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The map label for this gene is gltD

Identifier: 255767433

GI number: 255767433

Start: 2008572

End: 2010053

Strand: Reverse

Name: gltD

Synonym: BSU18440

Alternate gene names: 255767433

Gene position: 2010053-2008572 (Counterclockwise)

Preceding gene: 255767434

Following gene: 16078903

Centisome position: 47.68

GC content: 46.49

Gene sequence:

>1482_bases
ATGGGGAAACCAACTGGATTTATGGAGATCAAACGAGAAAAACCTGCGGAGCGGGACCCTCTCACTCGCTTAAAGGATTG
GAAAGAATATTCTGCTCCTTTTTCAGAAGAAGCATCGAAACGGCAAGGGGCGCGGTGTATGGATTGCGGTACACCGTTTT
GCCAGATCGGTGCGGACATTAACGGATTTACATCCGGCTGTCCGATTTACAACTTAATTCCTGAATGGAATGATCTTGTC
TACCGGGGCAGATGGAAGGAAGCATTGGAACGTCTGTTGAAAACAAACAACTTTCCTGAATTCACAGGGCGGGTATGCCC
CGCTCCTTGTGAAGGATCATGCACTTTAGCGATTTCAGATCCGGCCGTATCAATTAAAAACATCGAGCGGACCATTATCG
ATAAAGGATTTGAGAACGGCTGGATTCAACCTAGAATTCCAAAAAAACGAACAGGCAAAAAAGTGGCCATCGTCGGGTCA
GGCCCAGCCGGACTGGCGAGTGCTGACCAGCTGAATCAAGCGGGACATTCCGTCACTGTTTTTGAACGAGCAGACAGAGC
GGGCGGCCTTTTAACATACGGCATTCCAAACATGAAGCTTGAGAAGGGCATTGTAGAACGCCGAATTAAATTGCTGACGC
AAGAGGGAATTGATTTTGTCACGAACACAGAAATCGGTGTTGACATTACAGCTGATGAGCTAAAGGAGCAATTTGATGCT
GTGATCTTATGCACAGGCGCTCAAAAACAGCGGGACCTCTTAATCGAAGGACGCGACTCAAAAGGAGTCCATTACGCAAT
GGACTACCTCACACTTGCAACAAAAAGCTATTTAGATTCTAATTTTAAAGACAAGCAATTCATTGATGCCAAAGGGAAAG
ATGTCATCGTAATCGGAGGCGGAGATACAGGAGCTGACTGTGTGGCTACCGCTCTGCGTCAAAAAGCCAAAAGCGTGCAC
CAATTCGGCAAACACCCGAAACTTCCGCCAGCCCGCACGAATGACAATATGTGGCCTGAACAGCCACACGTCTTTACGCT
TGAATATGCATACGAGGAAGCAGAAGCAAAATTCGGAAGAGATCCGAGAGAATACTCCATTCAAACGACAAAAATGGTCG
CGGATAAAAACGGAAAGCTGAAAGAGCTTCATACCATCCAAATGGAAAAAGTGAAAAATGAGCATGGAAAATACGAGTTC
CGCGAGCTGCCTGGAACGGAAAAAGTATGGCCTGCTCAGCTTGTCTTTATCGCTATCGGCTTTGAAGGCACAGAACAGCC
GCTGTTGAAACAGTTCGGTGTTAATTCAGTAAATAACAAAATCAGTGCAGCATATGGGGATTATCAAACAAATATCGATG
GTGTATTCGCCGCAGGGGATGCAAGAAGAGGGCAAAGCTTAATCGTATGGGCGATTAATGAAGGCCGTGAAGTGGCGCGT
GAAGTGGATCGATATTTGATGGGGAGTTCAGTTCTTCCGTAA

Upstream 100 bases:

>100_bases
GCCATCATGTTCGCTTTTGAGGCCAACACGAAGCCAAAGCAGAATACAGCAGCATCGGGACAAAAACAAGCGGTAGTACA
GTAAGGAAGGGGAGAGGAAC

Downstream 100 bases:

>100_bases
ATAAAGGGGATTATCATATAAGAGAAACCGGTCTGGCTGCCAGCCGGTTTCTTTTTTTATTCCATTACGATCCCGATGTT
TCCAAACTGTCTGCCTTCCT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKPTGFMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTPFCQIGADINGFTSGCPIYNLIPEWNDLV
YRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPKKRTGKKVAIVGS
GPAGLASADQLNQAGHSVTVFERADRAGGLLTYGIPNMKLEKGIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDA
VILCTGAQKQRDLLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGGGDTGADCVATALRQKAKSVH
QFGKHPKLPPARTNDNMWPEQPHVFTLEYAYEEAEAKFGRDPREYSIQTTKMVADKNGKLKELHTIQMEKVKNEHGKYEF
RELPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVNSVNNKISAAYGDYQTNIDGVFAAGDARRGQSLIVWAINEGREVAR
EVDRYLMGSSVLP

Sequences:

>Translated_493_residues
MGKPTGFMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTPFCQIGADINGFTSGCPIYNLIPEWNDLV
YRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPKKRTGKKVAIVGS
GPAGLASADQLNQAGHSVTVFERADRAGGLLTYGIPNMKLEKGIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDA
VILCTGAQKQRDLLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGGGDTGADCVATALRQKAKSVH
QFGKHPKLPPARTNDNMWPEQPHVFTLEYAYEEAEAKFGRDPREYSIQTTKMVADKNGKLKELHTIQMEKVKNEHGKYEF
RELPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVNSVNNKISAAYGDYQTNIDGVFAAGDARRGQSLIVWAINEGREVAR
EVDRYLMGSSVLP
>Mature_492_residues
GKPTGFMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTPFCQIGADINGFTSGCPIYNLIPEWNDLVY
RGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPKKRTGKKVAIVGSG
PAGLASADQLNQAGHSVTVFERADRAGGLLTYGIPNMKLEKGIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDAV
ILCTGAQKQRDLLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGGGDTGADCVATALRQKAKSVHQ
FGKHPKLPPARTNDNMWPEQPHVFTLEYAYEEAEAKFGRDPREYSIQTTKMVADKNGKLKELHTIQMEKVKNEHGKYEFR
ELPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVNSVNNKISAAYGDYQTNIDGVFAAGDARRGQSLIVWAINEGREVARE
VDRYLMGSSVLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family

Homologues:

Organism=Homo sapiens, GI119943098, Length=486, Percent_Identity=24.2798353909465, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789606, Length=489, Percent_Identity=37.8323108384458, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=35.0730688935282, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI87082180, Length=427, Percent_Identity=34.6604215456674, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1788468, Length=467, Percent_Identity=25.6959314775161, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789243, Length=429, Percent_Identity=28.9044289044289, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17570289, Length=498, Percent_Identity=46.7871485943775, Blast_Score=466, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI71984108, Length=479, Percent_Identity=24.6346555323591, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=165, Percent_Identity=31.5151515151515, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=496, Percent_Identity=49.3951612903226, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=52.8571428571429, Blast_Score=530, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=52.8571428571429, Blast_Score=530, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=52.8571428571429, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=52.8571428571429, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24640763, Length=299, Percent_Identity=27.0903010033445, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI18858217, Length=299, Percent_Identity=27.0903010033445, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GLTB_BACSU (O34399)

Other databases:

- EMBL:   AL009126
- PIR:   H69634
- RefSeq:   NP_389726.2
- ProteinModelPortal:   O34399
- SMR:   O34399
- EnsemblBacteria:   EBBACT00000000114
- GeneID:   940053
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18440
- NMPDR:   fig|224308.1.peg.1848
- GenoList:   BSU18440
- GeneTree:   EBGT00050000000438
- HOGENOM:   HBG715033
- ProtClustDB:   PRK12810
- BioCyc:   BSUB:BSU18440-MONOMER
- BRENDA:   1.4.1.13
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- Gene3D:   G3DSA:1.10.1060.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01317

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; SSF46548 Helical_ferredxn

EC number: =1.4.1.13

Molecular weight: Translated: 54863; Mature: 54732

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTPFCQIGADI
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCHHHHCCCC
NGFTSGCPIYNLIPEWNDLVYRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISD
CCCCCCCCCEECCCCCHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVSIKNIERTIIDKGFENGWIQPRIPKKRTGKKVAIVGSGPAGLASADQLNQAGHSVTV
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEE
FERADRAGGLLTYGIPNMKLEKGIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECHHHHHHHCCE
VILCTGAQKQRDLLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEEC
GDTGADCVATALRQKAKSVHQFGKHPKLPPARTNDNMWPEQPHVFTLEYAYEEAEAKFGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCC
DPREYSIQTTKMVADKNGKLKELHTIQMEKVKNEHGKYEFRELPGTEKVWPAQLVFIAIG
CCHHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEHHCCCCCCCCCCEEEEEEEEC
FEGTEQPLLKQFGVNSVNNKISAAYGDYQTNIDGVFAAGDARRGQSLIVWAINEGREVAR
CCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHH
EVDRYLMGSSVLP
HHHHHHHCCCCCC
>Mature Secondary Structure 
GKPTGFMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTPFCQIGADI
CCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCHHHHCCCC
NGFTSGCPIYNLIPEWNDLVYRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISD
CCCCCCCCCEECCCCCHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVSIKNIERTIIDKGFENGWIQPRIPKKRTGKKVAIVGSGPAGLASADQLNQAGHSVTV
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEE
FERADRAGGLLTYGIPNMKLEKGIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECHHHHHHHCCE
VILCTGAQKQRDLLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEEC
GDTGADCVATALRQKAKSVHQFGKHPKLPPARTNDNMWPEQPHVFTLEYAYEEAEAKFGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCC
DPREYSIQTTKMVADKNGKLKELHTIQMEKVKNEHGKYEFRELPGTEKVWPAQLVFIAIG
CCHHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEHHCCCCCCCCCCEEEEEEEEC
FEGTEQPLLKQFGVNSVNNKISAAYGDYQTNIDGVFAAGDARRGQSLIVWAINEGREVAR
CCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHH
EVDRYLMGSSVLP
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411