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The map label for this gene is amyX

Identifier: 255767686

GI number: 255767686

Start: 3061651

End: 3063807

Strand: Reverse

Name: amyX

Synonym: BSU29930

Alternate gene names: 255767686

Gene position: 3063807-3061651 (Counterclockwise)

Preceding gene: 16080046

Following gene: 255767685

Centisome position: 72.68

GC content: 47.71

Gene sequence:

>2157_bases
ATGGTCAGCATCCGCCGCAGCTTCGAAGCGTATGTCGATGACATGAATATCATTACTGTTCTGATTCCTGCTGAACAAAA
GGAAATCATGACACCGCCGTTTCGGCTTGAGACAGAAATAACAGATTTTCCTCTGGCTGTCAGGGAGGAATACTCCCTTG
AAGCAAAATACAAGTACGTCTGCGTATCCGACCATCCTGTGACATTTGGAAAAATCCATTGCGTCAGAGCATCCAGCGGC
CACAAAACGGATCTCCAAATTGGCGCGGTCATCCGGACGGCAGCGTTTGATGACGAATTTTATTATGACGGAGAGCTGGG
CGCCGTTTATACCGCGGATCATACCGTATTTAAAGTATGGGCGCCTGCTGCAACCTCAGCTGCTGTCAAGCTTTCACACC
CCAATAAAAGCGGGCGCACATTCCAAATGACTCGCTTGGAAAAAGGCGTCTATGCCGTTACGGTCACAGGTGACCTTCAC
GGATATGAGTATTTGTTTTGCATCTGCAACAATTCAGAATGGATGGAAACAGTTGACCAGTATGCCAAGGCTGTGACTGT
AAATGGAGAGAAGGGCGTCGTCTTGCGCCCGGATCAAATGAAATGGACTGCTCCTCTTAAACCATTCTCACACCCTGTGG
ATGCCGTCATCTATGAGACGCATCTTCGCGACTTCTCCATCCATGAAAACAGCGGCATGATAAACAAGGGAAAATACTTA
GCGCTGACGGAAACTGATACACAAACCGCAAATGGCAGTTCTTCGGGATTAGCGTATGTAAAAGAGCTTGGTGTGACACA
TGTGGAGCTTCTGCCGGTGAATGATTTTGCCGGAGTTGATGAAGAGAAGCCGCTTGATGCATACAATTGGGGATATAACC
CGCTTCATTTCTTTGCCCCGGAGGGAAGCTATGCCTCAAATCCTCATGATCCTCAAACGAGAAAAACAGAGCTGAAACAA
ATGATCAATACCCTGCATCAGCACGGTCTGCGAGTCATTCTGGATGTTGTTTTTAACCATGTGTATAAGAGGGAGAATTC
CCCCTTTGAAAAGACAGTGCCCGGTTATTTTTTCCGGCACGACGAATGTGGGATGCCATCAAACGGCACCGGCGTTGGCA
ATGATATTGCATCAGAAAGAAGGATGGCAAGAAAATTCATTGCGGATTGCGTGGTCTATTGGCTTGAAGAATACAATGTT
GACGGCTTCCGCTTTGATCTCCTCGGGATTTTAGATATTGACACCGTGCTTTATATGAAAGAGAAAGCAACTAAGGCAAA
GCCCGGAATCCTGCTTTTTGGAGAAGGGTGGGACCTGGCTACACCGCTGCCGCATGAACAGAAAGCTGCTTTGGCGAACG
CGCCAAGAATGCCGGGCATCGGCTTTTTTAATGATATGTTTCGTGACGCTGTAAAAGGGAACACCTTTCACCTTAAGGCA
ACAGGGTTTGCGCTCGGCAACGGTGAATCAGCACAAGCTGTGATGCATGGAATTGCCGGGTCTTCCGGATGGAAGGCATT
AGCACCGATTGTTCCGGAACCAAGCCAGTCCATCAATTATGTCGAATCACACGACAATCACACCTTTTGGGATAAAATGA
GCTTTGCGCTTCCTCAAGAAAATGACAGCCGAAAGCGAAGCAGGCAAAGGCTTGCAGCCGCGATTATTTTGCTTGCCCAA
GGGGTGCCGTTTATTCACAGCGGCCAGGAATTTTTCCGGACGAAGCAGGGAGTGGAAAACAGCTATCAATCCAGTGACAG
CATCAACCAGCTCGACTGGGATCGCCGTGAAACATTCAAAGAAGATGTTCACTATATCCGCAGGCTGATCTCGCTGAGAA
AAGCGCATCCTGCATTCCGTCTTAGGTCCGCTGCAGACATCCAGCGCCATCTTGAATGCTTGACGCTAAAAGAACACCTT
ATCGCATACAGGCTTTATGATCTTGACGAGGTTGACGAATGGAAAGATATCATTGTTATCCATCACGCGAGTCCAGACTC
CGTCGAGTGGAGGCTGCCAAACGACATACCTTATCGGCTTTTATGTGATCCATCAGGATTTCAGGAAGACCCAACAGAAA
TCAAGAAAACGGTTGCAGTAAACGGCATCGGAACGGTTATCTTATATTTAGCATCAGATCTTAAGAGTTTTGCTTGA

Upstream 100 bases:

>100_bases
GCTGACGGAGAAATCATGGGCACTACCCCGTTTCGTCTGGCATCTAGTCCTTCACCCCTAAGAATCAAAACATAAACGTG
TTTGGAGGAGAGAGCTTCCG

Downstream 100 bases:

>100_bases
CGAAGTTTGTGCTATAGCGCTAAAATTTAAAGGACGGCTATTGATTAGCATATGAGACTCTCAATAGCTGTCTTTTTTAT
TTTTATACGATTCGTACGAA

Product: pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSG
HKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLH
GYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYL
ALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQ
MINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNV
DGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKA
TGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAAAIILLAQ
GVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHL
IAYRLYDLDEVDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA

Sequences:

>Translated_718_residues
MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSG
HKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLH
GYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYL
ALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQ
MINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNV
DGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKA
TGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAAAIILLAQ
GVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHL
IAYRLYDLDEVDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA
>Mature_718_residues
MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSG
HKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLH
GYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYL
ALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQ
MINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNV
DGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKA
TGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAAAIILLAQ
GVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHL
IAYRLYDLDEVDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI2367229, Length=470, Percent_Identity=27.4468085106383, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1789839, Length=308, Percent_Identity=25.6493506493506, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PULA_BACSU (C0SPA0)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69585
- RefSeq:   NP_390871.2
- PDB:   2E8Y
- PDB:   2E8Z
- PDB:   2E9B
- PDBsum:   2E8Y
- PDBsum:   2E8Z
- PDBsum:   2E9B
- ProteinModelPortal:   C0SPA0
- SMR:   C0SPA0
- EnsemblBacteria:   EBBACT00000000438
- GeneID:   937292
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29930
- NMPDR:   fig|224308.1.peg.2996
- GenoList:   BSU29930
- GeneTree:   EBGT00070000031950
- HOGENOM:   HBG697609
- ProtClustDB:   CLSK873560
- BioCyc:   BSUB:BSU29930-MONOMER
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR02104

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.41

Molecular weight: Translated: 81078; Mature: 81078

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: ACT_SITE 406-406 ACT_SITE 435-435

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYV
CCCHHHHHHHHHCCCEEEEEEEECCCHHHCCCCCEEECCCCCCCCHHHHCCCCCEEEEEE
CVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVW
EECCCCCEEEEEEEEECCCCCCCCEEEHHEEEEEECCCCEEECCCCCEEEECCCEEEEEE
APAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLHGYEYLFCICNNSEWMETVDQ
CCCCCCEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCEEEEEEEECCCHHHHHHHH
YAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYL
HHHEEEECCCCCEEECCCCCEEECCCCCCCCCHHHEEEEECCCCEEEECCCCCEECCCEE
ALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAP
EEEECCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECC
EGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEC
DECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDIDTVLYMK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHH
EKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKA
HHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEE
TGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQE
EEEEECCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCHHHHHEECCCCC
NDSRKRSRQRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFK
CCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
EDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
HHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA
ECCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYV
CCCHHHHHHHHHCCCEEEEEEEECCCHHHCCCCCEEECCCCCCCCHHHHCCCCCEEEEEE
CVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVW
EECCCCCEEEEEEEEECCCCCCCCEEEHHEEEEEECCCCEEECCCCCEEEECCCEEEEEE
APAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLHGYEYLFCICNNSEWMETVDQ
CCCCCCEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCEEEEEEEECCCHHHHHHHH
YAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYL
HHHEEEECCCCCEEECCCCCEEECCCCCCCCCHHHEEEEECCCCEEEECCCCCEECCCEE
ALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAP
EEEECCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECC
EGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEC
DECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDIDTVLYMK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHH
EKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKA
HHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEE
TGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQE
EEEEECCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCHHHHHEECCCCC
NDSRKRSRQRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFK
CCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
EDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
HHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA
ECCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377