Click here to switch to the map view.

The map label for this gene is gudB

Identifier: 255767494

GI number: 255767494

Start: 2402067

End: 2403350

Strand: Reverse

Name: gudB

Synonym: BSU22960

Alternate gene names: 255767494

Gene position: 2403350-2402067 (Counterclockwise)

Preceding gene: 16079354

Following gene: 255767493

Centisome position: 57.01

GC content: 44.47

Gene sequence:

>1284_bases
ATGGCAGCCGATCGAAACACCGGTCATACAGAAGAGGACAAACTTGATGTATTAAAATCAACCCAAACCGTAATACATAA
GGCTCTGGAAAAATTGGGATATCCCGAAGAGGTATACGAATTGTTAAAAGAGCCGATGAGATTATTAACGGTAAAAATAC
CTGTTCGTATGGACGACGGTTCAGTAAAGATTTTCACAGGATATCGTGCGCAGCACAATGACTCTGTCGGTCCAACGAAA
GGCGGGATACGTTTTCACCCGAACGTAACAGAAAAAGAGGTGAAGGCGGTGAAGGCGCTTTCAATTTGGATGAGTTTAAA
ATGCGGCATAATTGATCTTCCATATGGCGGTGGTAAAGGCGGAATTGTTTGTGATCCAAGGGATATGTCGTTTAGAGAGC
TGGAGCGTCTGAGCAGAGGGTATGTCAGAGCGATCAGCCAAATTGTCGGCCCGACAAAAGACGTGCCGGCACCGGATGTA
TTTACAAACTCACAAATCATGGCTTGGATGATGGATGAGTATTCAAGAATTGATGAATTTAATTCGCCTGGATTTATTAC
AGGCAAACCGCTTGTGCTTGGCGGATCTCACGGGAGAGAATCTGCGACAGCAAAAGGTGTTACCATCTGTATTAAAGAAG
CGGCTAAGAAGAGAGGCATCGATATTAAAGGTGCGCGTGTCGTTGTCCAAGGCTTCGGAAACGCGGGAAGCTATTTGGCA
AAATTTATGCATGATGCGGGGGCAAAAGTTGTCGGCATCTCAGATGCGTATGGCGGACTTTATGATCCGGAAGGCCTTGA
TATCGATTATTTACTCGACCGACGCGACAGCTTCGGTACCGTAACAAAGCTTTTCAACGATACCATTACCAACCAAGAGC
TGCTGGAGCTGGATTGTGATATTCTCGTTCCTGCTGCGATTGAAAATCAAATTACAGAAGAAAATGCCCATAATATCCGG
GCTAAAATTGTCGTTGAAGCAGCGAACGGACCAACAACGCTTGAAGGAACAAAAATTCTTTCAGACCGGGACATTCTGCT
TGTACCAGACGTGCTGGCAAGTGCCGGTGGCGTAACAGTTTCTTATTTTGAATGGGTTCAGAATAACCAAGGCTTCTACT
GGAGTGAAGAAGAGGTAGAAGAAAAATTAGAAAAAATGATGGTCAAATCATTTAACAATATTTACGAAATGGCTAACAAC
CGAAGAATTGACATGAGGCTCGCTGCATATATGGTCGGCGTTCGCAAAATGGCTGAAGCTTCGCGTTTTAGAGGCTGGAT
ATAA

Upstream 100 bases:

>100_bases
AAAACATAACGTTTATTCTATGCACAGATCATATGAAGGTGTATACTATTCTCTGAAAGGGAGCATGTGAGAAACAGAAG
ATTCTAGGAGGTTAACTCAA

Downstream 100 bases:

>100_bases
GTTGATGATTTGCATAAAAATAAAAAATCTCCTATGATAAAATAGGAGATTTTTTTATTCGGAAATAGAAAATATGAAGT
GAAATGCCAATCGCAGGAGT

Product: cryptic glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAADRNTGHTEEDKLDVLKSTQTVIHKALEKLGYPEEVYELLKEPMRLLTVKIPVRMDDGSVKIFTGYRAQHNDSVGPTK
GGIRFHPNVTEKEVKAVKALSIWMSLKCGIIDLPYGGGKGGIVCDPRDMSFRELERLSRGYVRAISQIVGPTKDVPAPDV
FTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKRGIDIKGARVVVQGFGNAGSYLA
KFMHDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILVPAAIENQITEENAHNIR
AKIVVEAANGPTTLEGTKILSDRDILLVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEVEEKLEKMMVKSFNNIYEMANN
RRIDMRLAAYMVGVRKMAEASRFRGWI

Sequences:

>Translated_427_residues
MAADRNTGHTEEDKLDVLKSTQTVIHKALEKLGYPEEVYELLKEPMRLLTVKIPVRMDDGSVKIFTGYRAQHNDSVGPTK
GGIRFHPNVTEKEVKAVKALSIWMSLKCGIIDLPYGGGKGGIVCDPRDMSFRELERLSRGYVRAISQIVGPTKDVPAPDV
FTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKRGIDIKGARVVVQGFGNAGSYLA
KFMHDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILVPAAIENQITEENAHNIR
AKIVVEAANGPTTLEGTKILSDRDILLVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEVEEKLEKMMVKSFNNIYEMANN
RRIDMRLAAYMVGVRKMAEASRFRGWI
>Mature_426_residues
AADRNTGHTEEDKLDVLKSTQTVIHKALEKLGYPEEVYELLKEPMRLLTVKIPVRMDDGSVKIFTGYRAQHNDSVGPTKG
GIRFHPNVTEKEVKAVKALSIWMSLKCGIIDLPYGGGKGGIVCDPRDMSFRELERLSRGYVRAISQIVGPTKDVPAPDVF
TNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKRGIDIKGARVVVQGFGNAGSYLAK
FMHDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILVPAAIENQITEENAHNIRA
KIVVEAANGPTTLEGTKILSDRDILLVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEVEEKLEKMMVKSFNNIYEMANNR
RIDMRLAAYMVGVRKMAEASRFRGWI

Specific function: The wild-type version has little or no activity

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=344, Percent_Identity=39.8255813953488, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI4885281, Length=344, Percent_Identity=39.5348837209302, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788059, Length=411, Percent_Identity=30.1703163017032, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=345, Percent_Identity=40.5797101449275, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6319256, Length=403, Percent_Identity=28.0397022332506, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324951, Length=411, Percent_Identity=27.7372262773723, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45549226, Length=343, Percent_Identity=42.2740524781341, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI45553475, Length=343, Percent_Identity=42.2740524781341, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24649283, Length=378, Percent_Identity=39.4179894179894, Blast_Score=257, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=41.9354838709677, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GUDB_BACSU (P50735)

Other databases:

- EMBL:   L47648
- EMBL:   AL009126
- PIR:   G69933
- RefSeq:   NP_390177.2
- PDB:   3K8Z
- PDBsum:   3K8Z
- ProteinModelPortal:   P50735
- SMR:   P50735
- EnsemblBacteria:   EBBACT00000002322
- GeneID:   938975
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22960
- NMPDR:   fig|224308.1.peg.2300
- GenoList:   BSU22960
- GeneTree:   EBGT00050000001222
- HOGENOM:   HBG590661
- ProtClustDB:   CLSK887504
- BioCyc:   BSUB:BSU22960-MONOMER
- BRENDA:   1.4.1.2
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.2

Molecular weight: Translated: 47341; Mature: 47210

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADRNTGHTEEDKLDVLKSTQTVIHKALEKLGYPEEVYELLKEPMRLLTVKIPVRMDDG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCC
SVKIFTGYRAQHNDSVGPTKGGIRFHPNVTEKEVKAVKALSIWMSLKCGIIDLPYGGGKG
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GIVCDPRDMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTNSQIMAWMMDEYSRIDEF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKRGIDIKGARVVVQGFGNAGSYLA
CCCCEECCCCEEEECCCCCCCCCCCCHHHEEHHHHHHCCCCCCCCEEEEEECCCHHHHHH
KFMHDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCD
HHHHHCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCC
ILVPAAIENQITEENAHNIRAKIVVEAANGPTTLEGTKILSDRDILLVPDVLASAGGVTV
EEECHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEECCCCEEEECHHHHCCCCCEE
SYFEWVQNNQGFYWSEEEVEEKLEKMMVKSFNNIYEMANNRRIDMRLAAYMVGVRKMAEA
EEHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
SRFRGWI
HHHCCCC
>Mature Secondary Structure 
AADRNTGHTEEDKLDVLKSTQTVIHKALEKLGYPEEVYELLKEPMRLLTVKIPVRMDDG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCC
SVKIFTGYRAQHNDSVGPTKGGIRFHPNVTEKEVKAVKALSIWMSLKCGIIDLPYGGGKG
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GIVCDPRDMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTNSQIMAWMMDEYSRIDEF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKRGIDIKGARVVVQGFGNAGSYLA
CCCCEECCCCEEEECCCCCCCCCCCCHHHEEHHHHHHCCCCCCCCEEEEEECCCHHHHHH
KFMHDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCD
HHHHHCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCC
ILVPAAIENQITEENAHNIRAKIVVEAANGPTTLEGTKILSDRDILLVPDVLASAGGVTV
EEECHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEECCCCEEEECHHHHCCCCCEE
SYFEWVQNNQGFYWSEEEVEEKLEKMMVKSFNNIYEMANNRRIDMRLAAYMVGVRKMAEA
EEHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
SRFRGWI
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940