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The map label for this gene is glyS

Identifier: 255767576

GI number: 255767576

Start: 2605730

End: 2607769

Strand: Reverse

Name: glyS

Synonym: BSU25260

Alternate gene names: 255767576

Gene position: 2607769-2605730 (Counterclockwise)

Preceding gene: 16079581

Following gene: 16079579

Centisome position: 61.86

GC content: 44.22

Gene sequence:

>2040_bases
ATGAGTAAACAGGATTTACTTTTAGAGATCGGATTAGAGGAAATGCCGGCGCGCTTTTTGAATGAAAGCATGGTTCAGCT
TGGCGACAAGCTGACAGGCTGGCTTAAAGAAAAAAATATTACTCACGGTGAAGTGAAACTCTTCAATACACCGAGACGTC
TTGCTGTGTTCGTTAAGGATGTCGCAGAAAAGCAGGATGATATAAAAGAAGAAGCAAAAGGGCCTGCGAAAAAAATTGCC
CTTGATGCGGACGGGAACTGGACAAAAGCGGCAATCGGCTTTTCAAAAGGCCAAGGCGCAAATGTGGAAGACCTGTACAT
CAAAGAAGTAAAAGGCATAGAGTATGTATTCGTGCAAAAATTCCAAGCCGGCCAAGAAACGAAGTCGCTTCTGCCAGAAT
TGAGCGGTTTAATTACAAGCTTGCATTTCCCGAAAAACATGCGCTGGGGAAATGAAGATTTGCGTTATATCCGACCGATC
AAATGGATTGTCGCATTATTTGGACAGGATGTCATTCCATTTTCGATCACAAACGTTGAGTCTGGACGGACAACACAGGG
ACACCGTTTCCTCGGACATGAAGTGTCAATTGAATCACCTTCAGCTTATGAAGAGCAGCTCAAAGGACAGCATGTTATTG
CTGATCCTAGCGTCCGAAAGCAAATGATTCAATCTCAGCTGGAAACAATGGCTGCTGAAAACAATTGGAGCATTCCAGTT
GACGAAGACCTTCTTGATGAAGTGAATCATTTAGTAGAATACCCGACAGCTCTTTACGGTTCATTTGAATCCGAGTTTCT
GTCAATTCCTGAAGAAGTGCTTGTCACGACAATGAAAGAGCATCAGCGCTACTTCCCTGTCAAAGACAAAAACGGTGACC
TGCTGCCTCACTTTATCACAGTCCGAAACGGCAACAGCCACGCGATTGAAAATGTGGCCCGAGGCAATGAAAAAGTGCTG
CGCGCACGTTTATCTGATGCTTCATTCTTCTACAAAGAAGATCAGAAACTAAACATTGATGCAAATGTGAAGAAGCTTGA
AAATATCGTTTTCCATGAAGAGCTTGGTTCTCTTGCTGATAAGGTTAGGAGAGTCACATCAATCGCAGAGAAACTTGCCG
TTCGTCTTCAGGCTGATGAAGATACATTAAAACATGTGAAGCGGGCTGCTGAAATTTCTAAATTCGACCTTGTGACGCAT
ATGATATATGAATTCCCTGAACTTCAAGGAATTATGGGCGAAAAGTATGCGAGAATGCTTGGCGAAGATGAAGCTGTGGC
TGCGGCAGTTAATGAACATTATATGCCTAGATCAGCGGGCGGAGAAACGCCTTCTACTTTCACCGGAGCTGTTGTAGCAA
TGGCGGATAAGCTTGACACAATCGCTTCATTCTTCTCTATCGGCGTAATTCCGACCGGTTCCCAGGACCCTTACGGACTG
CGCCGCCAAGCAAGCGGTATTGTTGCGATTCTTCTTGACCGCAACTGGGGAATTTCATTTGAAGAGCTGCTTACTTTCGT
TCAAACCGATAAAGAAAATGAACTGCTGGATTTCTTCACTCAGCGCTTGAAATATGTCTTGAATGCTGAACAAATCAGAC
ATGATGTCATTGACGCCGTGCTGGAAAGCTCTGAGCTTGAGCCGTACTCTGCGCTGCATAAAGCACAAGTACTGGAGCAA
AAGCTTGGTGCACCTGGCTTTAAGGAAACAGCCGAAGCGTTAGGCCGAGTGATCTCTATCAGCAAAAAAGGAGTCCGCGG
AGACATTCAGCCTGATCTGTTTGAAAATGAATACGAAGCAAAACTGTTTGATGCCTACCAAACAGCGAAGCAAAATCTGC
AGGAAAACTTCAGCAAAAAAGATTATGAGGCGGCGCTTGCTTCACTTGCAGCCTTAAAAGAACCGATCGATGCTTACTTC
GATCATACAATGGTTATAGCGGATAATGAGTCATTAAAGGCAAATCGTTTAGCGCAAATGGTAAGCTTGGCGGATGAGAT
CAAGTCCTTTGCGAATATGAATGCCCTTATTGTAAAATAA

Upstream 100 bases:

>100_bases
ATCGCAAGAGTGCGGAATTTAGCTAGAAAAGTAGCAAAAACCTACTATGAGGAACGAGAAAAACTAGGGTTCCCAATGCT
TAAAGGGGAGGGTTCTTCTC

Downstream 100 bases:

>100_bases
AGAGAGAAAAGGCGGTTGCTGTGTGATCGGTAATCGTCTTTTCCCCATTTATAAGTTTTTTGTTTGATTACAAGATATTT
ACGTTATAATATGGATTGTT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSKQDLLLEIGLEEMPARFLNESMVQLGDKLTGWLKEKNITHGEVKLFNTPRRLAVFVKDVAEKQDDIKEEAKGPAKKIA
LDADGNWTKAAIGFSKGQGANVEDLYIKEVKGIEYVFVQKFQAGQETKSLLPELSGLITSLHFPKNMRWGNEDLRYIRPI
KWIVALFGQDVIPFSITNVESGRTTQGHRFLGHEVSIESPSAYEEQLKGQHVIADPSVRKQMIQSQLETMAAENNWSIPV
DEDLLDEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDKNGDLLPHFITVRNGNSHAIENVARGNEKVL
RARLSDASFFYKEDQKLNIDANVKKLENIVFHEELGSLADKVRRVTSIAEKLAVRLQADEDTLKHVKRAAEISKFDLVTH
MIYEFPELQGIMGEKYARMLGEDEAVAAAVNEHYMPRSAGGETPSTFTGAVVAMADKLDTIASFFSIGVIPTGSQDPYGL
RRQASGIVAILLDRNWGISFEELLTFVQTDKENELLDFFTQRLKYVLNAEQIRHDVIDAVLESSELEPYSALHKAQVLEQ
KLGAPGFKETAEALGRVISISKKGVRGDIQPDLFENEYEAKLFDAYQTAKQNLQENFSKKDYEAALASLAALKEPIDAYF
DHTMVIADNESLKANRLAQMVSLADEIKSFANMNALIVK

Sequences:

>Translated_679_residues
MSKQDLLLEIGLEEMPARFLNESMVQLGDKLTGWLKEKNITHGEVKLFNTPRRLAVFVKDVAEKQDDIKEEAKGPAKKIA
LDADGNWTKAAIGFSKGQGANVEDLYIKEVKGIEYVFVQKFQAGQETKSLLPELSGLITSLHFPKNMRWGNEDLRYIRPI
KWIVALFGQDVIPFSITNVESGRTTQGHRFLGHEVSIESPSAYEEQLKGQHVIADPSVRKQMIQSQLETMAAENNWSIPV
DEDLLDEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDKNGDLLPHFITVRNGNSHAIENVARGNEKVL
RARLSDASFFYKEDQKLNIDANVKKLENIVFHEELGSLADKVRRVTSIAEKLAVRLQADEDTLKHVKRAAEISKFDLVTH
MIYEFPELQGIMGEKYARMLGEDEAVAAAVNEHYMPRSAGGETPSTFTGAVVAMADKLDTIASFFSIGVIPTGSQDPYGL
RRQASGIVAILLDRNWGISFEELLTFVQTDKENELLDFFTQRLKYVLNAEQIRHDVIDAVLESSELEPYSALHKAQVLEQ
KLGAPGFKETAEALGRVISISKKGVRGDIQPDLFENEYEAKLFDAYQTAKQNLQENFSKKDYEAALASLAALKEPIDAYF
DHTMVIADNESLKANRLAQMVSLADEIKSFANMNALIVK
>Mature_678_residues
SKQDLLLEIGLEEMPARFLNESMVQLGDKLTGWLKEKNITHGEVKLFNTPRRLAVFVKDVAEKQDDIKEEAKGPAKKIAL
DADGNWTKAAIGFSKGQGANVEDLYIKEVKGIEYVFVQKFQAGQETKSLLPELSGLITSLHFPKNMRWGNEDLRYIRPIK
WIVALFGQDVIPFSITNVESGRTTQGHRFLGHEVSIESPSAYEEQLKGQHVIADPSVRKQMIQSQLETMAAENNWSIPVD
EDLLDEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDKNGDLLPHFITVRNGNSHAIENVARGNEKVLR
ARLSDASFFYKEDQKLNIDANVKKLENIVFHEELGSLADKVRRVTSIAEKLAVRLQADEDTLKHVKRAAEISKFDLVTHM
IYEFPELQGIMGEKYARMLGEDEAVAAAVNEHYMPRSAGGETPSTFTGAVVAMADKLDTIASFFSIGVIPTGSQDPYGLR
RQASGIVAILLDRNWGISFEELLTFVQTDKENELLDFFTQRLKYVLNAEQIRHDVIDAVLESSELEPYSALHKAQVLEQK
LGAPGFKETAEALGRVISISKKGVRGDIQPDLFENEYEAKLFDAYQTAKQNLQENFSKKDYEAALASLAALKEPIDAYFD
HTMVIADNESLKANRLAQMVSLADEIKSFANMNALIVK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=35.4558610709117, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_BACSU (P54381)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B69636
- RefSeq:   NP_390404.2
- ProteinModelPortal:   P54381
- EnsemblBacteria:   EBBACT00000001733
- GeneID:   937887
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25260
- NMPDR:   fig|224308.1.peg.2529
- GenoList:   BSU25260
- GeneTree:   EBGT00050000002728
- HOGENOM:   HBG586599
- PhylomeDB:   P54381
- ProtClustDB:   PRK01233
- BioCyc:   BSUB:BSU25260-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 76292; Mature: 76160

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQDLLLEIGLEEMPARFLNESMVQLGDKLTGWLKEKNITHGEVKLFNTPRRLAVFVKD
CCCCCEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHH
VAEKQDDIKEEAKGPAKKIALDADGNWTKAAIGFSKGQGANVEDLYIKEVKGIEYVFVQK
HHHHHHHHHHHHCCCHHEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHH
FQAGQETKSLLPELSGLITSLHFPKNMRWGNEDLRYIRPIKWIVALFGQDVIPFSITNVE
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
SGRTTQGHRFLGHEVSIESPSAYEEQLKGQHVIADPSVRKQMIQSQLETMAAENNWSIPV
CCCCCCCCCCCCCEECCCCCCHHHHHHCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCC
DEDLLDEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDKNGDLLPHFIT
CHHHHHHHHHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VRNGNSHAIENVARGNEKVLRARLSDASFFYKEDQKLNIDANVKKLENIVFHEELGSLAD
EECCCCHHHHHHHCCCHHHHHHHHCCCHHEECCCCEEECCCCHHHHHHHHHHHHHHHHHH
KVRRVTSIAEKLAVRLQADEDTLKHVKRAAEISKFDLVTHMIYEFPELQGIMGEKYARML
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEDEAVAAAVNEHYMPRSAGGETPSTFTGAVVAMADKLDTIASFFSIGVIPTGSQDPYGL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCH
RRQASGIVAILLDRNWGISFEELLTFVQTDKENELLDFFTQRLKYVLNAEQIRHDVIDAV
HHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESSELEPYSALHKAQVLEQKLGAPGFKETAEALGRVISISKKGVRGDIQPDLFENEYEA
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHH
KLFDAYQTAKQNLQENFSKKDYEAALASLAALKEPIDAYFDHTMVIADNESLKANRLAQM
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHH
VSLADEIKSFANMNALIVK
HHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
SKQDLLLEIGLEEMPARFLNESMVQLGDKLTGWLKEKNITHGEVKLFNTPRRLAVFVKD
CCCCEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHH
VAEKQDDIKEEAKGPAKKIALDADGNWTKAAIGFSKGQGANVEDLYIKEVKGIEYVFVQK
HHHHHHHHHHHHCCCHHEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHH
FQAGQETKSLLPELSGLITSLHFPKNMRWGNEDLRYIRPIKWIVALFGQDVIPFSITNVE
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
SGRTTQGHRFLGHEVSIESPSAYEEQLKGQHVIADPSVRKQMIQSQLETMAAENNWSIPV
CCCCCCCCCCCCCEECCCCCCHHHHHHCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCC
DEDLLDEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDKNGDLLPHFIT
CHHHHHHHHHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VRNGNSHAIENVARGNEKVLRARLSDASFFYKEDQKLNIDANVKKLENIVFHEELGSLAD
EECCCCHHHHHHHCCCHHHHHHHHCCCHHEECCCCEEECCCCHHHHHHHHHHHHHHHHHH
KVRRVTSIAEKLAVRLQADEDTLKHVKRAAEISKFDLVTHMIYEFPELQGIMGEKYARML
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEDEAVAAAVNEHYMPRSAGGETPSTFTGAVVAMADKLDTIASFFSIGVIPTGSQDPYGL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCH
RRQASGIVAILLDRNWGISFEELLTFVQTDKENELLDFFTQRLKYVLNAEQIRHDVIDAV
HHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESSELEPYSALHKAQVLEQKLGAPGFKETAEALGRVISISKKGVRGDIQPDLFENEYEA
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHH
KLFDAYQTAKQNLQENFSKKDYEAALASLAALKEPIDAYFDHTMVIADNESLKANRLAQM
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHH
VSLADEIKSFANMNALIVK
HHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377