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The map label for this gene is adhB

Identifier: 255767621

GI number: 255767621

Start: 2753452

End: 2754588

Strand: Direct

Name: adhB

Synonym: BSU26970

Alternate gene names: 255767621

Gene position: 2753452-2754588 (Clockwise)

Preceding gene: 16079749

Following gene: 16079751

Centisome position: 65.32

GC content: 43.18

Gene sequence:

>1137_bases
ATGAAGGCAGTAACGTATCAAGGCATTAAAAATGTTGTTGTCAAAGATGTCCCCGATCCAAAGATTGAAAAATCCGATGA
CATGATTATCAAAGTCACCAGTACAGCCATTTGCGGATCAGATTTACATTTAATCCATGGATTCATTCCGAATATGCAAG
AAGACTATGTCATCGGCCATGAACCGATGGGAATCGTCGAAGAAGTTGGTTCTGGGGTGACTAAACTAAAAAAGGGAGAT
CGGGTAATTATTCCCTTTAATATAGCATGCGGGGAATGCTTTTTTTGTAAAAACCAGCTGGAAAGCCAATGTGATCAGTC
TAATGACAATGGGGAAATGGGTGCTTATTTCGGCTATTCAGGGCAAACTGGCGGTTATCCAGGCGGGCAAGCTGAATATT
TAAGAGTGCCGTTTGCGAATTTTACCCATTTTAAAATCCCTGAATCTTGTGAGGAACCCGATGAGAAATTAAGCGTGATT
GCCGATGCCATGACCACCGGCTTTTGGAGTGTGGATAATGCCGGCGTAAAAAAAGGCGATACAGTTATCGTTCTCGGCTG
CGGACCAGTCGGCCTGTTTGCTCAAAAGTTTTGTTGGCTAAAAGGCGCAAAACGCGTCATAGCAGTTGACTATGTAAACT
ATCGCTTACAGCATGCGAAACGTACAAACAAAGTAGAAATCGTTAATTTTGAAGACCATGAGAATACAGGGAATTATTTA
AAGGAAATCACGAAAGGCGGAGCGGATGTAGTCATTGACGCTGTTGGGATGGATGGTAAAATGAGCGATCTCGAGTTCCT
TGCCAGCGGCTTAAAGCTTCATGGCGGAACGATGAGTGCATTGGTCATTGCTTCCCAAGCTGTTCGCAAAGGAGGAACCA
TACAAATTACAGGTGTGTATGGAGGCAGATATAACGGTTTTCCGTTAGGAGATATTATGCAGCGAAACGTCAATATACGC
TCTGGACAGGCTCCAGTGATCCACTATATGCCGTACATGTTTGAACTAGTATCGACAGGCAAAATTGATCCGGGAGATGT
TGTCAGCCATGTCCTGCCGCTTAGTGAAGCCAAGCATGGCTATGACATTTTTGATTCAAAAATGGATGATTGTATCAAAG
TTGTGTTAAAGCCTTAA

Upstream 100 bases:

>100_bases
GATCATGGTCGGCAATATGAACCTCTTTCCTGATGAAACAAATCGCAAAGGGATGTTTGACCGGACACCTGATGAACACT
AACACTGGAGGTTTTACAGC

Downstream 100 bases:

>100_bases
AAAACGGAGGTTACAGAAATGGAATACGCCTTACACGAAGTGCTTGAGGTTCAGGAAATGACTGCATTTAAGACACTTTG
TTTGACGAAATCTAAAACAA

Product: oxidoreductase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKAVTYQGIKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGHEPMGIVEEVGSGVTKLKKGD
RVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYSGQTGGYPGGQAEYLRVPFANFTHFKIPESCEEPDEKLSVI
ADAMTTGFWSVDNAGVKKGDTVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEIVNFEDHENTGNYL
KEITKGGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVYGGRYNGFPLGDIMQRNVNIR
SGQAPVIHYMPYMFELVSTGKIDPGDVVSHVLPLSEAKHGYDIFDSKMDDCIKVVLKP

Sequences:

>Translated_378_residues
MKAVTYQGIKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGHEPMGIVEEVGSGVTKLKKGD
RVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYSGQTGGYPGGQAEYLRVPFANFTHFKIPESCEEPDEKLSVI
ADAMTTGFWSVDNAGVKKGDTVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEIVNFEDHENTGNYL
KEITKGGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVYGGRYNGFPLGDIMQRNVNIR
SGQAPVIHYMPYMFELVSTGKIDPGDVVSHVLPLSEAKHGYDIFDSKMDDCIKVVLKP
>Mature_378_residues
MKAVTYQGIKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGHEPMGIVEEVGSGVTKLKKGD
RVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYSGQTGGYPGGQAEYLRVPFANFTHFKIPESCEEPDEKLSVI
ADAMTTGFWSVDNAGVKKGDTVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEIVNFEDHENTGNYL
KEITKGGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVYGGRYNGFPLGDIMQRNVNIR
SGQAPVIHYMPYMFELVSTGKIDPGDVVSHVLPLSEAKHGYDIFDSKMDDCIKVVLKP

