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The map label for this gene is yxiM

Identifier: 255767829

GI number: 255767829

Start: 4017508

End: 4018656

Strand: Reverse

Name: yxiM

Synonym: BSU39120

Alternate gene names: 255767829

Gene position: 4018656-4017508 (Counterclockwise)

Preceding gene: 255767830

Following gene: 255767828

Centisome position: 95.33

GC content: 50.3

Gene sequence:

>1149_bases
ATGAAAAAATGGATGGCAGCGGTTTTTGTGATGATGCTGATGCTGTGTTTTGGCGGGATTGAGAATGTGAAGGCGGCGGA
GCCGAAGGTGTATCAGTTTGACTTTGGAAGCGGTTCGATGGAGCCTGGTTATATTGGTGTCAGGGCGTCTGATCGGTATG
ACCGGTCAAAGGGCTACGGTTTTCAAACACCGGAGAATATGAGGGATGTGGCGGCATCCGGGGCTGGTGTGAAGAGTGAT
GCGGTTCAGTTTTTAGCGTATGGGACGAAAAGCAATAACACGTTTAATGTTGATCTCCCGAATGGCCTTTATGAGGTGAA
GGTGACGCTTGGCAATACGGCAAGGGCCAGTGTGGCAGCGGAGGGCGTGTTTCAGGTCATCAATATGACAGGGGATGGCG
CGGAGGATACGTTCCAAATTCCCGTCACCGACGGGCAGCTGAATCTGCTGGTGACAGAGGGAAAGGCAGGCACCGCTTTT
ACGCTCAGCGCCTTGAAAATAAAGAAATTGTCTGATCAGCCGGTAACGAATCGAACCATTTATGTCGGCGGCGACTCGAC
GGTGTGCAATTATTATCCGCTCAACAGCAGCAAGCAGGCGGGCTGGGGGCAGATGCTGCCTCACTATATCGATAAACACA
CCTTTCAAGTGAGAAACATGGCGTCTGGCGGGCAGATCGCGAGAGGGTTCAGAAATGATGGACAGCTTGAGGCGATTCTG
AAGTATATTAAACCCGGAGATTATTTTATGTTGCAGCTTGGCATTAATGACACAAATCCGAAGCATAAAGAATCTGAAGC
GGAGTTTAAAGAGGTGATGCGTGATATGATTCGTCAGGTAAAAGCGAAAGGAGCGGACGTCATCCTATCAACGCCTCAGG
GCCGGGCAACCGATTTTACTTCTGAAGGCATCCATTCGTCTGTAAACAGATGGTACAGGGCCTCTATTTTAGCTTTGGCC
GAAGAGGAAAAAACATATCTCATTGACTTAAATGTCCTCAGCTCGGCATACTTTACATCGATCGGTCCGGAAAGAACACT
CGGGCTTTATATGGATGGAGATACGCTGCACCCGAATCGCGCGGGGGCCGACGCACTGGCGCGATTGGCTGTTCAGGAGC
TAAAACGCCAGGGAATCGCTGGCTTTTAA

Upstream 100 bases:

>100_bases
TTTTATTCCTCAACGCAATTTTTTTCTTAATTCAGTCATCATAAAGAAACAATCACCGAGTATGCATTAATGTATTCGTT
TTCATAGAGGGGGAGTCTTG

Downstream 100 bases:

>100_bases
TACATCATTTTTCGACAAAACACATATGCCTTGGTCAATTAGAGTATAATGAGAACATCTGCAAGAGACAGGAGTTTATA
CATGAGTCATTTTAAAAACT

Product: esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKKWMAAVFVMMLMLCFGGIENVKAAEPKVYQFDFGSGSMEPGYIGVRASDRYDRSKGYGFQTPENMRDVAASGAGVKSD
AVQFLAYGTKSNNTFNVDLPNGLYEVKVTLGNTARASVAAEGVFQVINMTGDGAEDTFQIPVTDGQLNLLVTEGKAGTAF
TLSALKIKKLSDQPVTNRTIYVGGDSTVCNYYPLNSSKQAGWGQMLPHYIDKHTFQVRNMASGGQIARGFRNDGQLEAIL
KYIKPGDYFMLQLGINDTNPKHKESEAEFKEVMRDMIRQVKAKGADVILSTPQGRATDFTSEGIHSSVNRWYRASILALA
EEEKTYLIDLNVLSSAYFTSIGPERTLGLYMDGDTLHPNRAGADALARLAVQELKRQGIAGF

Sequences:

>Translated_382_residues
MKKWMAAVFVMMLMLCFGGIENVKAAEPKVYQFDFGSGSMEPGYIGVRASDRYDRSKGYGFQTPENMRDVAASGAGVKSD
AVQFLAYGTKSNNTFNVDLPNGLYEVKVTLGNTARASVAAEGVFQVINMTGDGAEDTFQIPVTDGQLNLLVTEGKAGTAF
TLSALKIKKLSDQPVTNRTIYVGGDSTVCNYYPLNSSKQAGWGQMLPHYIDKHTFQVRNMASGGQIARGFRNDGQLEAIL
KYIKPGDYFMLQLGINDTNPKHKESEAEFKEVMRDMIRQVKAKGADVILSTPQGRATDFTSEGIHSSVNRWYRASILALA
EEEKTYLIDLNVLSSAYFTSIGPERTLGLYMDGDTLHPNRAGADALARLAVQELKRQGIAGF
>Mature_382_residues
MKKWMAAVFVMMLMLCFGGIENVKAAEPKVYQFDFGSGSMEPGYIGVRASDRYDRSKGYGFQTPENMRDVAASGAGVKSD
AVQFLAYGTKSNNTFNVDLPNGLYEVKVTLGNTARASVAAEGVFQVINMTGDGAEDTFQIPVTDGQLNLLVTEGKAGTAF
TLSALKIKKLSDQPVTNRTIYVGGDSTVCNYYPLNSSKQAGWGQMLPHYIDKHTFQVRNMASGGQIARGFRNDGQLEAIL
KYIKPGDYFMLQLGINDTNPKHKESEAEFKEVMRDMIRQVKAKGADVILSTPQGRATDFTSEGIHSSVNRWYRASILALA
EEEKTYLIDLNVLSSAYFTSIGPERTLGLYMDGDTLHPNRAGADALARLAVQELKRQGIAGF

Specific function: Unknown

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YXIM_BACSU (P42304)

Other databases:

- EMBL:   D83026
- EMBL:   AL009126
- PIR:   A70078
- RefSeq:   NP_391791.2
- PDB:   2O14
- PDBsum:   2O14
- ProteinModelPortal:   P42304
- SMR:   P42304
- EnsemblBacteria:   EBBACT00000003391
- GeneID:   937485
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39120
- NMPDR:   fig|224308.1.peg.3917
- GenoList:   BSU39120
- GeneTree:   EBGT00050000006183
- HOGENOM:   HBG322748
- ProtClustDB:   CLSK626751
- BioCyc:   BSUB:BSU39120-MONOMER
- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- Gene3D:   G3DSA:3.40.50.1110

Pfam domain/function: PF00657 Lipase_GDSL; SSF52266 Esterase_SGNH_hydro-type

EC number: NA

Molecular weight: Translated: 41836; Mature: 41836

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: ACT_SITE 186-186 ACT_SITE 354-354 ACT_SITE 357-357

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWMAAVFVMMLMLCFGGIENVKAAEPKVYQFDFGSGSMEPGYIGVRASDRYDRSKGYG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCC
FQTPENMRDVAASGAGVKSDAVQFLAYGTKSNNTFNVDLPNGLYEVKVTLGNTARASVAA
CCCCHHHHHHHHCCCCCCHHHEEEEEECCCCCCEEEEECCCCEEEEEEEECCCCHHHHHH
EGVFQVINMTGDGAEDTFQIPVTDGQLNLLVTEGKAGTAFTLSALKIKKLSDQPVTNRTI
HHHHHEEECCCCCCCCEEEEEEECCEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCEE
YVGGDSTVCNYYPLNSSKQAGWGQMLPHYIDKHTFQVRNMASGGQIARGFRNDGQLEAIL
EECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCHHHHHH
KYIKPGDYFMLQLGINDTNPKHKESEAEFKEVMRDMIRQVKAKGADVILSTPQGRATDFT
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
SEGIHSSVNRWYRASILALAEEEKTYLIDLNVLSSAYFTSIGPERTLGLYMDGDTLHPNR
HHHHHHHHHHHHHHHHEEEECCCCEEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
AGADALARLAVQELKRQGIAGF
CCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKWMAAVFVMMLMLCFGGIENVKAAEPKVYQFDFGSGSMEPGYIGVRASDRYDRSKGYG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCC
FQTPENMRDVAASGAGVKSDAVQFLAYGTKSNNTFNVDLPNGLYEVKVTLGNTARASVAA
CCCCHHHHHHHHCCCCCCHHHEEEEEECCCCCCEEEEECCCCEEEEEEEECCCCHHHHHH
EGVFQVINMTGDGAEDTFQIPVTDGQLNLLVTEGKAGTAFTLSALKIKKLSDQPVTNRTI
HHHHHEEECCCCCCCCEEEEEEECCEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCEE
YVGGDSTVCNYYPLNSSKQAGWGQMLPHYIDKHTFQVRNMASGGQIARGFRNDGQLEAIL
EECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCHHHHHH
KYIKPGDYFMLQLGINDTNPKHKESEAEFKEVMRDMIRQVKAKGADVILSTPQGRATDFT
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
SEGIHSSVNRWYRASILALAEEEKTYLIDLNVLSSAYFTSIGPERTLGLYMDGDTLHPNR
HHHHHHHHHHHHHHHHEEEECCCCEEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
AGADALARLAVQELKRQGIAGF
CCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377