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The map label for this gene is leuS

Identifier: 16080084

GI number: 16080084

Start: 3102629

End: 3105043

Strand: Reverse

Name: leuS

Synonym: BSU30320

Alternate gene names: 16080084

Gene position: 3105043-3102629 (Counterclockwise)

Preceding gene: 255767696

Following gene: 255767695

Centisome position: 73.66

GC content: 47.74

Gene sequence:

>2415_bases
TTGAGTTTTCAGCACAAAGAGATAGAAAAGAAATGGCAGACATATTGGCTTGAAAACAAAACATTTGCCACTCTTGATAA
TAATGAAAAACAAAAATTTTACGCGCTGGACATGTTTCCTTATCCGTCTGGAGCTGGGTTGCACGTCGGCCATCCTGAAG
GATACACAGCTACGGATATTCTGTCCCGCATGAAGCGCATGCAGGGCTATGATGTCCTTCATCCAATGGGCTGGGACGCA
TTCGGCCTGCCAGCTGAACAGTACGCGCTTGACACAGGGAACGACCCCGCTGTGTTTACGAAGCAGAATATTGATAACTT
CCGCCGCCAAATTCAAGCGCTTGGCTTCTCATATGACTGGGATCGCGAAATCAATACGACTGACCCTGAATACTATAAAT
GGACGCAATGGATTTTCTTAAAGCTATACGAAAAAGGCCTTGCTTACGTTGACGAAGTGCCTGTAAACTGGTGCCCTGCG
CTCGGTACTGTTCTTGCCAACGAAGAAGTCATTGACGGCAAGAGCGAACGCGGCGGCCATCCGGTAGAGAGACGCCCAAT
GAAGCAGTGGATGCTGAAAATCACCGCTTATGCGGACAGGCTCCTTGAGGACTTGGAAGAGCTTGATTGGCCGGAAAGCA
TTAAAGATATGCAGCGCAACTGGATCGGCCGTTCGGAAGGCGCTCACGTTCATTTTGCTATAGATGGACATGATGATTCC
TTTACAGTGTTTACAACAAGACCAGATACGCTGTTTGGCGCTACATACACTGTCCTTGCCCCGGAACACGCATTGGTGGA
AAACATCACAACGGCAGAGCAAAAAGAAGCTGTTGAAGCTTATATCAAAGAAATTCAATCAAAGAGTGACCTAGAACGCA
CAGATCTTGCGAAAACAAAGACAGGCGTATTTACAGGAGCGTATGCGATCAATCCTGTAAACGGAGAAAAACTGCCGATT
TGGATTGCGGATTATGTTCTTGCATCATACGGAACAGGTGCTGTCATGGCAGTTCCAGGACACGATGAGCGTGATTTTGA
ATTCGCCAAAACATTCGGCCTTCCGGTGAAGGAAGTCGTAAAAGGCGGGAACGTTGAGGAAGCAGCCTATACTGGCGACG
GCGAGCACGTGAACTCTGATTTCCTGAACGGCCTTCACAAACAGGAAGCGATTGAAAAAGTGATCGCTTGGCTGGAAGAA
ACGAAAAACGGTGAGAAGAAAGTGACGTACCGTCTTCGTGACTGGCTCTTCAGCCGCCAGCGTTATTGGGGCGAGCCGAT
TCCGGTCATTCATTGGGAAGACGGAACGTCAACAGCTGTCCCGGAAGAGGAGCTGCCGCTGATTTTGCCAAAAACGGATG
AAATCAAACCGAGCGGAACGGGCGAATCACCGCTTGCGAACATTAAAGAGTGGGTGGAAGTCACAGACCCTGAGACAGGG
AAAAAAGGAAGAAGAGAAACGAATACAATGCCGCAATGGGCGGGAAGCTGCTGGTATTTCTTGCGCTATATTGATCCGCA
CAATCCGGATCAGCTGGCATCACCAGAAAAATTGGAAAAATGGCTTCCGGTCGATATGTATATCGGCGGTGCAGAACATG
CCGTGCTTCACCTTCTGTATGCCCGCTTCTGGCATAAGTTCCTTTATGATATCGGCGTAGTGCCGACGAAAGAACCGTTC
CAAAAGCTGTACAACCAAGGAATGATTCTCGGCGAAAACAACGAAAAAATGAGTAAATCTAAAGGGAACGTTGTCAATCC
TGACGAAATCGTGGCCTCTCACGGTGCTGATACGCTGAGATTGTACGAAATGTTCATGGGACCTCTTGATGCTTCAATCG
CCTGGTCTGAATCAGGATTAGACGGTGCGCGCCGTTTCCTTGACCGTGTATGGCGCCTATTTATTGAAGACAGCGGTGAG
CTTAATGGAAAAATCGTTGAAGGCGCGGGTGAAACATTGGAGCGCGTCTATCATGAAACGGTCATGAAAGTCACAGACCA
TTATGAAGGCCTTCGTTTCAACACGGGTATTTCCCAGCTGATGGTCTTTATTAATGAAGCTTATAAAGCAACAGAACTGC
CGAAAGAATATATGGAAGGCTTCGTGAAGCTTCTTTCTCCTGTCGCGCCACACTTAGCGGAAGAGCTATGGGAGAAGCTT
GGCCATTCCGGCACAATTGCCTACGAAGCTTGGCCTGTATATGATGAAACAAAACTTGTGGATGATGAAGTTGAAATCGT
TGTTCAGCTGAACGGAAAAGTAAAAGCGAAATTACAGGTTCCTGCCGATGCAACGAAAGAACAGCTGGAACAGCTTGCTC
AAGCAGATGAAAAGGTCAAAGAGCAGCTTGAAGGCAAAACGATTCGGAAAATCATCGCGGTGCCTGGGAAGCTTGTCAAT
ATTGTGGCAAACTAA

