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The map label for this gene is carA

Identifier: 255767254

GI number: 255767254

Start: 1199327

End: 1200388

Strand: Direct

Name: carA

Synonym: BSU11230

Alternate gene names: 255767254

Gene position: 1199327-1200388 (Clockwise)

Preceding gene: 255767253

Following gene: 255767255

Centisome position: 28.45

GC content: 46.89

Gene sequence:

>1062_bases
ATGGAAGGTTATTTAGTGTTAGAAGATGGGACATCGTTCAGCGGCGAGCTGGACGGTCATGAAAACTGCACGGGAGAAGC
TGTTTTTTTCACAGGAATGACAGGCTACCAGGAAGTGCTGACAGATCCATCATATAAGGGACAGATTATTGTATTTACCT
ACCCGCTGATTGGCAACTACGGCATAAATGAAAAGGATTTTGAAAGCAAGAAACCGCAAGTAAAAGCCGCGGTTGTCTAT
GAGGCGTGTGATCACTTTTCTCATTATGAAGCGGTATACAGCCTCAAGGAGTATCTACAGAAATGGAATATTCCGCTGTT
AACCCATGTTGATACGAGGGCTGTCGTGAAGAAAATCCGGGCAAACGGGACGATGGGTGCGACAGTCACAGCCTCTAAAG
AAGGAGCTGAGATTGCCCTTCAACCAGAGAATGTGGCAGAGCAGGCTTCCGCCCAGGAAATCAGCACATTCGGCGACGGG
AATAAACATATCGCTCTCATTGATTTTGGCTATAAAAAGTCAATCGCATCATCACTTGTCAAACGAGGCTGCAAGGTTAC
CGTGGTGCCATACCAGCAAATGGAGGCCGTATACAACATCAAACCCGATGGAATTGTGCTATCGAACGGACCTGGAGATC
CGAAAGCCATTCAGCCATATTTAGGAAAGATCAAAAGCATCATCAGCCGCTTTCCGACTCTAGGCATTTGTCTCGGACAT
CAGCTGATCGCGCTCGCGTTTGGAGGGAATACTTTTAAGCTGCCATTCGGGCACAGGGGAGCTAATCATCCGGTCATCGA
CCGTAAGACGAAACGGGTCTTTATGACAAGCCAAAACCACAGCTATGTGGTTGATGAACAGTCGATTAATGAAGAAGAGC
TCACCATCAGGTTTCATCATGTCAATGATACGTCAGTTGAAGGGCTTGCCCATAAAAAACTGCCTGTCATGAGTGTTCAA
TTCCACCCGGAAGCCCATCCCGGACCTGCGGAAAGCGAATGGATTTTTGATGATTATCTAAAGAATGTGATACCAGCAAG
GAGAGAAATCGCGCATGCCTAA

Upstream 100 bases:

>100_bases
ATCGCTCATCATTCCGCTGTAAACCAGTGATTTTTTTTCGATATAAAGGCATAAAAATTCATTTTTATAATTAATTATTC
ATCAAGAGGTGGGCTGTTAG

Downstream 100 bases:

>100_bases
AGACACCAGTATTTCAAGCATATTAGTAATTGGCTCAGGCCCGATCATCATCGGGCAGGCGGCAGAATTTGATTATTCAG
GCACTCAAGGGTGCATCGCG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINEKDFESKKPQVKAAVVY
EACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIRANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDG
NKHIALIDFGYKKSIASSLVKRGCKVTVVPYQQMEAVYNIKPDGIVLSNGPGDPKAIQPYLGKIKSIISRFPTLGICLGH
QLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHHVNDTSVEGLAHKKLPVMSVQ
FHPEAHPGPAESEWIFDDYLKNVIPARREIAHA

Sequences:

>Translated_353_residues
MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINEKDFESKKPQVKAAVVY
EACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIRANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDG
NKHIALIDFGYKKSIASSLVKRGCKVTVVPYQQMEAVYNIKPDGIVLSNGPGDPKAIQPYLGKIKSIISRFPTLGICLGH
QLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHHVNDTSVEGLAHKKLPVMSVQ
FHPEAHPGPAESEWIFDDYLKNVIPARREIAHA
>Mature_353_residues
MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINEKDFESKKPQVKAAVVY
EACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIRANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDG
NKHIALIDFGYKKSIASSLVKRGCKVTVVPYQQMEAVYNIKPDGIVLSNGPGDPKAIQPYLGKIKSIISRFPTLGICLGH
QLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHHVNDTSVEGLAHKKLPVMSVQ
FHPEAHPGPAESEWIFDDYLKNVIPARREIAHA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=357, Percent_Identity=42.0168067226891, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI169790915, Length=354, Percent_Identity=33.8983050847458, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI21361331, Length=354, Percent_Identity=33.8983050847458, Blast_Score=214, Evalue=7e-56,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=36.6310160427808, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1789760, Length=156, Percent_Identity=29.4871794871795, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=369, Percent_Identity=39.8373983739837, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324878, Length=361, Percent_Identity=37.9501385041551, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=390, Percent_Identity=36.4102564102564, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=37.8666666666667, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45555749, Length=373, Percent_Identity=38.0697050938338, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARX_BACSU (P36838)

Other databases:

- EMBL:   Z26919
- EMBL:   AL009126
- PIR:   I40376
- RefSeq:   NP_389005.2
- ProteinModelPortal:   P36838
- SMR:   P36838
- EnsemblBacteria:   EBBACT00000001321
- GeneID:   936387
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11230
- NMPDR:   fig|224308.1.peg.1124
- GenoList:   BSU11230
- GeneTree:   EBGT00070000032080
- HOGENOM:   HBG286341
- ProtClustDB:   PRK12838
- BioCyc:   BSUB:BSU11230-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39084; Mature: 39084

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 237-237 ACT_SITE 322-322 ACT_SITE 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNY
CCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCC
GINEKDFESKKPQVKAAVVYEACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
ANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDGNKHIALIDFGYKKSIASSLV
CCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHH
KRGCKVTVVPYQQMEAVYNIKPDGIVLSNGPGDPKAIQPYLGKIKSIISRFPTLGICLGH
HCCCEEEEECHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHC
QLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHH
EEEEEEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEEE
VNDTSVEGLAHKKLPVMSVQFHPEAHPGPAESEWIFDDYLKNVIPARREIAHA
CCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNY
CCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCC
GINEKDFESKKPQVKAAVVYEACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
ANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDGNKHIALIDFGYKKSIASSLV
CCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHH
KRGCKVTVVPYQQMEAVYNIKPDGIVLSNGPGDPKAIQPYLGKIKSIISRFPTLGICLGH
HCCCEEEEECHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHC
QLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHH
EEEEEEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEEE
VNDTSVEGLAHKKLPVMSVQFHPEAHPGPAESEWIFDDYLKNVIPARREIAHA
CCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8025667; 9384377