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The map label for this gene is hemN

Identifier: 255767587

GI number: 255767587

Start: 2629718

End: 2630857

Strand: Reverse

Name: hemN

Synonym: BSU25500

Alternate gene names: 255767587

Gene position: 2630857-2629718 (Counterclockwise)

Preceding gene: 16079605

Following gene: 16079603

Centisome position: 62.41

GC content: 42.72

Gene sequence:

>1140_bases
TTGAAATCAGCTTATATCCATATCCCATTTTGTGAGCATATTTGCCACTACTGCGATTTCAATAAATATTTTATTCAAAG
TCAGCCAGTCGACGAGTATTTAAACGCACTCGAACAGGAAATGATAAATACAATAGCAAAAACCGGGCAGCCTGATCTCA
AAACAATCTTTATCGGAGGAGGCACGCCGACCTCCCTGTCTGAGGAACAGCTTAAAAAGCTGATGGATATGATCAACCGC
GTGCTAAAGCCCTCAAGCGACTTATCGGAATTTGCGGTTGAAGCGAATCCGGACGATTTGTCCGCAGAGAAATTAAAAAT
ATTAAAAGAAGCAGGCGTAAATCGCCTTAGCTTTGGTGTGCAAACGTTCGAAGACGATCTGTTAGAAAAAATCGGCCGAG
TGCACAAGCAAAAAGATGTATTCACCTCTTTTGAAAGAGCGCGGGAAATCGGTTTTGAGAATATCAGTCTTGATCTCATG
TTCGGGCTTCCGGGGCAAACCCTCAAGCATCTGGAACATTCCATAAACACTGCTTTATCATTGGACGCTGAGCATTATTC
CGTTTACTCCCTTATTGTAGAACCGAAAACGGTATTTTATAATTTGATGCAAAAAGGGCGGCTTCATCTTCCGCCGCAGG
AGCAGGAAGCTGAAATGTATGAAATAGTGATGAGCAAGATGGAAGCTCACGGCATTCATCAATATGAAATCAGCAACTTT
GCTAAGGCAGGCATGGAATCTAAGCACAATCTAACGTATTGGAGCAACGAACAATATTTCGGTTTCGGAGCTGGCGCTCA
CGGATATATTGGCGGCACACGAACAGTGAACGTCGGACCTGTTAAGCATTACATTGACCTGATAGCTGAAAAGGGTTTTC
CGTACAGAGATACACATGAAGTGACAACAGAAGAACAGATTGAGGAAGAAATGTTTTTAGGTTTGAGAAAAACAGCCGGT
GTCAGCAAAAAACGATTCGCAGAAAAATACGGCCGTTCCCTGGATGGACTTTTCCCAAGCGTGTTAAAAGACCTCGCTGA
AAAAGGGCTGATTCACAATTCGGAGTCTGCCGTTTATTTGACTCATCAAGGGAAATTATTAGGCAATGAAGTTTTTGGCG
CTTTTTTGGGTGAGTTATAA

Upstream 100 bases:

>100_bases
GCATTTATGGCAGTTCTGAAAATGGACGACAGTCCGAAAAAACAATAGAAGCCGCCGCAGTCTTGTACTGCGGCTTCTTT
CTGTAAAGAAGGTGAATCCG

Downstream 100 bases:

>100_bases
TTGACATTTTTCTTGTGGTTTGATACTTTTGTTATAGAATTAGCACTCGCTTATTGAGAGTGCTAACAGAGGTGATGATG
ATGTTAACAAATCGTCAGCT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKSAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGGGTPTSLSEEQLKKLMDMINR
VLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGVQTFEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLM
FGLPGQTLKHLEHSINTALSLDAEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNF
AKAGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVGPVKHYIDLIAEKGFPYRDTHEVTTEEQIEEEMFLGLRKTAG
VSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSESAVYLTHQGKLLGNEVFGAFLGEL

Sequences:

>Translated_379_residues
MKSAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGGGTPTSLSEEQLKKLMDMINR
VLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGVQTFEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLM
FGLPGQTLKHLEHSINTALSLDAEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNF
AKAGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVGPVKHYIDLIAEKGFPYRDTHEVTTEEQIEEEMFLGLRKTAG
VSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSESAVYLTHQGKLLGNEVFGAFLGEL
>Mature_379_residues
MKSAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGGGTPTSLSEEQLKKLMDMINR
VLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGVQTFEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLM
FGLPGQTLKHLEHSINTALSLDAEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNF
AKAGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVGPVKHYIDLIAEKGFPYRDTHEVTTEEQIEEEMFLGLRKTAG
VSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSESAVYLTHQGKLLGNEVFGAFLGEL

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI8922911, Length=264, Percent_Identity=35.9848484848485, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1789325, Length=381, Percent_Identity=32.2834645669291, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87082341, Length=394, Percent_Identity=26.3959390862944, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMN_BACSU (P54304)

Other databases:

- EMBL:   X91655
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   M84964
- PIR:   B69640
- RefSeq:   NP_390428.2
- ProteinModelPortal:   P54304
- SMR:   P54304
- EnsemblBacteria:   EBBACT00000003040
- GeneID:   937845
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25500
- NMPDR:   fig|224308.1.peg.2553
- GenoList:   BSU25500
- GeneTree:   EBGT00050000002687
- HOGENOM:   HBG529648
- PhylomeDB:   P54304
- ProtClustDB:   PRK08599
- BioCyc:   BSUB:BSU25500-MONOMER
- BRENDA:   1.3.99.22
- GO:   GO:0005737
- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197
- SMART:   SM00729
- TIGRFAMs:   TIGR00539

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM

EC number: =1.3.99.22

Molecular weight: Translated: 42888; Mature: 42888

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: BINDING 5-5 BINDING 17-17 BINDING 60-60 BINDING 94-94 BINDING 121-121 BINDING 133-133 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGG
CCCCEEECCHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
GTPTSLSEEQLKKLMDMINRVLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGV
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHCCCCHHHHHH
QTFEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLMFGLPGQTLKHLEHSINTALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHEE
LDAEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNF
CCCHHEEEEHEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
AKAGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVGPVKHYIDLIAEKGFPYRDTHE
HHHCCCCCCCCEEECCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCC
VTTEEQIEEEMFLGLRKTAGVSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSESAVYL
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCEEEE
THQGKLLGNEVFGAFLGEL
EECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGG
CCCCEEECCHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
GTPTSLSEEQLKKLMDMINRVLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGV
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHCCCCHHHHHH
QTFEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLMFGLPGQTLKHLEHSINTALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHEE
LDAEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNF
CCCHHEEEEHEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
AKAGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVGPVKHYIDLIAEKGFPYRDTHE
HHHCCCCCCCCEEECCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCC
VTTEEQIEEEMFLGLRKTAGVSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSESAVYL
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCEEEE
THQGKLLGNEVFGAFLGEL
EECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421