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The map label for this gene is ansA

Identifier: 16079415

GI number: 16079415

Start: 2455819

End: 2456808

Strand: Reverse

Name: ansA

Synonym: BSU23580

Alternate gene names: 16079415

Gene position: 2456808-2455819 (Counterclockwise)

Preceding gene: 255767513

Following gene: 255767512

Centisome position: 58.28

GC content: 43.64

Gene sequence:

>990_bases
ATGAAAAAATTATTGATGTTGACAACTGGGGGAACGATTGCTTCAGTTGAAGGGGAAAATGGGCTGGCTCCCGGAGTCAA
GGCTGATGAATTATTAAGTTACGTATCAAAACTTGATAACGATTACACAATGGAAACTCAGTCGCTTATGAATATAGACA
GCACCAATATGCAGCCTGAATACTGGGTGGAAATAGCAGAAGCCGTTAAGGAAAATTATGATGCCTATGACGGGTTTGTT
ATTACTCACGGTACAGATACAATGGCCTATACATCTGCCGCACTATCGTATATGCTGCAGCATGCCAAAAAGCCGATTGT
CATCACCGGCTCGCAGATTCCGATCACGTTCCAAAAAACCGATGCCAAAAAAAATATTACAGATGCCATTCGATTTGCCT
GTGAAGGCGTGGGCGGCGTTTATGTTGTGTTTGACGGCAGAGTCATTCAGGGAACGCGTGCGATCAAATTAAGAACGAAA
AGCTACGACGCATTTGAAAGCATCAATTACCCATATATCGCTTTTATCAATGAAGACGGGATCGAATACAACAAACAAGT
AACGGAACCTGAGAACGACACCTTCACAGTTGACACTTCACTATGCACAGATGTATGTCTGCTGAAGCTGCATCCAGGCT
TAAAGCCCGAAATGTTTGATGCCCTGAAAAGCATGTACAAAGGAATTGTCATTGAGAGTTATGGCAGCGGAGGAGTGCCG
TTTGAAGGCAGAGACATTTTGTCAAAAGTGAATGAACTGATCGAAAGCGGCATTGTCGTCGTCATTACGACTCAATGTCT
TGAAGAAGGCGAAGACATGAGCATTTACGAGGTTGGCCGCAGAGTCAACCAAGACTTAATTATCCGATCAAGAAATATGA
ACACAGAAGCAATTGTGCCAAAATTGATGTGGGCGCTAGGTCAGTCTTCGGATCTTCCTGTCGTCAAGAGAATTATGGAA
ACGCCGATAGCTGATGACGTTGTCCTGTAA

Upstream 100 bases:

>100_bases
TGCGTATTTTATTTTCCGTGTCTATAATTGCATTAAAAGTATGCGAAAAGCGAATACCTTCTCGCAACCCATCTTTAGAT
ACAGTGAAGAGGTGCATGGT

Downstream 100 bases:

>100_bases
AATGTAAAAACGATAACCAGCAAAGAGAAAGAAGGTTAAAGATTATGTTAAACGGCCAAAAAGAATATCGCGTGGAAAAA
GACTTCCTTGGGGAAAAACA

Product: exported L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKLLMLTTGGTIASVEGENGLAPGVKADELLSYVSKLDNDYTMETQSLMNIDSTNMQPEYWVEIAEAVKENYDAYDGFV
ITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKTDAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTK
SYDAFESINYPYIAFINEDGIEYNKQVTEPENDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSMYKGIVIESYGSGGVP
FEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSSDLPVVKRIME
TPIADDVVL

Sequences:

>Translated_329_residues
MKKLLMLTTGGTIASVEGENGLAPGVKADELLSYVSKLDNDYTMETQSLMNIDSTNMQPEYWVEIAEAVKENYDAYDGFV
ITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKTDAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTK
SYDAFESINYPYIAFINEDGIEYNKQVTEPENDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSMYKGIVIESYGSGGVP
FEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSSDLPVVKRIME
TPIADDVVL
>Mature_329_residues
MKKLLMLTTGGTIASVEGENGLAPGVKADELLSYVSKLDNDYTMETQSLMNIDSTNMQPEYWVEIAEAVKENYDAYDGFV
ITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKTDAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTK
SYDAFESINYPYIAFINEDGIEYNKQVTEPENDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSMYKGIVIESYGSGGVP
FEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSSDLPVVKRIME
TPIADDVVL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family

Homologues:

Organism=Homo sapiens, GI122937331, Length=342, Percent_Identity=30.7017543859649, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1788065, Length=328, Percent_Identity=32.3170731707317, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789327, Length=163, Percent_Identity=30.6748466257669, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=274, Percent_Identity=33.5766423357664, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI193207372, Length=274, Percent_Identity=33.5766423357664, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI193207374, Length=274, Percent_Identity=33.5766423357664, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17558278, Length=274, Percent_Identity=33.5766423357664, Blast_Score=167, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6320527, Length=264, Percent_Identity=28.030303030303, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=27.2401433691756, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=27.2401433691756, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=27.2401433691756, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=27.2401433691756, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24645530, Length=350, Percent_Identity=32, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24639609, Length=276, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPG1_BACSU (P26900)

Other databases:

- EMBL:   M63264
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   L08205
- PIR:   A39440
- RefSeq:   NP_390239.1
- ProteinModelPortal:   P26900
- SMR:   P26900
- EnsemblBacteria:   EBBACT00000002870
- GeneID:   938722
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23580
- NMPDR:   fig|224308.1.peg.2363
- GenoList:   BSU23580
- GeneTree:   EBGT00050000000510
- HOGENOM:   HBG497495
- OMA:   DMNTEAI
- PhylomeDB:   P26900
- ProtClustDB:   CLSK887522
- BioCyc:   BSUB:BSU23580-MONOMER
- BRENDA:   3.5.1.1
- GO:   GO:0005737
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: =3.5.1.1

Molecular weight: Translated: 36455; Mature: 36455

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 12-12 BINDING 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLMLTTGGTIASVEGENGLAPGVKADELLSYVSKLDNDYTMETQSLMNIDSTNMQPE
CCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHCCCCCCCCHH
YWVEIAEAVKENYDAYDGFVITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECC
DAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFINEDG
CCCCHHHHHHHHHHCCCCCEEEEECCEEEECCEEEEEEECCCHHHHHCCCCEEEEECCCC
IEYNKQVTEPENDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSMYKGIVIESYGSGGVP
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
FEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVP
CCCHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHH
KLMWALGQSSDLPVVKRIMETPIADDVVL
HHHHHHCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKLLMLTTGGTIASVEGENGLAPGVKADELLSYVSKLDNDYTMETQSLMNIDSTNMQPE
CCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHCCCCCCCCHH
YWVEIAEAVKENYDAYDGFVITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECC
DAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFINEDG
CCCCHHHHHHHHHHCCCCCEEEEECCEEEECCEEEEEEECCCHHHHHCCCCEEEEECCCC
IEYNKQVTEPENDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSMYKGIVIESYGSGGVP
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
FEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVP
CCCHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHH
KLMWALGQSSDLPVVKRIMETPIADDVVL
HHHHHHCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318