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The map label for this gene is manZ [H]

Identifier: 258676989

GI number: 258676989

Start: 2872357

End: 2873346

Strand: Direct

Name: manZ [H]

Synonym: CLC_2721

Alternate gene names: 258676989

Gene position: 2872357-2873346 (Clockwise)

Preceding gene: 153936130

Following gene: 153937260

Centisome position: 76.38

GC content: 34.65

Gene sequence:

>990_bases
GTGGTGTTGTTTTCTTGTAATTCTTTTTCTTCTTACGACAAACAGATTTTAAATTCAGAATTGACAAAGGACATGAAAAT
GGCAAAAGAATTAAAATTAACCAAAAAAGATCATATTTCTGTTTGGTTGCGTTCATTTTTCCTTCAAGGTTCTTGGAACT
ATGAAAGAATGCAAAACGGTGGTTGGGCATTCGCAATGATTCCTGCAATCAAAAAATTATACAAGACTAAAGAAGATCGA
GCAGATGCATTGAAACGTCACTTGGAGTTCTTTAACACTCACCCATATGTAGCTTCACCAGTTCTTGGGGTAACATTAGC
TTTAGAAGAACAACGTGCAAATGGTGCAGAAATCGATGATATAACTATTCAAGGTGTTAAAGTTGGTATGATGGGACCTT
TAGCTGGTATTGGAGATCCAGTTTTTTGGTTTACTGTTAGGTCAATTTTAGGGGCATTAGCTTCTTCACTTGCTATGAAT
GGTAATATCCTAGGACCATTAATCTTTTTCTTGGCTTGGAATATCATCCGTATGGGATTAATGTGGTATACACAAGAGTT
CGGTTTCAAAACAGAATCTTGTATTGCTGATGATTTATCAGGTGGTTTACTACAAGATATTACAAAAGGAGCATCCATCC
TTGGTATGTTCATTTTAGGAGCATTAGTTAACAGATGGGTATCCGTTAAATTTACACCAGTAGTATCATCCGTTAAGTTA
AGTGATGGTGCTTTCATTGATTGGAGCAAACTTCCTAAAGGAGTGAAAGGTATTCAACAAGTTCTTTTACAACAAGGATC
TGGTATGTCATTAACTGATTATAAAGTTACAACATTCCAAGATAGCTTGGATTCATTAATTCCTGGACTTGCAGAATTAA
TAATTACATTTATTTGTATGTGGTTACTTAAGAAGAAAGTATCTCCAATTACCATTATCCTTGGGATATTTGCAATGGGT
ATTGTTTTCCACTTAATCGGTTTAATGTAA

Upstream 100 bases:

>100_bases
CCTCCAATTCCGTACTTCATCTCAGTTACATCTGTAAGCCATTTCTCCCCGAATTTATCAGAATTAAAATTCCTATTTAA
AGTATTTTCTGCTGTTACTT

Downstream 100 bases:

>100_bases
CATTTTTTATTTAGCCTAGGCTTTTAGCCTGGGCTTTTGTTAGCATATAAAGCAGTTAAAGAATGAAACAAATTGATGAA
ATATGTGTTATTTTTAATGG

Product: PTS system mannose/fructose/sorbose family IID subunit

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MVLFSCNSFSSYDKQILNSELTKDMKMAKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDR
ADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMN
GNILGPLIFFLAWNIIRMGLMWYTQEFGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKL
SDGAFIDWSKLPKGVKGIQQVLLQQGSGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMG
IVFHLIGLM

Sequences:

>Translated_329_residues
MVLFSCNSFSSYDKQILNSELTKDMKMAKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDR
ADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMN
GNILGPLIFFLAWNIIRMGLMWYTQEFGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKL
SDGAFIDWSKLPKGVKGIQQVLLQQGSGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMG
IVFHLIGLM
>Mature_329_residues
MVLFSCNSFSSYDKQILNSELTKDMKMAKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDR
ADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMN
GNILGPLIFFLAWNIIRMGLMWYTQEFGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKL
SDGAFIDWSKLPKGVKGIQQVLLQQGSGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMG
IVFHLIGLM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=312, Percent_Identity=48.3974358974359, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1789529, Length=303, Percent_Identity=32.3432343234323, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 36526; Mature: 36526

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLFSCNSFSSYDKQILNSELTKDMKMAKELKLTKKDHISVWLRSFFLQGSWNYERMQNG
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
GWAFAMIPAIKKLYKTKEDRADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDD
CEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHCHHHHHHEEEEHHCCCCCCCCC
ITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMNGNILGPLIFFLAWNIIRMGL
EEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MWYTQEFGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKL
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEEEE
SDGAFIDWSKLPKGVKGIQQVLLQQGSGMSLTDYKVTTFQDSLDSLIPGLAELIITFICM
CCCCEEEHHHCCHHHHHHHHHHHHCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHH
WLLKKKVSPITIILGIFAMGIVFHLIGLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVLFSCNSFSSYDKQILNSELTKDMKMAKELKLTKKDHISVWLRSFFLQGSWNYERMQNG
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
GWAFAMIPAIKKLYKTKEDRADALKRHLEFFNTHPYVASPVLGVTLALEEQRANGAEIDD
CEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHCHHHHHHEEEEHHCCCCCCCCC
ITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMNGNILGPLIFFLAWNIIRMGL
EEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MWYTQEFGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKL
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEEEE
SDGAFIDWSKLPKGVKGIQQVLLQQGSGMSLTDYKVTTFQDSLDSLIPGLAELIITFICM
CCCCEEEHHHCCHHHHHHHHHHHHCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHH
WLLKKKVSPITIILGIFAMGIVFHLIGLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]