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The map label for this gene is cspR

Identifier: 255767208

GI number: 255767208

Start: 970135

End: 970617

Strand: Direct

Name: cspR

Synonym: BSU08930

Alternate gene names: 255767208

Gene position: 970135-970617 (Clockwise)

Preceding gene: 16077957

Following gene: 16077959

Centisome position: 23.01

GC content: 40.99

Gene sequence:

>483_bases
GTGAACATTGTGGCATTACATGTCGTTTTATATCAACCAGAAATTCCCGCTAACACAGGGAATATTGCACGTACTTGTGC
AGCAACCAATACAACGCTACACCTGATTCGTCCGCTCGGCTTTTCAACGGATGATAAAATGCTGAAGCGTGCCGGACTCG
ATTATTGGGAGTTTGTTAACGTTGTGTATCATGATTCGTTAGAAGAATTGTTTGAGGCATATAAAAAAGGCAAGTTTTTC
TTTATTACAAAATTTGGCCAGCAGCCTCATACATCATTTGATTACACTGATCTTGACGAGGATTATTTCTTCGTGTTCGG
AAGAGAAACAAGCGGTTTGCCAAAGGATTTAATTCAAAATAATATGGACCGCTGCCTGCGTCTTCCGATGACAGAGCATG
TCAGATCGTTAAATCTGTCCAATACTGCCGCGATTCTTGTTTATGAAGCGCTGCGCCAGCAAAATTACCGTGATTTAAAA
TAG

Upstream 100 bases:

>100_bases
GCATACACGCCGTCAATACGATATGCCTTTTTTCATATCGTTGATGATACGACAAAAGAGTAATTAATGTTATAATGATG
GGCGGATAAGAAAAACAGAG

Downstream 100 bases:

>100_bases
TGAAAAACCCGCTCATCGATGATGGGCGGGTTTTTTTGCGGATGTTGAAATGCTTTGTTTCGTTTTTGGGCTTAACAACG
TAACAGTGTTATGTTACAAT

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFF
FITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK

Sequences:

>Translated_160_residues
MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFF
FITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK
>Mature_160_residues
MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFF
FITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=45.3947368421053, Blast_Score=138, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRML_BACSU (O31590)

Other databases:

- EMBL:   U58864
- EMBL:   AL009126
- RefSeq:   NP_388774.2
- HSSP:   P44868
- ProteinModelPortal:   O31590
- SMR:   O31590
- EnsemblBacteria:   EBBACT00000003300
- GeneID:   939735
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08930
- GenoList:   BSU08930
- GeneTree:   EBGT00050000002343
- HOGENOM:   HBG309728
- ProtClustDB:   CLSK886924
- BioCyc:   BSUB:BSU08930-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01885
- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537
- PIRSF:   PIRSF029256
- TIGRFAMs:   TIGR00185

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18577; Mature: 18577

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 107-107 BINDING 128-128 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVN
CCEEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHH
VVYHDSLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQN
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHC
NMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK
CHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVN
CCEEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHH
VVYHDSLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQN
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHC
NMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK
CHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377