CATTGGACGACTTGGTCGCGCAGTTCAAAGTTTAG

Upstream 100 bases:

>100_bases
TAATTCATACCCCAAGTAAGCCTTGTTCTCAAAACCTCTAGCCAAGGCGCTAGAGTGTCATTGAGAATGGGCGATCAATC
AGCGATGAATAAGGCCATTT

Downstream 100 bases:

>100_bases
AGCCCCTGATTTCTCTCGGTGTTTGTCGAGAGAAGTCTGTGGTAGCGTAGCGAAAAACTCATCAGGAAGAATGCGCTATG
TCTCTTTGGTCGTCGGTGAA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MFNRLSIKQKLMLPIGLLTLVIVATTLINVLQANKQAELNSMLNTQVQPVMDSLEDGYRDIYQVITAAQGILLAQSQSEV
DYHIGEFKDNAYNVEPRVGKVRELFNAGLLPESERQQLTNLTSAAKAWLALYEPMFAQPKNAAGYYQDNRRQFEQQFAVL
RQQLVSIRSLIEQVQGQLREEVERSIDYSVYVIEAGAVISIALVLLSFVMAKQLILKPIEGISHAMAEIASGDGDLSKRM
VATSDDELGLLAGAFNQFVSRIHSTVEQVIISSNAVRAEMENIKTLTQTVAEFSGQQQQESEMVATAVNEMQMTSQSVAE
NANEAASASHKANREADNTNAILKTTVSSIQSLAGEVDNASQVIHNLDLDVANIASILDVIRGIADQTNLLALNAAIEAA
RAGEQGRGFAVVADEVRSLASRTQESTGEIQSMIEKLQQGAREAVAVMQASKERGHDTIDNAGKASDSLAEIIGSISLMS
QMNSQIATAASEQTSVSDEVNNNVMHILENSHHMVEMVSSAENACLSLAGQCETLDDLVAQFKV

Sequences:

>Translated_544_residues
MFNRLSIKQKLMLPIGLLTLVIVATTLINVLQANKQAELNSMLNTQVQPVMDSLEDGYRDIYQVITAAQGILLAQSQSEV
DYHIGEFKDNAYNVEPRVGKVRELFNAGLLPESERQQLTNLTSAAKAWLALYEPMFAQPKNAAGYYQDNRRQFEQQFAVL
RQQLVSIRSLIEQVQGQLREEVERSIDYSVYVIEAGAVISIALVLLSFVMAKQLILKPIEGISHAMAEIASGDGDLSKRM
VATSDDELGLLAGAFNQFVSRIHSTVEQVIISSNAVRAEMENIKTLTQTVAEFSGQQQQESEMVATAVNEMQMTSQSVAE
NANEAASASHKANREADNTNAILKTTVSSIQSLAGEVDNASQVIHNLDLDVANIASILDVIRGIADQTNLLALNAAIEAA
RAGEQGRGFAVVADEVRSLASRTQESTGEIQSMIEKLQQGAREAVAVMQASKERGHDTIDNAGKASDSLAEIIGSISLMS
QMNSQIATAASEQTSVSDEVNNNVMHILENSHHMVEMVSSAENACLSLAGQCETLDDLVAQFKV
>Mature_544_residues
MFNRLSIKQKLMLPIGLLTLVIVATTLINVLQANKQAELNSMLNTQVQPVMDSLEDGYRDIYQVITAAQGILLAQSQSEV
DYHIGEFKDNAYNVEPRVGKVRELFNAGLLPESERQQLTNLTSAAKAWLALYEPMFAQPKNAAGYYQDNRRQFEQQFAVL
RQQLVSIRSLIEQVQGQLREEVERSIDYSVYVIEAGAVISIALVLLSFVMAKQLILKPIEGISHAMAEIASGDGDLSKRM
VATSDDELGLLAGAFNQFVSRIHSTVEQVIISSNAVRAEMENIKTLTQTVAEFSGQQQQESEMVATAVNEMQMTSQSVAE
NANEAASASHKANREADNTNAILKTTVSSIQSLAGEVDNASQVIHNLDLDVANIASILDVIRGIADQTNLLALNAAIEAA
RAGEQGRGFAVVADEVRSLASRTQESTGEIQSMIEKLQQGAREAVAVMQASKERGHDTIDNAGKASDSLAEIIGSISLMS
QMNSQIATAASEQTSVSDEVNNNVMHILENSHHMVEMVSSAENACLSLAGQCETLDDLVAQFKV

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=367, Percent_Identity=29.9727520435967, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787690, Length=437, Percent_Identity=27.2311212814645, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788195, Length=288, Percent_Identity=32.6388888888889, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788194, Length=360, Percent_Identity=28.6111111111111, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789453, Length=311, Percent_Identity=31.1897106109325, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 59086; Mature: 59086

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNRLSIKQKLMLPIGLLTLVIVATTLINVLQANKQAELNSMLNTQVQPVMDSLEDGYRD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYQVITAAQGILLAQSQSEVDYHIGEFKDNAYNVEPRVGKVRELFNAGLLPESERQQLTN
HHHHHHHHCCHHEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
LTSAAKAWLALYEPMFAQPKNAAGYYQDNRRQFEQQFAVLRQQLVSIRSLIEQVQGQLRE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVERSIDYSVYVIEAGAVISIALVLLSFVMAKQLILKPIEGISHAMAEIASGDGDLSKRM
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VATSDDELGLLAGAFNQFVSRIHSTVEQVIISSNAVRAEMENIKTLTQTVAEFSGQQQQE
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SEMVATAVNEMQMTSQSVAENANEAASASHKANREADNTNAILKTTVSSIQSLAGEVDNA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
SQVIHNLDLDVANIASILDVIRGIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLA
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
SRTQESTGEIQSMIEKLQQGAREAVAVMQASKERGHDTIDNAGKASDSLAEIIGSISLMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHH
QMNSQIATAASEQTSVSDEVNNNVMHILENSHHMVEMVSSAENACLSLAGQCETLDDLVA
HHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QFKV
HHCC
>Mature Secondary Structure
MFNRLSIKQKLMLPIGLLTLVIVATTLINVLQANKQAELNSMLNTQVQPVMDSLEDGYRD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYQVITAAQGILLAQSQSEVDYHIGEFKDNAYNVEPRVGKVRELFNAGLLPESERQQLTN
HHHHHHHHCCHHEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
LTSAAKAWLALYEPMFAQPKNAAGYYQDNRRQFEQQFAVLRQQLVSIRSLIEQVQGQLRE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVERSIDYSVYVIEAGAVISIALVLLSFVMAKQLILKPIEGISHAMAEIASGDGDLSKRM
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VATSDDELGLLAGAFNQFVSRIHSTVEQVIISSNAVRAEMENIKTLTQTVAEFSGQQQQE
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SEMVATAVNEMQMTSQSVAENANEAASASHKANREADNTNAILKTTVSSIQSLAGEVDNA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
SQVIHNLDLDVANIASILDVIRGIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLA
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
SRTQESTGEIQSMIEKLQQGAREAVAVMQASKERGHDTIDNAGKASDSLAEIIGSISLMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHH
QMNSQIATAASEQTSVSDEVNNNVMHILENSHHMVEMVSSAENACLSLAGQCETLDDLVA
HHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QFKV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]