Upstream 100 bases:

>100_bases
GGAGTTTTTTTTAGTTTGGGAATATTCTGAATATAATCCTCTCCTTCCCGTCAATACTTGAACCATACACGCCGTATTCA
ATCAGAAGGAGGTTTTTACT

Downstream 100 bases:

>100_bases
GCCTAGAAAAAATCCCCTTTGCCAAAAGGGGATTTTTTTTCATCAGTCTCAGAATAGCAATCGTTCGGCTGACTTTGATA
TACTGAACATGATCACTGTG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSFQHKEIEKKWQTYWLENKTFATLDNNEKQKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYDVLHPMGWDA
FGLPAEQYALDTGNDPAVFTKQNIDNFRRQIQALGFSYDWDREINTTDPEYYKWTQWIFLKLYEKGLAYVDEVPVNWCPA
LGTVLANEEVIDGKSERGGHPVERRPMKQWMLKITAYADRLLEDLEELDWPESIKDMQRNWIGRSEGAHVHFAIDGHDDS
FTVFTTRPDTLFGATYTVLAPEHALVENITTAEQKEAVEAYIKEIQSKSDLERTDLAKTKTGVFTGAYAINPVNGEKLPI
WIADYVLASYGTGAVMAVPGHDERDFEFAKTFGLPVKEVVKGGNVEEAAYTGDGEHVNSDFLNGLHKQEAIEKVIAWLEE
TKNGEKKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTSTAVPEEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
KKGRRETNTMPQWAGSCWYFLRYIDPHNPDQLASPEKLEKWLPVDMYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPF
QKLYNQGMILGENNEKMSKSKGNVVNPDEIVASHGADTLRLYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEDSGE
LNGKIVEGAGETLERVYHETVMKVTDHYEGLRFNTGISQLMVFINEAYKATELPKEYMEGFVKLLSPVAPHLAEELWEKL
GHSGTIAYEAWPVYDETKLVDDEVEIVVQLNGKVKAKLQVPADATKEQLEQLAQADEKVKEQLEGKTIRKIIAVPGKLVN
IVAN

Sequences:

>Translated_804_residues
MSFQHKEIEKKWQTYWLENKTFATLDNNEKQKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYDVLHPMGWDA
FGLPAEQYALDTGNDPAVFTKQNIDNFRRQIQALGFSYDWDREINTTDPEYYKWTQWIFLKLYEKGLAYVDEVPVNWCPA
LGTVLANEEVIDGKSERGGHPVERRPMKQWMLKITAYADRLLEDLEELDWPESIKDMQRNWIGRSEGAHVHFAIDGHDDS
FTVFTTRPDTLFGATYTVLAPEHALVENITTAEQKEAVEAYIKEIQSKSDLERTDLAKTKTGVFTGAYAINPVNGEKLPI
WIADYVLASYGTGAVMAVPGHDERDFEFAKTFGLPVKEVVKGGNVEEAAYTGDGEHVNSDFLNGLHKQEAIEKVIAWLEE
TKNGEKKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTSTAVPEEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
KKGRRETNTMPQWAGSCWYFLRYIDPHNPDQLASPEKLEKWLPVDMYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPF
QKLYNQGMILGENNEKMSKSKGNVVNPDEIVASHGADTLRLYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEDSGE
LNGKIVEGAGETLERVYHETVMKVTDHYEGLRFNTGISQLMVFINEAYKATELPKEYMEGFVKLLSPVAPHLAEELWEKL
GHSGTIAYEAWPVYDETKLVDDEVEIVVQLNGKVKAKLQVPADATKEQLEQLAQADEKVKEQLEGKTIRKIIAVPGKLVN
IVAN
>Mature_803_residues
SFQHKEIEKKWQTYWLENKTFATLDNNEKQKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYDVLHPMGWDAF
GLPAEQYALDTGNDPAVFTKQNIDNFRRQIQALGFSYDWDREINTTDPEYYKWTQWIFLKLYEKGLAYVDEVPVNWCPAL
GTVLANEEVIDGKSERGGHPVERRPMKQWMLKITAYADRLLEDLEELDWPESIKDMQRNWIGRSEGAHVHFAIDGHDDSF
TVFTTRPDTLFGATYTVLAPEHALVENITTAEQKEAVEAYIKEIQSKSDLERTDLAKTKTGVFTGAYAINPVNGEKLPIW
IADYVLASYGTGAVMAVPGHDERDFEFAKTFGLPVKEVVKGGNVEEAAYTGDGEHVNSDFLNGLHKQEAIEKVIAWLEET
KNGEKKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTSTAVPEEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETGK
KGRRETNTMPQWAGSCWYFLRYIDPHNPDQLASPEKLEKWLPVDMYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQ
KLYNQGMILGENNEKMSKSKGNVVNPDEIVASHGADTLRLYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEDSGEL
NGKIVEGAGETLERVYHETVMKVTDHYEGLRFNTGISQLMVFINEAYKATELPKEYMEGFVKLLSPVAPHLAEELWEKLG
HSGTIAYEAWPVYDETKLVDDEVEIVVQLNGKVKAKLQVPADATKEQLEQLAQADEKVKEQLEGKTIRKIIAVPGKLVNI
VAN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=872, Percent_Identity=33.8302752293578, Blast_Score=477, Evalue=1e-134,
Organism=Homo sapiens, GI94721239, Length=181, Percent_Identity=28.7292817679558, Blast_Score=67, Evalue=8e-11,
Organism=Homo sapiens, GI94721241, Length=181, Percent_Identity=28.7292817679558, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI1786861, Length=863, Percent_Identity=43.3371958285052, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=398, Percent_Identity=24.8743718592965, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71997510, Length=816, Percent_Identity=30.1470588235294, Blast_Score=334, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71997517, Length=810, Percent_Identity=30.2469135802469, Blast_Score=333, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI212645227, Length=373, Percent_Identity=28.686327077748, Blast_Score=136, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17554638, Length=277, Percent_Identity=25.6317689530686, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323414, Length=869, Percent_Identity=36.5937859608746, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6321531, Length=379, Percent_Identity=24.2744063324538, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355409, Length=876, Percent_Identity=30.9360730593607, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281366294, Length=374, Percent_Identity=25.668449197861, Blast_Score=78, Evalue=3e-14,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91543; Mature: 91412