Specific function: Unknown

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily

Homologues:

Organism=Homo sapiens, GI34577061, Length=382, Percent_Identity=28.5340314136126, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4501929, Length=360, Percent_Identity=28.8888888888889, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI4501933, Length=356, Percent_Identity=28.0898876404494, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI71743840, Length=353, Percent_Identity=26.628895184136, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI71565152, Length=376, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI262073058, Length=353, Percent_Identity=26.628895184136, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI71565154, Length=265, Percent_Identity=31.3207547169811, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=26.0162601626016, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4501939, Length=257, Percent_Identity=29.1828793774319, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI156627571, Length=372, Percent_Identity=23.6559139784946, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1786825, Length=390, Percent_Identity=40.7692307692308, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI87082125, Length=368, Percent_Identity=26.3586956521739, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788073, Length=377, Percent_Identity=26.790450928382, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787863, Length=372, Percent_Identity=25.5376344086022, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790045, Length=315, Percent_Identity=28.8888888888889, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786552, Length=364, Percent_Identity=27.1978021978022, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI226510992, Length=363, Percent_Identity=23.1404958677686, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788407, Length=231, Percent_Identity=30.7359307359307, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788075, Length=373, Percent_Identity=24.1286863270777, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790718, Length=337, Percent_Identity=22.2551928783383, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=383, Percent_Identity=25.8485639686684, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25146526, Length=248, Percent_Identity=31.0483870967742, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71997431, Length=242, Percent_Identity=30.9917355371901, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17565904, Length=235, Percent_Identity=27.6595744680851, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=223, Percent_Identity=28.6995515695067, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=400, Percent_Identity=26.75, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319258, Length=265, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=266, Percent_Identity=29.3233082706767, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=393, Percent_Identity=25.1908396946565, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=393, Percent_Identity=25.1908396946565, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=339, Percent_Identity=25.0737463126844, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319257, Length=371, Percent_Identity=23.989218328841, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=243, Percent_Identity=27.5720164609054, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323980, Length=173, Percent_Identity=32.3699421965318, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17737895, Length=377, Percent_Identity=28.9124668435013, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137530, Length=379, Percent_Identity=26.6490765171504, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17737897, Length=378, Percent_Identity=25.3968253968254, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221457811, Length=365, Percent_Identity=24.3835616438356, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45551930, Length=365, Percent_Identity=24.3835616438356, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550770, Length=365, Percent_Identity=24.3835616438356, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADHB_BACSU (O06012)

Other databases:

- EMBL:   X92868
- EMBL:   AL009126
- PIR:   D69583
- RefSeq:   NP_390574.2
- HSSP:   P46154
- ProteinModelPortal:   O06012
- SMR:   O06012
- EnsemblBacteria:   EBBACT00000003017
- GeneID:   937604
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU26970
- NMPDR:   fig|224308.1.peg.2699
- GenoList:   BSU26970
- GeneTree:   EBGT00050000000383
- HOGENOM:   HBG753318
- ProtClustDB:   CLSK887645
- BioCyc:   BSUB:BSU26970-MONOMER
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11695

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N; SSF50129 GroES_like

EC number: 1.1.1.1; 1.2.1.1

Molecular weight: Translated: 41209; Mature: 41209

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVTYQGIKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGH
CCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEECCEECCCCHHHHHHHCCCCCCCEEECC
EPMGIVEEVGSGVTKLKKGDRVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYS
CCCHHHHHHCCCHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCC
GQTGGYPGGQAEYLRVPFANFTHFKIPESCEEPDEKLSVIADAMTTGFWSVDNAGVKKGD
CCCCCCCCCCCCEEEECCCCCEEEECCHHHCCHHHHHHHHHHHHHHCCEECCCCCCCCCC
TVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEIVNFEDHENTGNYL
EEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
KEITKGGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVY
HHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCEEEEEEE
GGRYNGFPLGDIMQRNVNIRSGQAPVIHYMPYMFELVSTGKIDPGDVVSHVLPLSEAKHG
CCCCCCCCHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
YDIFDSKMDDCIKVVLKP
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAVTYQGIKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGH
CCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEECCEECCCCHHHHHHHCCCCCCCEEECC
EPMGIVEEVGSGVTKLKKGDRVIIPFNIACGECFFCKNQLESQCDQSNDNGEMGAYFGYS
CCCHHHHHHCCCHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCC
GQTGGYPGGQAEYLRVPFANFTHFKIPESCEEPDEKLSVIADAMTTGFWSVDNAGVKKGD
CCCCCCCCCCCCEEEECCCCCEEEECCHHHCCHHHHHHHHHHHHHHCCEECCCCCCCCCC
TVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEIVNFEDHENTGNYL
EEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
KEITKGGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVRKGGTIQITGVY
HHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCEEEEEEE
GGRYNGFPLGDIMQRNVNIRSGQAPVIHYMPYMFELVSTGKIDPGDVVSHVLPLSEAKHG
CCCCCCCCHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
YDIFDSKMDDCIKVVLKP
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9141695; 9384377