BacMap

Definition	Burkholderia mallei NCTC 10247 chromosome II, complete genome.
Accession	NC_009079
Length	2,352,693

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The map label for this gene is fadB1 [H]

Identifier: 262192876

GI number: 262192876

Start: 1831366

End: 1832142

Strand: Reverse

Name: fadB1 [H]

Synonym: BMA10247_A1899

Alternate gene names: 262192876

Gene position: 1832142-1831366 (Counterclockwise)

Preceding gene: 126447439

Following gene: 126447210

Centisome position: 77.87

GC content: 63.06

Gene sequence:

>777_bases
ATGGCTTACGAGAACATTCTGGTGGAGACGCGGGGCAGGGTGGGGCTGATTACGCTGAACCGTCCGAAGGCGCTGAATGC
GTTGAATGACGCCCTGATGGATGAACTTGGCTTGGCGCTCAAAGCGTTCGACGCGGACGAGGGTATTGGCGCGATCGTGC
TGACGGGCAGCGAGAAGGCGTTCGCGGCGGGCGCCGACATCGGGATGATGGCGACCTATTCATACATGGACGTCTTCAAG
GGCGACTACATCACGCGTAACTGGGAGACGGTGCGCCAGATCCGCAAGCCGATCATCGCGGCGGTGGCGGGTTTCGCGCT
GGGCGGCGGCTGCGAACTCGCGATGATGTGCGACATCATCTTTGCGGCCGATACGGCGAAGTTCGGCCAGCCGGAAATCA
AGCTCGGCGTGCTGCCGGGTGCGGGCGGCACGCAGCGCCTGCCGCGCGCGGTGTCGAAGGCGAAAGCGATGGACATGTGC
TTGACCGCGCGCTTCATGGACGCGGAGGAGGCTGAGCGGGCGGGCTTGGTGTCGAGGATCCTGCCGGCGGACAAGTTGCT
CGACGAGGCGATCGCCGCGGCGACGACGATCGCGGAATTCTCGCTGCCTGCCGTAATGATGGTGAAGGAGGCGGTGAATC
GCGCATACGAGACGACCTTGTCCGAAGGGGTGCACTTCGAGCGTCGCTTGTTTCACTCGGCATTTGCGACTGAAGATCAG
AAGGAAGGGATGGCGGCGTTCGTCGAGAAGCGCAAGCCCGTCTTCAAGCATCGCTGA

Upstream 100 bases:

>100_bases
CGGTGGCCATTAATTAACCGACCGGTTAGTCGGAAAATGTTAGCATCAAATCATCCGTGCGTGTGCGGCATTTCATGTTT
CATTGATCGAGGAGAAGCAG

Downstream 100 bases:

>100_bases
ACACGCACAGGGCAAAGTGCTGCTTGCCCTTGCCTCGAATTTCCGGTCTGCCGATGCTTGAATTCGAGGCGTGAAAAATT
CTTTCAAAAAACGCTTGCAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYENILVETRGRVGLITLNRPKALNALNDALMDELGLALKAFDADEGIGAIVLTGSEKAFAAGADIGMMATYSYMDVFK
GDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAEEAERAGLVSRILPADKLLDEAIAAATTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
KEGMAAFVEKRKPVFKHR

Sequences:

>Translated_258_residues
MAYENILVETRGRVGLITLNRPKALNALNDALMDELGLALKAFDADEGIGAIVLTGSEKAFAAGADIGMMATYSYMDVFK
GDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAEEAERAGLVSRILPADKLLDEAIAAATTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
KEGMAAFVEKRKPVFKHR
>Mature_257_residues
AYENILVETRGRVGLITLNRPKALNALNDALMDELGLALKAFDADEGIGAIVLTGSEKAFAAGADIGMMATYSYMDVFKG
DYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMCL
TARFMDAEEAERAGLVSRILPADKLLDEAIAAATTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQK
EGMAAFVEKRKPVFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=56.7567567567568, Blast_Score=295, Evalue=2e-80,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=36.71875, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=31.8352059925094, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI68989263, Length=230, Percent_Identity=32.6086956521739, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=37.2881355932203, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=32.3770491803279, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI37594469, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.124513618677, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.124513618677, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=26.3829787234043, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=26.3829787234043, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=23.828125, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=23.828125, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=26.0663507109005, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=26.0663507109005, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136756, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136753, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221307494, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=32.5925925925926, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=213, Percent_Identity=25.8215962441315, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=44.6640316205534, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.1538461538462, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=34.0996168582375, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=34.7490347490347, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=32.0754716981132, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=30.8080808080808, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=56.5384615384615, Blast_Score=294, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=54.7325102880658, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=34.6456692913386, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=32.7800829875519, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=30.9090909090909, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=34.6153846153846, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=34.6153846153846, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=34.6153846153846, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.5625, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=30.0751879699248, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=34.0782122905028, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=220, Percent_Identity=29.5454545454545, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=220, Percent_Identity=29.5454545454545, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=24.7104247104247, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=28.4974093264249, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=58.9147286821705, Blast_Score=304, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=58.9147286821705, Blast_Score=304, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=37.218045112782, Blast_Score=150, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=32.6923076923077, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650670, Length=256, Percent_Identity=30.859375, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=32.5490196078431, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=27.6679841897233, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=30.4812834224599, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921000, Length=170, Percent_Identity=35.2941176470588, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=34.4827586206897, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=34.4827586206897, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571730, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27945; Mature: 27814

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYENILVETRGRVGLITLNRPKALNALNDALMDELGLALKAFDADEGIGAIVLTGSEKA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVFKGDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMCLTARFMDAEEAERAGLVSRI
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LPADKLLDEAIAAATTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYENILVETRGRVGLITLNRPKALNALNDALMDELGLALKAFDADEGIGAIVLTGSEKA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVFKGDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVLPGAGGTQRLPRAVSKAKAMDMCLTARFMDAEEAERAGLVSRI
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LPADKLLDEAIAAATTIAEFSLPAVMMVKEAVNRAYETTLSEGVHFERRLFHSAFATEDQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]