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The map label for this gene is mutM

Identifier: 255767671

GI number: 255767671

Start: 2972329

End: 2973159

Strand: Reverse

Name: mutM

Synonym: BSU29080

Alternate gene names: 255767671

Gene position: 2973159-2972329 (Counterclockwise)

Preceding gene: 16079961

Following gene: 255767670

Centisome position: 70.53

GC content: 47.17

Gene sequence:

>831_bases
GTGCCGGAATTACCAGAGGTTGAGACGGTCCGGCGCACTCTGACCGGGCTTGTAAAGGGAAAAACAATCAAATCGGTAGA
GATCAGATGGCCGAATATCATCAAACGGCCTGCCGAACCGGAGGAATTTGCGCGAAAACTAGCAGGAGAAACGATACAGT
CCATCGGAAGACGGGGAAAGTTTTTACTGTTTCATTTAGATCATTATGTTATGGTTTCTCACCTTCGAATGGAAGGAAAA
TACGGTCTTCATCAAGCCGAGGAGCCTGACGATAAACACGTGCATGTCATATTCACGATGACGGATGGAACCCAGCTCCG
GTACAGGGATGTGCGGAAATTTGGAACCATGCACTTATTTAAACCGGGAGAAGAAGCGGGCGAGCTCCCGCTTTCTCAGT
TAGGGCCGGAGCCGGATGCAGAAGAATTTACAAGTGCGTATTTAAAAGACCGGCTTGCGAAAACAAACCGCGCTGTCAAA
ACTGCCCTACTGGATCAAAAAACGGTAGTTGGACTCGGGAACATTTATGTGGATGAGGCTCTTTTCAGAGCGGGTGTCCA
TCCCGAGACAAAAGCAAATCAATTATCAGATAAAACAATCAAAACCCTTCACGCTGAAATCAAAAACACTCTGCAGGAGG
CGATTGATGCGGGCGGAAGCACAGTCCGTTCATATATCAACTCCCAAGGGGAAATCGGAATGTTCCAGCTGCAGCATTTT
GTGTACGGAAAAAAAGACGAGCCATGCAAAAATTGCGGAACGATGATTTCGAAAATTGTCGTTGGAGGAAGAGGCACGCA
TTTTTGCGCAAAGTGCCAGACAAAAAAGTAG

Upstream 100 bases:

>100_bases
ATGGAGCATGCGCTTGCATTAGATGTGCCGTTAAAGGTGGACTTTGCATCAGGCCCATCTTGGTACGATGCGAAATAAAC
AGAGATAGGAAGTGATGGAT

Downstream 100 bases:

>100_bases
CATAATGCTTCATTGTCCAAGCTGTCGGTGCATATATCAGTAACTCTGGATTAAGGATGCCATTTTCGAAACACGGATTT
TTCACTTTCCCATATATATG

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MPELPEVETVRRTLTGLVKGKTIKSVEIRWPNIIKRPAEPEEFARKLAGETIQSIGRRGKFLLFHLDHYVMVSHLRMEGK
YGLHQAEEPDDKHVHVIFTMTDGTQLRYRDVRKFGTMHLFKPGEEAGELPLSQLGPEPDAEEFTSAYLKDRLAKTNRAVK
TALLDQKTVVGLGNIYVDEALFRAGVHPETKANQLSDKTIKTLHAEIKNTLQEAIDAGGSTVRSYINSQGEIGMFQLQHF
VYGKKDEPCKNCGTMISKIVVGGRGTHFCAKCQTKK

Sequences:

>Translated_276_residues
MPELPEVETVRRTLTGLVKGKTIKSVEIRWPNIIKRPAEPEEFARKLAGETIQSIGRRGKFLLFHLDHYVMVSHLRMEGK
YGLHQAEEPDDKHVHVIFTMTDGTQLRYRDVRKFGTMHLFKPGEEAGELPLSQLGPEPDAEEFTSAYLKDRLAKTNRAVK
TALLDQKTVVGLGNIYVDEALFRAGVHPETKANQLSDKTIKTLHAEIKNTLQEAIDAGGSTVRSYINSQGEIGMFQLQHF
VYGKKDEPCKNCGTMISKIVVGGRGTHFCAKCQTKK
>Mature_275_residues
PELPEVETVRRTLTGLVKGKTIKSVEIRWPNIIKRPAEPEEFARKLAGETIQSIGRRGKFLLFHLDHYVMVSHLRMEGKY
GLHQAEEPDDKHVHVIFTMTDGTQLRYRDVRKFGTMHLFKPGEEAGELPLSQLGPEPDAEEFTSAYLKDRLAKTNRAVKT
ALLDQKTVVGLGNIYVDEALFRAGVHPETKANQLSDKTIKTLHAEIKNTLQEAIDAGGSTVRSYINSQGEIGMFQLQHFV
YGKKDEPCKNCGTMISKIVVGGRGTHFCAKCQTKK

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=278, Percent_Identity=39.9280575539568, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786932, Length=281, Percent_Identity=26.3345195729537, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_BACSU (O34403)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   B69663
- RefSeq:   NP_390786.2
- ProteinModelPortal:   O34403
- SMR:   O34403
- EnsemblBacteria:   EBBACT00000003807
- GeneID:   936741
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29080
- NMPDR:   fig|224308.1.peg.2911
- GenoList:   BSU29080
- GeneTree:   EBGT00050000000934
- HOGENOM:   HBG690070
- PhylomeDB:   O34403
- ProtClustDB:   PRK01103
- BioCyc:   BSUB:BSU29080-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31002; Mature: 30871

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 60-60 ACT_SITE 264-264 BINDING 93-93 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRRTLTGLVKGKTIKSVEIRWPNIIKRPAEPEEFARKLAGETIQSIGRRGK
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
FLLFHLDHYVMVSHLRMEGKYGLHQAEEPDDKHVHVIFTMTDGTQLRYRDVRKFGTMHLF
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCEEEE
KPGEEAGELPLSQLGPEPDAEEFTSAYLKDRLAKTNRAVKTALLDQKTVVGLGNIYVDEA
CCCCHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHH
LFRAGVHPETKANQLSDKTIKTLHAEIKNTLQEAIDAGGSTVRSYINSQGEIGMFQLQHF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
VYGKKDEPCKNCGTMISKIVVGGRGTHFCAKCQTKK
HCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
PELPEVETVRRTLTGLVKGKTIKSVEIRWPNIIKRPAEPEEFARKLAGETIQSIGRRGK
CCCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
FLLFHLDHYVMVSHLRMEGKYGLHQAEEPDDKHVHVIFTMTDGTQLRYRDVRKFGTMHLF
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCEEEE
KPGEEAGELPLSQLGPEPDAEEFTSAYLKDRLAKTNRAVKTALLDQKTVVGLGNIYVDEA
CCCCHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHH
LFRAGVHPETKANQLSDKTIKTLHAEIKNTLQEAIDAGGSTVRSYINSQGEIGMFQLQHF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
VYGKKDEPCKNCGTMISKIVVGGRGTHFCAKCQTKK
HCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377