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The map label for this gene is yugT

Identifier: 255767708

GI number: 255767708

Start: 3214372

End: 3216036

Strand: Reverse

Name: yugT

Synonym: BSU31290

Alternate gene names: 255767708

Gene position: 3216036-3214372 (Counterclockwise)

Preceding gene: 255767709

Following gene: 255767707

Centisome position: 76.29

GC content: 43.6

Gene sequence:

>1665_bases
ATGAAAAAGGCTTGGTGGAAGGAAGCCGTAGTTTACCAAATTTACCCCCGCAGTTTTAAAGATTCAAATGGCGACGGAAT
CGGGGATATTCAAGGAATCAGAACCAAGCTTTCTTACATAAAAGAGCTGGGCGCTGACGTCATATGGATTTGCCCGCTGT
ATGATTCACCGAATGCCGATAATGGCTATGATATCAGGGATTATCAAAACATATTAAGCGAGTTTGGCACGATGGAAGAT
TTTGATGAGCTGCTTGGTGACATTCATGATCTGGATATGAAGCTGATTATGGATCTCGTTGTGAACCACACAAGCGACGA
ACATCCATGGTTCATAGAATCCCGTTCATCCATACACTCGGAAAAGCGCGACTGGTATATTTGGAAAGACGGCAAAAACG
GAAAAACGCCCAACAATTGGGAAAGCATTTTCGGCGGGCCGGCTTGGGAGTATGATCAAAAGACAAGCCAGTATTATCTG
CACCTTTTTGATAAAAAACAGCCTGATTTGAATTGGGAAAATGAAAAGGTGAGGAATGCCGTTTATGACATGATCAATTG
GTGGCTTGATAAAGGAATTGACGGATTTCGGGTGGATGCCATTACGCATATTAAGAAAAAAGAAGGGTTTCCTGACATGC
CGAATCCAAAGGGACTGGACTATGTCCCTTCCTTTCCTTATCATATGAATGCTGACGGCATTATGGATTTGTTAACAGAG
CTGAAGGAAAACACATTTTCCCGCTATCCGATTATGACAGTTGGTGAAGCAAACGGCGTCGCTGCAAAAGAAGCAGCTGA
TTGGGCTGGAGAAAAAAACGGCATATTCAGCATGATTTTTCAATTTGAGCATCTCGGCCTGTGGGATGTAGAGATCAATG
AAAGTATTGATATCGTTGCATTTAAACGAATTTTAACTGACTGGCAGGACAGTCTGGAAGGGATCGGCTGGAATGCTTTG
TTTATGGAGAATCACGACCAGCCCCGTTCAGTTTCTGTGTGGGGAGACGACGGAGTGTACCTGAAAGAAAGTGCAAAAGC
GCTTTCCGCTGTTTATTTTCTCATGAAAGGCACACCGTTTATTTATCAGGGACAGGAACTCGGGATGACGAACGTGGCTT
TTCCATCTATTGAGGACTATGATGATGTCGCATTGAAACGCCTGTATGAAACAAAAACAGCGAAAGGCACATCACACGAG
GATGTGATGAAGATTGTCTGGAAAAAGGGAAGAGACAATTCGCGAACGCCGATGCAATGGAATGCTGGTCCATACGCCGG
ATTTTCTGAGGCCAAACCGTGGATCGGAATCAATGAGAATTACAAATGGCTGAATGCCGAGGCGCAAAAAAACGATAAAA
CATCCGTTTACCATTTTTACAAAAGCTTGATCAAGCTGCGCCAAACATATGATGTTTTTATTAACGGGACATACGAGCTC
ATTTTACCGGAAGATCAGCAAATCTTTGCATATCTTCGGAAAAACGAGAGCCATACTGCACTGATTGCGGCCAATCTGAC
AGGAACACCGGCGTTGTTCAGGCACTCTGGATTGCCATTGTCATCAGATGCACTCGTCTTGTCAAATATTGAAACAGAGC
CGCACAAGCATATGACATCAGTACTCTTAAAGCCGTATGAAGCAAGAATATATCTATGGTGCTAA

Upstream 100 bases:

>100_bases
TGTTTTTTGTGTCATCAAGTCATTTCTTAAAAAGCAATGCTGTAAAGACCTTTTTCTACATAGGATATATAAGAACGGTA
CACTGGGAAGGAGTGGCTGT

Downstream 100 bases:

>100_bases
ACTCCAGCCTTAGACTGGAGTTTTTTTGTTTTATCGGGTTTATGAGAGCGGTTTAACAGGAAAAAAAAGAACACAGAACG
AATTAAGCGATGAGAGCGGA

Product: oligo-1,6-glucosidase

Products: NA

Alternate protein names: Oligosaccharide alpha-1,6-glucosidase 3; Sucrase-isomaltase 3; Isomaltase 3

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKKAWWKEAVVYQIYPRSFKDSNGDGIGDIQGIRTKLSYIKELGADVIWICPLYDSPNADNGYDIRDYQNILSEFGTMED
FDELLGDIHDLDMKLIMDLVVNHTSDEHPWFIESRSSIHSEKRDWYIWKDGKNGKTPNNWESIFGGPAWEYDQKTSQYYL
HLFDKKQPDLNWENEKVRNAVYDMINWWLDKGIDGFRVDAITHIKKKEGFPDMPNPKGLDYVPSFPYHMNADGIMDLLTE
LKENTFSRYPIMTVGEANGVAAKEAADWAGEKNGIFSMIFQFEHLGLWDVEINESIDIVAFKRILTDWQDSLEGIGWNAL
FMENHDQPRSVSVWGDDGVYLKESAKALSAVYFLMKGTPFIYQGQELGMTNVAFPSIEDYDDVALKRLYETKTAKGTSHE
DVMKIVWKKGRDNSRTPMQWNAGPYAGFSEAKPWIGINENYKWLNAEAQKNDKTSVYHFYKSLIKLRQTYDVFINGTYEL
ILPEDQQIFAYLRKNESHTALIAANLTGTPALFRHSGLPLSSDALVLSNIETEPHKHMTSVLLKPYEARIYLWC

Sequences:

>Translated_554_residues
MKKAWWKEAVVYQIYPRSFKDSNGDGIGDIQGIRTKLSYIKELGADVIWICPLYDSPNADNGYDIRDYQNILSEFGTMED
FDELLGDIHDLDMKLIMDLVVNHTSDEHPWFIESRSSIHSEKRDWYIWKDGKNGKTPNNWESIFGGPAWEYDQKTSQYYL
HLFDKKQPDLNWENEKVRNAVYDMINWWLDKGIDGFRVDAITHIKKKEGFPDMPNPKGLDYVPSFPYHMNADGIMDLLTE
LKENTFSRYPIMTVGEANGVAAKEAADWAGEKNGIFSMIFQFEHLGLWDVEINESIDIVAFKRILTDWQDSLEGIGWNAL
FMENHDQPRSVSVWGDDGVYLKESAKALSAVYFLMKGTPFIYQGQELGMTNVAFPSIEDYDDVALKRLYETKTAKGTSHE
DVMKIVWKKGRDNSRTPMQWNAGPYAGFSEAKPWIGINENYKWLNAEAQKNDKTSVYHFYKSLIKLRQTYDVFINGTYEL
ILPEDQQIFAYLRKNESHTALIAANLTGTPALFRHSGLPLSSDALVLSNIETEPHKHMTSVLLKPYEARIYLWC
>Mature_554_residues
MKKAWWKEAVVYQIYPRSFKDSNGDGIGDIQGIRTKLSYIKELGADVIWICPLYDSPNADNGYDIRDYQNILSEFGTMED
FDELLGDIHDLDMKLIMDLVVNHTSDEHPWFIESRSSIHSEKRDWYIWKDGKNGKTPNNWESIFGGPAWEYDQKTSQYYL
HLFDKKQPDLNWENEKVRNAVYDMINWWLDKGIDGFRVDAITHIKKKEGFPDMPNPKGLDYVPSFPYHMNADGIMDLLTE
LKENTFSRYPIMTVGEANGVAAKEAADWAGEKNGIFSMIFQFEHLGLWDVEINESIDIVAFKRILTDWQDSLEGIGWNAL
FMENHDQPRSVSVWGDDGVYLKESAKALSAVYFLMKGTPFIYQGQELGMTNVAFPSIEDYDDVALKRLYETKTAKGTSHE
DVMKIVWKKGRDNSRTPMQWNAGPYAGFSEAKPWIGINENYKWLNAEAQKNDKTSVYHFYKSLIKLRQTYDVFINGTYEL
ILPEDQQIFAYLRKNESHTALIAANLTGTPALFRHSGLPLSSDALVLSNIETEPHKHMTSVLLKPYEARIYLWC

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI187423904, Length=536, Percent_Identity=30.410447761194, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI1790687, Length=553, Percent_Identity=39.2405063291139, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786604, Length=416, Percent_Identity=22.8365384615385, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32565753, Length=205, Percent_Identity=31.7073170731707, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25147709, Length=507, Percent_Identity=24.2603550295858, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321726, Length=581, Percent_Identity=39.5869191049914, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324416, Length=581, Percent_Identity=39.5869191049914, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322245, Length=578, Percent_Identity=38.2352941176471, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322241, Length=581, Percent_Identity=39.2426850258176, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6322021, Length=581, Percent_Identity=39.2426850258176, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321731, Length=587, Percent_Identity=39.8637137989778, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319776, Length=587, Percent_Identity=39.8637137989778, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583745, Length=532, Percent_Identity=36.4661654135338, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24583747, Length=519, Percent_Identity=35.2601156069364, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24583749, Length=519, Percent_Identity=35.2601156069364, Blast_Score=282, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24586593, Length=538, Percent_Identity=33.6431226765799, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI221330053, Length=588, Percent_Identity=32.1428571428571, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24586589, Length=489, Percent_Identity=36.1963190184049, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24586597, Length=504, Percent_Identity=33.7301587301587, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24586587, Length=591, Percent_Identity=31.6412859560068, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24586599, Length=547, Percent_Identity=31.8098720292505, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24586591, Length=500, Percent_Identity=33.8, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI45549022, Length=535, Percent_Identity=31.214953271028, Blast_Score=232, Evalue=6e-61,
Organism=Drosophila melanogaster, GI281360393, Length=484, Percent_Identity=30.7851239669421, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): O16G3_BACSU (O05242)

Other databases:

- EMBL:   Z93935
- EMBL:   AL009126
- PIR:   H70011
- RefSeq:   NP_391007.2
- HSSP:   P21332
- ProteinModelPortal:   O05242
- SMR:   O05242
- EnsemblBacteria:   EBBACT00000000103
- GeneID:   938857
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31290
- NMPDR:   fig|224308.1.peg.3132
- GenoList:   BSU31290
- GeneTree:   EBGT00070000031845
- HOGENOM:   HBG669028
- PhylomeDB:   O05242
- ProtClustDB:   CLSK887787
- BioCyc:   BSUB:BSU31290-MONOMER
- GO:   GO:0005737
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- SMART:   SM00642

Pfam domain/function: PF00128 Alpha-amylase; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.10

Molecular weight: Translated: 63970; Mature: 63970

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: ACT_SITE 199-199 ACT_SITE 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAWWKEAVVYQIYPRSFKDSNGDGIGDIQGIRTKLSYIKELGADVIWICPLYDSPNAD
CCCHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
NGYDIRDYQNILSEFGTMEDFDELLGDIHDLDMKLIMDLVVNHTSDEHPWFIESRSSIHS
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCC
EKRDWYIWKDGKNGKTPNNWESIFGGPAWEYDQKTSQYYLHLFDKKQPDLNWENEKVRNA
CCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
VYDMINWWLDKGIDGFRVDAITHIKKKEGFPDMPNPKGLDYVPSFPYHMNADGIMDLLTE
HHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LKENTFSRYPIMTVGEANGVAAKEAADWAGEKNGIFSMIFQFEHLGLWDVEINESIDIVA
HHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHH
FKRILTDWQDSLEGIGWNALFMENHDQPRSVSVWGDDGVYLKESAKALSAVYFLMKGTPF
HHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCCCE
IYQGQELGMTNVAFPSIEDYDDVALKRLYETKTAKGTSHEDVMKIVWKKGRDNSRTPMQW
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEE
NAGPYAGFSEAKPWIGINENYKWLNAEAQKNDKTSVYHFYKSLIKLRQTYDVFINGTYEL
CCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEECCEEEE
ILPEDQQIFAYLRKNESHTALIAANLTGTPALFRHSGLPLSSDALVLSNIETEPHKHMTS
EECCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCHHHHHHH
VLLKPYEARIYLWC
HHCCCCCCEEEEEC
>Mature Secondary Structure
MKKAWWKEAVVYQIYPRSFKDSNGDGIGDIQGIRTKLSYIKELGADVIWICPLYDSPNAD
CCCHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
NGYDIRDYQNILSEFGTMEDFDELLGDIHDLDMKLIMDLVVNHTSDEHPWFIESRSSIHS
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCC
EKRDWYIWKDGKNGKTPNNWESIFGGPAWEYDQKTSQYYLHLFDKKQPDLNWENEKVRNA
CCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
VYDMINWWLDKGIDGFRVDAITHIKKKEGFPDMPNPKGLDYVPSFPYHMNADGIMDLLTE
HHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LKENTFSRYPIMTVGEANGVAAKEAADWAGEKNGIFSMIFQFEHLGLWDVEINESIDIVA
HHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHH
FKRILTDWQDSLEGIGWNALFMENHDQPRSVSVWGDDGVYLKESAKALSAVYFLMKGTPF
HHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCCCE
IYQGQELGMTNVAFPSIEDYDDVALKRLYETKTAKGTSHEDVMKIVWKKGRDNSRTPMQW
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEE
NAGPYAGFSEAKPWIGINENYKWLNAEAQKNDKTSVYHFYKSLIKLRQTYDVFINGTYEL
CCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEECCEEEE
ILPEDQQIFAYLRKNESHTALIAANLTGTPALFRHSGLPLSSDALVLSNIETEPHKHMTS
EECCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCHHHHHHH
VLLKPYEARIYLWC
HHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377