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The map label for this gene is metG [H]

Identifier: 258676992

GI number: 258676992

Start: 645687

End: 646037

Strand: Direct

Name: metG [H]

Synonym: CLC_0629

Alternate gene names: 258676992

Gene position: 645687-646037 (Clockwise)

Preceding gene: 153936415

Following gene: 153934577

Centisome position: 17.17

GC content: 33.33

Gene sequence:

>351_bases
ATGGTTGCTCCAGTTAAAGCAAATATCAATATGGATGTACTTGACAAGATTGATATTAGAGTGGGAACAATTAAATTAGT
TGAAGATGTTGAGAAATCAGATAAATTAGTTAAGTTATCTGTTGATTTTGGTGGATTTACCAGAACTATACTAGTAGGGA
TGAAGGGTGAAAGAGATAATCCTAAAGAAATTGAAGGTAAACAGGCATTATTTGTAGTTAATTTGGCTCCAAAAAAAATG
GCAGGAGAAGTTTCTGAAGGGATGCTTTTTGATATAGGATATGCTGATGGTATTATACCTGTTTTAGCACAACCTGAAAA
ATCAATACCTAATGGTACAAGGGTTGGGTAA

Upstream 100 bases:

>100_bases
ATAAATTATAATGAATAAAGAAAAAGTAAATTTAATAAAAGATAGAAGAATTTGTATTATGGTATAATTAGCTAAAATAG
GATATAAAGGAGGAAATGAT

Downstream 100 bases:

>100_bases
TACTTAATAATATGTTCATTGGTATTTTTATTGGTATAGGAATTGTTCCTACGATTATGATAATTATTGAAATGATTAGC
TATTTTTATTATGATAAGAG

Product: tRNA-binding domain protein

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MVAPVKANINMDVLDKIDIRVGTIKLVEDVEKSDKLVKLSVDFGGFTRTILVGMKGERDNPKEIEGKQALFVVNLAPKKM
AGEVSEGMLFDIGYADGIIPVLAQPEKSIPNGTRVG

Sequences:

>Translated_116_residues
MVAPVKANINMDVLDKIDIRVGTIKLVEDVEKSDKLVKLSVDFGGFTRTILVGMKGERDNPKEIEGKQALFVVNLAPKKM
AGEVSEGMLFDIGYADGIIPVLAQPEKSIPNGTRVG
>Mature_116_residues
MVAPVKANINMDVLDKIDIRVGTIKLVEDVEKSDKLVKLSVDFGGFTRTILVGMKGERDNPKEIEGKQALFVVNLAPKKM
AGEVSEGMLFDIGYADGIIPVLAQPEKSIPNGTRVG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788432, Length=90, Percent_Identity=38.8888888888889, Blast_Score=76, Evalue=5e-16,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 12540; Mature: 12540

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPVKANINMDVLDKIDIRVGTIKLVEDVEKSDKLVKLSVDFGGFTRTILVGMKGERDN
CCCCEECCCCEEEEEEEEEEEEEEEEEHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCC
PKEIEGKQALFVVNLAPKKMAGEVSEGMLFDIGYADGIIPVLAQPEKSIPNGTRVG
CCCCCCCEEEEEEEECCCHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MVAPVKANINMDVLDKIDIRVGTIKLVEDVEKSDKLVKLSVDFGGFTRTILVGMKGERDN
CCCCEECCCCEEEEEEEEEEEEEEEEEHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCC
PKEIEGKQALFVVNLAPKKMAGEVSEGMLFDIGYADGIIPVLAQPEKSIPNGTRVG
CCCCCCCEEEEEEEECCCHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA