Click here to switch to the map view.

The map label for this gene is sigX

Identifier: 255767497

GI number: 255767497

Start: 2414627

End: 2415211

Strand: Reverse

Name: sigX

Synonym: BSU23100

Alternate gene names: 255767497

Gene position: 2415211-2414627 (Counterclockwise)

Preceding gene: 16079368

Following gene: 255767496

Centisome position: 57.29

GC content: 40.17

Gene sequence:

>585_bases
ATGGAAGAAACCTTTCAATTATTATATGATACATATCATCAAGATTTGTACCAATTTTTATTTTATATGGTAAAGGATAA
AAACCAGACAGAAGACCTTCTTCAAGAGGTTTATATCCGGGTATTAAATTCTTATCATACGTTTGAAGGAAGAAGCAGTG
AAAAAACATGGCTTTTGTCTATCGCACGCCATGTTGCGATAGACTGGTTCCGTAAACAGCAAACAATCAGACAGCGTATA
CTCGGTACATTTGACTGGGATACACAGGATGTCAGGGATCAGCAATTGCTGCCGGACGAACTTGCGGTTCAACATGAAAA
TGTCCGGGAAATTTATGCTGCGCTTGATCAATGTACAATCGATCAAAGAGCTGTGATTATCTTGCGGTTTATTCAGGGAT
ATTCAATACAGGAAACAGCCAAGGCTCTGCGCTTCTCGGAAAGCAAAGTAAAAACGACCCAACACAGAGGGCTCAAAGTA
CTTCGGAAACATATGGAGCTTTTGAGGGAGGAGCTAATGGATGATGAAGTCAGAATGGAACGAAGAACAGATAAAGGAGT
TGTTAAGTCAACTTCCGGCAGTTAA

Upstream 100 bases:

>100_bases
TAATATAAATTCCCTTCCAAATTCCAGTTACTCGTAATATAGTTGTAATGTAACTTTTCAAGCTATTCATACGACAAAAA
AGTGAACGGAGGGGTTTCAA

Downstream 100 bases:

>100_bases
AGACCATCGTTCGCCTCAGGATATTTACAAGCGGCTGACGATGGCAAAGCGTAAAAATAAGCCCGCTGTCAGGTGGATTG
GCCCCGCGTGTGCCGCTGCA

Product: RNA polymerase sigma factor SigX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGRSSEKTWLLSIARHVAIDWFRKQQTIRQRI
LGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTIDQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKV
LRKHMELLREELMDDEVRMERRTDKGVVKSTSGS

Sequences:

>Translated_194_residues
MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGRSSEKTWLLSIARHVAIDWFRKQQTIRQRI
LGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTIDQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKV
LRKHMELLREELMDDEVRMERRTDKGVVKSTSGS
>Mature_194_residues
MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGRSSEKTWLLSIARHVAIDWFRKQQTIRQRI
LGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTIDQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKV
LRKHMELLREELMDDEVRMERRTDKGVVKSTSGS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. May be involved in the regulation of iron metabolism

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SIGX_BACSU (P35165)

Other databases:

- EMBL:   L09228
- EMBL:   AL009126
- PIR:   S45561
- RefSeq:   NP_390191.2
- ProteinModelPortal:   P35165
- SMR:   P35165
- IntAct:   P35165
- EnsemblBacteria:   EBBACT00000001412
- GeneID:   938966
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23100
- NMPDR:   fig|224308.1.peg.2314
- GenoList:   BSU23100
- GeneTree:   EBGT00070000031727
- HOGENOM:   HBG753452
- ProtClustDB:   PRK09639
- BioCyc:   BSUB:BSU23100-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 23224; Mature: 23224

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGRSSEKTWLLS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
IARHVAIDWFRKQQTIRQRILGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
DQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKVLRKHMELLREELMDDEVRME
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RRTDKGVVKSTSGS
HHHCCCCCCCCCCC
>Mature Secondary Structure
MEETFQLLYDTYHQDLYQFLFYMVKDKNQTEDLLQEVYIRVLNSYHTFEGRSSEKTWLLS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
IARHVAIDWFRKQQTIRQRILGTFDWDTQDVRDQQLLPDELAVQHENVREIYAALDQCTI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
DQRAVIILRFIQGYSIQETAKALRFSESKVKTTQHRGLKVLRKHMELLREELMDDEVRME
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RRTDKGVVKSTSGS
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 8052622; 9393439