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQHKEIEKKWQTYWLENKTFATLDNNEKQKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHEECCCEEEECCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMKRMQGYDVLHPMGWDAFGLPAEQYALDTGNDPAVFTKQNIDNFRRQIQALGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCC
DREINTTDPEYYKWTQWIFLKLYEKGLAYVDEVPVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMKQWMLKITAYADRLLEDLEELDWPESIKDMQRNWIGRSEGAHVHFAIDGHDDS
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCC
FTVFTTRPDTLFGATYTVLAPEHALVENITTAEQKEAVEAYIKEIQSKSDLERTDLAKTK
EEEEECCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TGVFTGAYAINPVNGEKLPIWIADYVLASYGTGAVMAVPGHDERDFEFAKTFGLPVKEVV
CCEEECCEEECCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCHHHHH
KGGNVEEAAYTGDGEHVNSDFLNGLHKQEAIEKVIAWLEETKNGEKKVTYRLRDWLFSRQ
CCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RYWGEPIPVIHWEDGTSTAVPEEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
HHCCCCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
KKGRRETNTMPQWAGSCWYFLRYIDPHNPDQLASPEKLEKWLPVDMYIGGAEHAVLHLLY
CCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHH
ARFWHKFLYDIGVVPTKEPFQKLYNQGMILGENNEKMSKSKGNVVNPDEIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCCCHHHHCCCCCCCHHHHHHHCCCHHHH
LYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEDSGELNGKIVEGAGETLERVYHET
HHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHH
VMKVTDHYEGLRFNTGISQLMVFINEAYKATELPKEYMEGFVKLLSPVAPHLAEELWEKL
HHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GHSGTIAYEAWPVYDETKLVDDEVEIVVQLNGKVKAKLQVPADATKEQLEQLAQADEKVK
CCCCEEEEEEECCCCCCEECCCCEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
EQLEGKTIRKIIAVPGKLVNIVAN
HHHCCHHHHHHHHCCCHHHHEECC
>Mature Secondary Structure 
SFQHKEIEKKWQTYWLENKTFATLDNNEKQKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCHHHHHHHHHHHEECCCEEEECCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMKRMQGYDVLHPMGWDAFGLPAEQYALDTGNDPAVFTKQNIDNFRRQIQALGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCC
DREINTTDPEYYKWTQWIFLKLYEKGLAYVDEVPVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMKQWMLKITAYADRLLEDLEELDWPESIKDMQRNWIGRSEGAHVHFAIDGHDDS
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCC
FTVFTTRPDTLFGATYTVLAPEHALVENITTAEQKEAVEAYIKEIQSKSDLERTDLAKTK
EEEEECCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TGVFTGAYAINPVNGEKLPIWIADYVLASYGTGAVMAVPGHDERDFEFAKTFGLPVKEVV
CCEEECCEEECCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCHHHHH
KGGNVEEAAYTGDGEHVNSDFLNGLHKQEAIEKVIAWLEETKNGEKKVTYRLRDWLFSRQ
CCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RYWGEPIPVIHWEDGTSTAVPEEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
HHCCCCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
KKGRRETNTMPQWAGSCWYFLRYIDPHNPDQLASPEKLEKWLPVDMYIGGAEHAVLHLLY
CCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHH
ARFWHKFLYDIGVVPTKEPFQKLYNQGMILGENNEKMSKSKGNVVNPDEIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCCCHHHHCCCCCCCHHHHHHHCCCHHHH
LYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEDSGELNGKIVEGAGETLERVYHET
HHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHH
VMKVTDHYEGLRFNTGISQLMVFINEAYKATELPKEYMEGFVKLLSPVAPHLAEELWEKL
HHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GHSGTIAYEAWPVYDETKLVDDEVEIVVQLNGKVKAKLQVPADATKEQLEQLAQADEKVK
CCCCEEEEEEECCCCCCEECCCCEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
EQLEGKTIRKIIAVPGKLVNIVAN
HHHCCHHHHHHHHCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA