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The map label for this gene is ytwF

Identifier: 255767695

GI number: 255767695

Start: 3102201

End: 3102512

Strand: Reverse

Name: ytwF

Synonym: BSU30310

Alternate gene names: 255767695

Gene position: 3102512-3102201 (Counterclockwise)

Preceding gene: 16080084

Following gene: 16080077

Centisome position: 73.6

GC content: 42.95

Gene sequence:

>312_bases
TTGGAAATAAAAGAAATCAGCACAGCCGCTTTGAAAGAAAAAATTGAGGCGGACGAAGAATTATATTTAATTGATGTCAG
AGAAGATGAAGAGGTAGCGGAAGGCATGATCCCGCAAGCCGTTCATATTCGCATGGGGGATATTCCTGAAAAAATGGAAA
CCCTTGATAAAGACAAAGAGTACGTATTTATCTGCCGCTCAGGAATGCGCAGCATGAACGTCTGCAAGTACCTGGATGAG
CAAGGCTTTAAAACAGTCAATGTTGAAGGCGGCATGATGGCCTGGGAAGGCGAAACAAAACCAAAAAACTAG

Upstream 100 bases:

>100_bases
CTTTGCCAAAAGGGGATTTTTTTTCATCAGTCTCAGAATAGCAATCGTTCGGCTGACTTTGATATACTGAACATGATCAC
TGTGAATAGGAGTGTTTTCT

Downstream 100 bases:

>100_bases
GGGAGAGCGGTCCCCTAGTTTTTATTTGTATTCCGGAAGCCAATCGCCGTGTGCTGCAAATAAATCGTCGCACATGGAGA
TGATGTCCTTCATGGAAAGC

Product: sulfur transferase

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MEIKEISTAALKEKIEADEELYLIDVREDEEVAEGMIPQAVHIRMGDIPEKMETLDKDKEYVFICRSGMRSMNVCKYLDE
QGFKTVNVEGGMMAWEGETKPKN

Sequences:

>Translated_103_residues
MEIKEISTAALKEKIEADEELYLIDVREDEEVAEGMIPQAVHIRMGDIPEKMETLDKDKEYVFICRSGMRSMNVCKYLDE
QGFKTVNVEGGMMAWEGETKPKN
>Mature_103_residues
MEIKEISTAALKEKIEADEELYLIDVREDEEVAEGMIPQAVHIRMGDIPEKMETLDKDKEYVFICRSGMRSMNVCKYLDE
QGFKTVNVEGGMMAWEGETKPKN

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YTWF_BACSU (O32072)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_390909.2
- ProteinModelPortal:   O32072
- SMR:   O32072
- EnsemblBacteria:   EBBACT00000001920
- GeneID:   936589
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30310
- GenoList:   BSU30310
- GeneTree:   EBGT00050000000398
- HOGENOM:   HBG743107
- ProtClustDB:   CLSK2539633
- BioCyc:   BSUB:BSU30310-MONOMER
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11835; Mature: 11835

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
7.8 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
7.8 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKEISTAALKEKIEADEELYLIDVREDEEVAEGMIPQAVHIRMGDIPEKMETLDKDKE
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHHCCCCC
YVFICRSGMRSMNVCKYLDEQGFKTVNVEGGMMAWEGETKPKN
EEEEECCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure
MEIKEISTAALKEKIEADEELYLIDVREDEEVAEGMIPQAVHIRMGDIPEKMETLDKDKE
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHHCCCCC
YVFICRSGMRSMNVCKYLDEQGFKTVNVEGGMMAWEGETKPKN
EEEEECCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377