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The map label for this gene is yfiF

Identifier: 16077892

GI number: 16077892

Start: 898961

End: 899905

Strand: Direct

Name: yfiF

Synonym: BSU08250

Alternate gene names: 16077892

Gene position: 898961-899905 (Clockwise)

Preceding gene: 255767192

Following gene: 16077893

Centisome position: 21.32

GC content: 43.28

Gene sequence:

>945_bases
ATGGAACTCAACCACCTTGTGACGGGCAAAATAGCATTAAATCAATATGTGCATCGCTTAGAGCAAAAGGATGTTTCATT
TTACGTCCACTACTGGGGAGCGATGACGAACCACTATAACACACTGCTTCATAAGCATTCCTTTTATGAAATTTGTTACG
TGGTAAGAGGTGAAGGATATTACCTTGAAGGCGATCGTACATATCCGCTTCGTGAACAGACGATTTTTTTGTCAAAGCCG
GAGCATTTGCACCAAATCAAAAGTGAAACAGGGCTGTTCCTTTTTTACGTCGCTTTCGAGCTGAGCGAGGATCAGTCAAG
CGCCGAATGGATCAAAGTGATGGAAGAAGTGAAACAAAGCCCCTTTATCACAATGCAATTGAAAGATGGCGAACCGCCGG
GCCTGCTTTGGAAAACGTTAATGCTGCAAGCAGCGCAACCTGTCAATGCTTTTGACAAAGAGATCCTATCTCATTTGTCG
CATGCATTGATCCTGTCATTAATCCAAACCTTTCTCCCGTACGCCCAACGCCCGAAACAAAAGCAGCCCATTCACACATC
TTCTGCTTTGCTGACAGAGGTGAAACTGCACATAAAAGACAATCTGTCACAGCCGTTAAAGCTGACGGATGTCGCTAGCC
ACTTTCATATCTCAGGGAGGCATTTATCACGTTTATTTGCAGCAGAACTGGGCGTCAGCTACTCAGAATTTGTCCAAAAT
GAAAAAATAAACAAAGCTGCCGAACTGCTGAAATCAACGAATTTGTCGATTAAAGAAATAGCGGAAGAAATCGGTTTCTC
AGTACATTATTTCACTCGCGTCTTTTCCGCTAAAATCGGCAGCTCTCCAGGGCTTTTCCGCTCCCTTTATAAAGACTCAA
AAATGACCGCATTTCAAATCAATAAACCTTTTCAAAAAATCGAACAGGCCAAGCCGCACGAATAA

Upstream 100 bases:

>100_bases
GATTTAACTGCAATCCCCTTGCCGAAATAACGGCAGGGGGATTTTTTATTTTTGTCCGCCTTGTTATGATGAGAGAACCA
ACTTGTTAGGAGGCGTTTAC

Downstream 100 bases:

>100_bases
GATGTCCTGAAATGACAAAACAATGTCTTCATATGATAAAGACGCGAGAAATCTCGTTGTCTTATAATCTTTTTAGAAAG
CGGTTACAACAAGGGGTATT

Product: AraC/XylS family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MELNHLVTGKIALNQYVHRLEQKDVSFYVHYWGAMTNHYNTLLHKHSFYEICYVVRGEGYYLEGDRTYPLREQTIFLSKP
EHLHQIKSETGLFLFYVAFELSEDQSSAEWIKVMEEVKQSPFITMQLKDGEPPGLLWKTLMLQAAQPVNAFDKEILSHLS
HALILSLIQTFLPYAQRPKQKQPIHTSSALLTEVKLHIKDNLSQPLKLTDVASHFHISGRHLSRLFAAELGVSYSEFVQN
EKINKAAELLKSTNLSIKEIAEEIGFSVHYFTRVFSAKIGSSPGLFRSLYKDSKMTAFQINKPFQKIEQAKPHE

Sequences:

>Translated_314_residues
MELNHLVTGKIALNQYVHRLEQKDVSFYVHYWGAMTNHYNTLLHKHSFYEICYVVRGEGYYLEGDRTYPLREQTIFLSKP
EHLHQIKSETGLFLFYVAFELSEDQSSAEWIKVMEEVKQSPFITMQLKDGEPPGLLWKTLMLQAAQPVNAFDKEILSHLS
HALILSLIQTFLPYAQRPKQKQPIHTSSALLTEVKLHIKDNLSQPLKLTDVASHFHISGRHLSRLFAAELGVSYSEFVQN
EKINKAAELLKSTNLSIKEIAEEIGFSVHYFTRVFSAKIGSSPGLFRSLYKDSKMTAFQINKPFQKIEQAKPHE
>Mature_314_residues
MELNHLVTGKIALNQYVHRLEQKDVSFYVHYWGAMTNHYNTLLHKHSFYEICYVVRGEGYYLEGDRTYPLREQTIFLSKP
EHLHQIKSETGLFLFYVAFELSEDQSSAEWIKVMEEVKQSPFITMQLKDGEPPGLLWKTLMLQAAQPVNAFDKEILSHLS
HALILSLIQTFLPYAQRPKQKQPIHTSSALLTEVKLHIKDNLSQPLKLTDVASHFHISGRHLSRLFAAELGVSYSEFVQN
EKINKAAELLKSTNLSIKEIAEEIGFSVHYFTRVFSAKIGSSPGLFRSLYKDSKMTAFQINKPFQKIEQAKPHE

Specific function: Activator Of The L-Rhamnose Operon. Rhar Putatively Autoregulates Its Synthesis. [C]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI171474011, Length=252, Percent_Identity=23.4126984126984, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788725, Length=95, Percent_Identity=36.8421052631579, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFIF_BACSU (P54722)

Other databases:

- EMBL:   D50543
- EMBL:   AL009126
- PIR:   A69803
- RefSeq:   NP_388706.1
- ProteinModelPortal:   P54722
- EnsemblBacteria:   EBBACT00000000007
- GeneID:   939704
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08250
- NMPDR:   fig|224308.1.peg.825
- GenoList:   BSU08250
- GeneTree:   EBGT00050000000043
- OMA:   HEICLAY
- PhylomeDB:   P54722
- ProtClustDB:   CLSK2518209
- BioCyc:   BSUB:BSU08250-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- SMART:   SM00342

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC; SSF51215 AraC_binding; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 36197; Mature: 36197

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNHLVTGKIALNQYVHRLEQKDVSFYVHYWGAMTNHYNTLLHKHSFYEICYVVRGEGY
CCCCCEEEHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCCE
YLEGDRTYPLREQTIFLSKPEHLHQIKSETGLFLFYVAFELSEDQSSAEWIKVMEEVKQS
EECCCCCCCCCCCEEEECCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHC
PFITMQLKDGEPPGLLWKTLMLQAAQPVNAFDKEILSHLSHALILSLIQTFLPYAQRPKQ
CEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KQPIHTSSALLTEVKLHIKDNLSQPLKLTDVASHFHISGRHLSRLFAAELGVSYSEFVQN
CCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
EKINKAAELLKSTNLSIKEIAEEIGFSVHYFTRVFSAKIGSSPGLFRSLYKDSKMTAFQI
CHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEC
NKPFQKIEQAKPHE
CCHHHHHHHCCCCC
>Mature Secondary Structure
MELNHLVTGKIALNQYVHRLEQKDVSFYVHYWGAMTNHYNTLLHKHSFYEICYVVRGEGY
CCCCCEEEHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCCE
YLEGDRTYPLREQTIFLSKPEHLHQIKSETGLFLFYVAFELSEDQSSAEWIKVMEEVKQS
EECCCCCCCCCCCEEEECCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHC
PFITMQLKDGEPPGLLWKTLMLQAAQPVNAFDKEILSHLSHALILSLIQTFLPYAQRPKQ
CEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KQPIHTSSALLTEVKLHIKDNLSQPLKLTDVASHFHISGRHLSRLFAAELGVSYSEFVQN
CCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
EKINKAAELLKSTNLSIKEIAEEIGFSVHYFTRVFSAKIGSSPGLFRSLYKDSKMTAFQI
CHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEC
NKPFQKIEQAKPHE
CCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8704981; 9384377