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The map label for this gene is catE

Identifier: 255767192

GI number: 255767192

Start: 898010

End: 898867

Strand: Direct

Name: catE

Synonym: BSU08240

Alternate gene names: 255767192

Gene position: 898010-898867 (Clockwise)

Preceding gene: 16077890

Following gene: 16077892

Centisome position: 21.3

GC content: 50.23

Gene sequence:

>858_bases
ATGACCAGCATTCATGAGGATACACACATTGGCTATGCCAAACTCACAATTCGCAGTCTGGAGCGTTCGCTTCAGTTCTA
CTGTAACGTCATCGGTTTTCAAGTCTTAAAAAAGACGGACCGCCAAGCGGAATTAACGGCTGACGGGAAACGCGTACTGC
TTATTCTTGAAGAAAATCCGAGCGCTGTCGTCTTGCCTGAACGGTCTGTGACAGGCCTTTATCACTTTGCGATTCTTCTT
CCGGACAGGAAAGAACTTGGGATCGCGCTCGCCCGCCTGATTGAACACGGCATTGCTATCGGACATGGAGACCATGCTGT
CAGCGAAGCGCTCTACCTGTCTGATCCTGACGGAAACGGCATTGAAATGTACGCTGACCGCCCCCGCAGCACGTGGCAGC
GTGATCGGGAAGGAAACTACGTCATGACGACAACCGCTGTTGACATTGAGGGCCTTCTGGAAGAGGCAGGAGATGAGCGG
AAAACATCGCTTCCGAACGATACAATCATCGGACACATTCATCTGCATGTCAGTGATTTGAAAGAAGCAAAGGCGTTTTA
TACAGATGTACTGGGCTTTGACATTGTCGGAAACTATGCGGGCATGTCCGCCCTCTTCGTTTCAGCCGGGGGGTACCACC
ATCATATCGGCTTAAACATTTGGGCGGGAAGAAATGCGCCGCCTAAACCGACAAATGCCAGCGGACTAGATTATTATACT
GTTGTCCTGCCCCACCAAGAGGAGCTGGATTTGGTGGCAAACCGAGTCAAACATGCCGGGTATTCGATTGAAGAGACGGA
AAACAGCTTTCGTGTGAAAGACCCTGTTTCCGGCGCCTATATTACATTTGTGATTTAA

Upstream 100 bases:

>100_bases
ACCTGGCGCTTACTGGAAGTCGATTCCTGGCGCTGGACCCATTTGTGTTTAAAGGAAAAAAGAACGGAAACGTTTCAGCA
TAAGAAAAGAGGCATGCAGC

Downstream 100 bases:

>100_bases
CTGCAATCCCCTTGCCGAAATAACGGCAGGGGGATTTTTTATTTTTGTCCGCCTTGTTATGATGAGAGAACCAACTTGTT
AGGAGGCGTTTACATGGAAC

Product: catechol-2,3-dioxygenase subunit

Products: NA

Alternate protein names: Catechol-induced ring cleavage extradiol dioxygenase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTSIHEDTHIGYAKLTIRSLERSLQFYCNVIGFQVLKKTDRQAELTADGKRVLLILEENPSAVVLPERSVTGLYHFAILL
PDRKELGIALARLIEHGIAIGHGDHAVSEALYLSDPDGNGIEMYADRPRSTWQRDREGNYVMTTTAVDIEGLLEEAGDER
KTSLPNDTIIGHIHLHVSDLKEAKAFYTDVLGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGRNAPPKPTNASGLDYYT
VVLPHQEELDLVANRVKHAGYSIEETENSFRVKDPVSGAYITFVI

Sequences:

>Translated_285_residues
MTSIHEDTHIGYAKLTIRSLERSLQFYCNVIGFQVLKKTDRQAELTADGKRVLLILEENPSAVVLPERSVTGLYHFAILL
PDRKELGIALARLIEHGIAIGHGDHAVSEALYLSDPDGNGIEMYADRPRSTWQRDREGNYVMTTTAVDIEGLLEEAGDER
KTSLPNDTIIGHIHLHVSDLKEAKAFYTDVLGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGRNAPPKPTNASGLDYYT
VVLPHQEELDLVANRVKHAGYSIEETENSFRVKDPVSGAYITFVI
>Mature_284_residues
TSIHEDTHIGYAKLTIRSLERSLQFYCNVIGFQVLKKTDRQAELTADGKRVLLILEENPSAVVLPERSVTGLYHFAILLP
DRKELGIALARLIEHGIAIGHGDHAVSEALYLSDPDGNGIEMYADRPRSTWQRDREGNYVMTTTAVDIEGLLEEAGDERK
TSLPNDTIIGHIHLHVSDLKEAKAFYTDVLGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGRNAPPKPTNASGLDYYTV
VLPHQEELDLVANRVKHAGYSIEETENSFRVKDPVSGAYITFVI

Specific function: Involved in the meta cleavage of catechol to 2- hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol

COG id: COG2514

COG function: function code R; Predicted ring-cleavage extradiol dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CADE_BACSU (P54721)

Other databases:

- EMBL:   D50543
- EMBL:   AL009126
- PIR:   H69802
- RefSeq:   NP_388705.2
- ProteinModelPortal:   P54721
- EnsemblBacteria:   EBBACT00000000801
- GeneID:   936164
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08240
- NMPDR:   fig|224308.1.peg.824
- GenoList:   BSU08240
- GeneTree:   EBGT00050000001446
- HOGENOM:   HBG704761
- ProtClustDB:   CLSK886889
- BioCyc:   BSUB:BSU08240-MONOMER
- InterPro:   IPR004360
- InterPro:   IPR000486

Pfam domain/function: PF00903 Glyoxalase

EC number: =1.13.11.2

Molecular weight: Translated: 31565; Mature: 31434

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIHEDTHIGYAKLTIRSLERSLQFYCNVIGFQVLKKTDRQAELTADGKRVLLILEENP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCC
SAVVLPERSVTGLYHFAILLPDRKELGIALARLIEHGIAIGHGDHAVSEALYLSDPDGNG
CEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCEEECCCCHHHHCEEEEECCCCCC
IEMYADRPRSTWQRDREGNYVMTTTAVDIEGLLEEAGDERKTSLPNDTIIGHIHLHVSDL
EEEEECCCCHHHHHCCCCCEEEEEEEECHHHHHHHCCCCCCCCCCCCEEEEEEEEEEHHH
KEAKAFYTDVLGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGRNAPPKPTNASGLDYYT
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCEEEE
VVLPHQEELDLVANRVKHAGYSIEETENSFRVKDPVSGAYITFVI
EEECCHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCEEEEEEC
>Mature Secondary Structure 
TSIHEDTHIGYAKLTIRSLERSLQFYCNVIGFQVLKKTDRQAELTADGKRVLLILEENP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCC
SAVVLPERSVTGLYHFAILLPDRKELGIALARLIEHGIAIGHGDHAVSEALYLSDPDGNG
CEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCEEECCCCHHHHCEEEEECCCCCC
IEMYADRPRSTWQRDREGNYVMTTTAVDIEGLLEEAGDERKTSLPNDTIIGHIHLHVSDL
EEEEECCCCHHHHHCCCCCEEEEEEEECHHHHHHHCCCCCCCCCCCCEEEEEEEEEEHHH
KEAKAFYTDVLGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGRNAPPKPTNASGLDYYT
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCEEEE
VVLPHQEELDLVANRVKHAGYSIEETENSFRVKDPVSGAYITFVI
EEECCHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8704981; 9384377