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The map label for this gene is mlpA

Identifier: 255767376

GI number: 255767376

Start: 1742617

End: 1743846

Strand: Direct

Name: mlpA

Synonym: BSU16710

Alternate gene names: 255767376

Gene position: 1742617-1743846 (Clockwise)

Preceding gene: 16078733

Following gene: 16078735

Centisome position: 41.34

GC content: 43.01

Gene sequence:

>1230_bases
TTGATTAAACGATATACGTGTCAAAATGGTGTAAGAATTGTGCTGGAAAATAACCCGACAGTCCGTTCTGTTGCGATCGG
CGTATGGATCGGCACAGGTTCGAGACATGAAACGCCGGAGATAAACGGAATTTCTCACTTTTTAGAGCACATGTTCTTTA
AAGGGACGAGCACAAAATCTGCACGCGAGATAGCAGAATCTTTTGATCGTATTGGCGGCCAGGTCAATGCGTTTACTTCA
AAGGAATATACCTGCTACTATGCAAAGGTGCTTGACGAGCATGCGAATTACGCGCTGGACGTATTAGCAGATATGTTCTT
TCATTCAACGTTTGATGAAAACGAGCTGAAAAAAGAGAAAAATGTAGTATATGAAGAAATTAAAATGTATGAAGATGCGC
CGGACGACATTGTACATGATTTATTGAGCAAAGCCACTTACGGCAATCATTCTTTAGGCTACCCAATTTTAGGAACGGAA
GAAACGCTTGCTTCCTTTAACGGTGACTCTCTCAGACAATACATGCATGATTATTATACGCCGGACCGAGTGGTGATTTC
GGTAGCGGGCAATATATCTGACAGTTTTATCAAAGATGTAGAAAAATGGTTCGGGTCATACGAGGCGAAAGGCAAAGCGA
CAGGCCTTGAGAAGCCTGAGTTCCACACGGAAAAACTGACGAGAAAAAAAGAAACAGAGCAGGCTCATTTGTGCCTTGGA
TTTAAAGGCTTAGAGGTTGGCCATGAACGTATTTATGATTTAATCGTCCTCAACAATGTGCTCGGAGGCAGCATGAGCAG
CCGGCTGTTCCAAGATGTGCGTGAAGATAAAGGACTTGCTTATTCTGTTTACAGCTATCACAGCTCATATGAGGACAGCG
GAATGCTAACGATTTACGGCGGAACGGGTGCAAATCAGCTTCAGCAGCTGTCGGAAACAATTCAAGAAACCCTGGCCACA
TTAAAACGTGACGGCATTACCTCGAAAGAACTGGAAAACAGCAAAGAGCAAATGAAGGGAAGCTTAATGCTGAGCTTAGA
AAGCACAAACAGCAAAATGAGCCGAAACGGAAAAAATGAACTGCTGCTCGGCAAACATAAAACATTAGATGAAATTATCA
ATGAATTAAACGCCGTGAACTTAGAGCGTGTTAATGGCCTTGCCAGACAATTGTTTACCGAAGACTATGCATTAGCACTT
ATCAGCCCTTCCGGCAATATGCCGTCTTAA

Upstream 100 bases:

>100_bases
CAAAGGATATGCGCTTGGAACAGTCACTGAACTAATGGATGAAACAAGACTATTGAAGTAACAGATGAAGGCAGCTTGTC
TTTGAATAGGAGGAACAAAT

Downstream 100 bases:

>100_bases
AAAGGAAAGCCTGCCCCATAATGGAGCAGGCATTTTTTAATCCCTTTCATCATACATAGTACAAGGGGGTGACAGACATG
CGGCTCAGTGAATTATCGGG

Product: specific processing protease

Products: NA

Alternate protein names: ORFP

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MIKRYTCQNGVRIVLENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTSTKSAREIAESFDRIGGQVNAFTS
KEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEKNVVYEEIKMYEDAPDDIVHDLLSKATYGNHSLGYPILGTE
ETLASFNGDSLRQYMHDYYTPDRVVISVAGNISDSFIKDVEKWFGSYEAKGKATGLEKPEFHTEKLTRKKETEQAHLCLG
FKGLEVGHERIYDLIVLNNVLGGSMSSRLFQDVREDKGLAYSVYSYHSSYEDSGMLTIYGGTGANQLQQLSETIQETLAT
LKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHKTLDEIINELNAVNLERVNGLARQLFTEDYALAL
ISPSGNMPS

Sequences:

>Translated_409_residues
MIKRYTCQNGVRIVLENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTSTKSAREIAESFDRIGGQVNAFTS
KEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEKNVVYEEIKMYEDAPDDIVHDLLSKATYGNHSLGYPILGTE
ETLASFNGDSLRQYMHDYYTPDRVVISVAGNISDSFIKDVEKWFGSYEAKGKATGLEKPEFHTEKLTRKKETEQAHLCLG
FKGLEVGHERIYDLIVLNNVLGGSMSSRLFQDVREDKGLAYSVYSYHSSYEDSGMLTIYGGTGANQLQQLSETIQETLAT
LKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHKTLDEIINELNAVNLERVNGLARQLFTEDYALAL
ISPSGNMPS
>Mature_409_residues
MIKRYTCQNGVRIVLENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTSTKSAREIAESFDRIGGQVNAFTS
KEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEKNVVYEEIKMYEDAPDDIVHDLLSKATYGNHSLGYPILGTE
ETLASFNGDSLRQYMHDYYTPDRVVISVAGNISDSFIKDVEKWFGSYEAKGKATGLEKPEFHTEKLTRKKETEQAHLCLG
FKGLEVGHERIYDLIVLNNVLGGSMSSRLFQDVREDKGLAYSVYSYHSSYEDSGMLTIYGGTGANQLQQLSETIQETLAT
LKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHKTLDEIINELNAVNLERVNGLARQLFTEDYALAL
ISPSGNMPS

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family

Homologues:

Organism=Homo sapiens, GI94538354, Length=420, Percent_Identity=27.8571428571429, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI24308013, Length=439, Percent_Identity=25.5125284738041, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI46593007, Length=415, Percent_Identity=22.4096385542169, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI155969707, Length=233, Percent_Identity=30.9012875536481, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI50592988, Length=417, Percent_Identity=19.9040767386091, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1787770, Length=187, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71999683, Length=401, Percent_Identity=25.4364089775561, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17553678, Length=419, Percent_Identity=24.8210023866348, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17510601, Length=364, Percent_Identity=22.2527472527473, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566665, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25146563, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71994338, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25146566, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323192, Length=389, Percent_Identity=27.2493573264781, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321813, Length=419, Percent_Identity=25.2983293556086, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319426, Length=413, Percent_Identity=23.2445520581114, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357875, Length=415, Percent_Identity=27.710843373494, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24646943, Length=415, Percent_Identity=27.710843373494, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI19921772, Length=442, Percent_Identity=24.2081447963801, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YMXG_BACSU (Q04805)

Other databases:

- EMBL:   U27560
- EMBL:   AL009126
- EMBL:   L08471
- PIR:   E69886
- RefSeq:   NP_389553.2
- ProteinModelPortal:   Q04805
- SMR:   Q04805
- MEROPS:   M16.016
- EnsemblBacteria:   EBBACT00000000542
- GeneID:   938456
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16710
- NMPDR:   fig|224308.1.peg.1674
- GenoList:   BSU16710
- GeneTree:   EBGT00050000001968
- HOGENOM:   HBG476859
- ProtClustDB:   CLSK873315
- BioCyc:   BSUB:BSU16710-MONOMER
- GO:   GO:0006508
- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863
- Gene3D:   G3DSA:3.30.830.10

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C; SSF63411 Metalloenz_metal-bd

EC number: 3.4.99.- [C]

Molecular weight: Translated: 45995; Mature: 45995

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00143 INSULINASE

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYTCQNGVRIVLENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTSTKS
CCCCEECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
AREIAESFDRIGGQVNAFTSKEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEK
HHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NVVYEEIKMYEDAPDDIVHDLLSKATYGNHSLGYPILGTEETLASFNGDSLRQYMHDYYT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCC
PDRVVISVAGNISDSFIKDVEKWFGSYEAKGKATGLEKPEFHTEKLTRKKETEQAHLCLG
CCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEEEC
FKGLEVGHERIYDLIVLNNVLGGSMSSRLFQDVREDKGLAYSVYSYHSSYEDSGMLTIYG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEEC
GTGANQLQQLSETIQETLATLKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNE
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCC
LLLGKHKTLDEIINELNAVNLERVNGLARQLFTEDYALALISPSGNMPS
EEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MIKRYTCQNGVRIVLENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTSTKS
CCCCEECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
AREIAESFDRIGGQVNAFTSKEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEK
HHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NVVYEEIKMYEDAPDDIVHDLLSKATYGNHSLGYPILGTEETLASFNGDSLRQYMHDYYT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCC
PDRVVISVAGNISDSFIKDVEKWFGSYEAKGKATGLEKPEFHTEKLTRKKETEQAHLCLG
CCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEEEC
FKGLEVGHERIYDLIVLNNVLGGSMSSRLFQDVREDKGLAYSVYSYHSSYEDSGMLTIYG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEEC
GTGANQLQQLSETIQETLATLKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNE
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCC
LLLGKHKTLDEIINELNAVNLERVNGLARQLFTEDYALALISPSGNMPS
EEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 8098035