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The map label for this gene is glnT

Identifier: 255767059

GI number: 255767059

Start: 262732

End: 264168

Strand: Reverse

Name: glnT

Synonym: BSU02420

Alternate gene names: 255767059

Gene position: 264168-262732 (Counterclockwise)

Preceding gene: 16077312

Following gene: 16077310

Centisome position: 6.27

GC content: 46.83

Gene sequence:

>1437_bases
ATGCAGCAAATCCTAGAACATATCGTCGGTATTGCAAATGATCTTTTGTGGTCAAAGCTATTAATTGTTTTGCTCCTTTC
TTTTGGAATCTATTTTACGTTCAGGCTTAAGTTTTTACAGGTTCGCATGCTGAAAGAAATGGTCAGGGTATTAAGAGAAG
GTGCTGCTTCCCGGTCAAAAAACAGCATTTCTCCGTTTCAAGCCTTTTGCATCAGCATGGCGGCCCGTGTCGGAACGGGA
AATATTACGGGCATTGCTATCGCCATTGCACTCGGCGGGCCCGGTGCAATCTTCTGGATGTGGATTATCGCCATTATCGG
ATCAGCATCCAGCTTTGTGGAAAGCACACTGGCTCAGATTTATAAAGTAAAAGATGTAAACGGCTTCCGCGGCGGGCCGG
CGTATTACATGGAAAAAGGGCTGAACAAAAGGTGGATGGGCGCGCTGTTCGCTGTGTTAATCACCTTATCGTTCGGTATC
GTCTTCAACTCGGTTCAATCCAATACAGTCAGCCTTGCCTTTGAAAACGCGTTTGGCACAAACCGCCTGACATTGGGACT
CATTTTAATTGCTGTATTCGGCACGATTATTTTTGGCGGAGTCAAGCGCATTGCAAAGCTGGCTGAATCCATTGTCGTCG
TATTGGCTGTCCTGTATATCGGAGTTGCGTTTTTCGTCATTTTCTCCAACATTACGCAGCTGCCAGGTGTGCTTGCGCTA
ATCGTGAAAAATGCGTTTGGTTTTGACCAGGCCGCCGGCGGGGCTTTGGGCGCGGCACTCATGCAAGGCGTCAGACGGGG
AATCTTTTCAAACGAAGCGGGAATGGGAAGCGCACCAAATGCCGCTGCCACCGCCACAACAAGCCATCCCGTCAAACAGG
GGCTGATCCAAGCTTTTGGTGTACTGACAGATACACTGGTCATTTGTACAAGCACCGCTTTTATCATTCTGTTTTCGGAT
GCCTATCATACTCCGGGTCTGAGCGGAATCGCCTTGACACAAGCCTCCCTCAGCTCCCATGTCGGATCATGGGCATCAGG
CTTTCTCGCGATTCTGATTTTGTTATTCGGATTCTGTGCTCTAATTGGAAACTATTATTATGGCGAAACCAATATCGGCT
TTTTGAACAAAAGCAAAAAACTGATTTTTGTCTACCGTATCGGCGTGCTGGCAATGATCGTGTTTGGCTGTGTTGCCAAA
GTCCAGCTCGTCTGGGATCTTGCTGATTTGTTCATGGGTTTGATGGTCATCGTGAATTTAATCGCGATTTTCTTACTATC
AAAGGTCGTTTTTACGGCGCTGAAAGACTATACACGCCAGAAAAAAGCAGGCAAGGATCCGGTCTTTTATAAAGATGTGC
TCAAAAACCATAATGGCATTGAGTGCTGGCCGGTTTCAGACACAAAGACTGACACGCACAACAAACAAATATCATAA

Upstream 100 bases:

>100_bases
GATGAATACGGCAACAGCCTCACTGGCGGTATGCTGCTGAAGCATATGGCGCAGGAATGGGAGCTCAGCATTTTTTAAAC
ATGAAAAAAGAGGTGATTCT

Downstream 100 bases:

>100_bases
GAAAAGGGTGTTCAGACATATGGGCTGAACACCTTTCTCTTTTTGCGTTTATTGACAAACACGTAAGCCTCCATTACGAT
TTTCATATCTTTTTCAAAAA

Product: glutamine transporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MQQILEHIVGIANDLLWSKLLIVLLLSFGIYFTFRLKFLQVRMLKEMVRVLREGAASRSKNSISPFQAFCISMAARVGTG
NITGIAIAIALGGPGAIFWMWIIAIIGSASSFVESTLAQIYKVKDVNGFRGGPAYYMEKGLNKRWMGALFAVLITLSFGI
VFNSVQSNTVSLAFENAFGTNRLTLGLILIAVFGTIIFGGVKRIAKLAESIVVVLAVLYIGVAFFVIFSNITQLPGVLAL
IVKNAFGFDQAAGGALGAALMQGVRRGIFSNEAGMGSAPNAAATATTSHPVKQGLIQAFGVLTDTLVICTSTAFIILFSD
AYHTPGLSGIALTQASLSSHVGSWASGFLAILILLFGFCALIGNYYYGETNIGFLNKSKKLIFVYRIGVLAMIVFGCVAK
VQLVWDLADLFMGLMVIVNLIAIFLLSKVVFTALKDYTRQKKAGKDPVFYKDVLKNHNGIECWPVSDTKTDTHNKQIS

Sequences:

>Translated_478_residues
MQQILEHIVGIANDLLWSKLLIVLLLSFGIYFTFRLKFLQVRMLKEMVRVLREGAASRSKNSISPFQAFCISMAARVGTG
NITGIAIAIALGGPGAIFWMWIIAIIGSASSFVESTLAQIYKVKDVNGFRGGPAYYMEKGLNKRWMGALFAVLITLSFGI
VFNSVQSNTVSLAFENAFGTNRLTLGLILIAVFGTIIFGGVKRIAKLAESIVVVLAVLYIGVAFFVIFSNITQLPGVLAL
IVKNAFGFDQAAGGALGAALMQGVRRGIFSNEAGMGSAPNAAATATTSHPVKQGLIQAFGVLTDTLVICTSTAFIILFSD
AYHTPGLSGIALTQASLSSHVGSWASGFLAILILLFGFCALIGNYYYGETNIGFLNKSKKLIFVYRIGVLAMIVFGCVAK
VQLVWDLADLFMGLMVIVNLIAIFLLSKVVFTALKDYTRQKKAGKDPVFYKDVLKNHNGIECWPVSDTKTDTHNKQIS
>Mature_478_residues
MQQILEHIVGIANDLLWSKLLIVLLLSFGIYFTFRLKFLQVRMLKEMVRVLREGAASRSKNSISPFQAFCISMAARVGTG
NITGIAIAIALGGPGAIFWMWIIAIIGSASSFVESTLAQIYKVKDVNGFRGGPAYYMEKGLNKRWMGALFAVLITLSFGI
VFNSVQSNTVSLAFENAFGTNRLTLGLILIAVFGTIIFGGVKRIAKLAESIVVVLAVLYIGVAFFVIFSNITQLPGVLAL
IVKNAFGFDQAAGGALGAALMQGVRRGIFSNEAGMGSAPNAAATATTSHPVKQGLIQAFGVLTDTLVICTSTAFIILFSD
AYHTPGLSGIALTQASLSSHVGSWASGFLAILILLFGFCALIGNYYYGETNIGFLNKSKKLIFVYRIGVLAMIVFGCVAK
VQLVWDLADLFMGLMVIVNLIAIFLLSKVVFTALKDYTRQKKAGKDPVFYKDVLKNHNGIECWPVSDTKTDTHNKQIS

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family

Homologues:

Organism=Escherichia coli, GI1786188, Length=463, Percent_Identity=43.8444924406048, Blast_Score=347, Evalue=9e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLNT_BACSU (O31464)

Other databases:

- EMBL:   AB006424
- EMBL:   AL009126
- PIR:   C69751
- RefSeq:   NP_388124.2
- ProteinModelPortal:   O31464
- EnsemblBacteria:   EBBACT00000001738
- GeneID:   938419
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02420
- NMPDR:   fig|224308.1.peg.243
- GenoList:   BSU02420
- GeneTree:   EBGT00070000031900
- HOGENOM:   HBG671706
- ProtClustDB:   CLSK916942
- BioCyc:   BSUB:BSU02420-MONOMER
- InterPro:   IPR001463
- PRINTS:   PR00175
- TIGRFAMs:   TIGR00835

Pfam domain/function: PF01235 Na_Ala_symp

EC number: NA

Molecular weight: Translated: 51585; Mature: 51585

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb0340a0)-; HASH(0xc29b054)-; HASH(0xc3be468)-; HASH(0xbf0b15c)-; HASH(0xc294dd8)-; HASH(0xc1d74ec)-; HASH(0xbcabc54)-; HASH(0xc29d4b0)-; HASH(0xc55a208)-; HASH(0xc615bf0)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQILEHIVGIANDLLWSKLLIVLLLSFGIYFTFRLKFLQVRMLKEMVRVLREGAASRSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NSISPFQAFCISMAARVGTGNITGIAIAIALGGPGAIFWMWIIAIIGSASSFVESTLAQI
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHH
YKVKDVNGFRGGPAYYMEKGLNKRWMGALFAVLITLSFGIVFNSVQSNTVSLAFENAFGT
HHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NRLTLGLILIAVFGTIIFGGVKRIAKLAESIVVVLAVLYIGVAFFVIFSNITQLPGVLAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKNAFGFDQAAGGALGAALMQGVRRGIFSNEAGMGSAPNAAATATTSHPVKQGLIQAFG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLTDTLVICTSTAFIILFSDAYHTPGLSGIALTQASLSSHVGSWASGFLAILILLFGFCA
HHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGNYYYGETNIGFLNKSKKLIFVYRIGVLAMIVFGCVAKVQLVWDLADLFMGLMVIVNL
HHCCCEECCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIFLLSKVVFTALKDYTRQKKAGKDPVFYKDVLKNHNGIECWPVSDTKTDTHNKQIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCH
>Mature Secondary Structure
MQQILEHIVGIANDLLWSKLLIVLLLSFGIYFTFRLKFLQVRMLKEMVRVLREGAASRSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NSISPFQAFCISMAARVGTGNITGIAIAIALGGPGAIFWMWIIAIIGSASSFVESTLAQI
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHH
YKVKDVNGFRGGPAYYMEKGLNKRWMGALFAVLITLSFGIVFNSVQSNTVSLAFENAFGT
HHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NRLTLGLILIAVFGTIIFGGVKRIAKLAESIVVVLAVLYIGVAFFVIFSNITQLPGVLAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKNAFGFDQAAGGALGAALMQGVRRGIFSNEAGMGSAPNAAATATTSHPVKQGLIQAFG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLTDTLVICTSTAFIILFSDAYHTPGLSGIALTQASLSSHVGSWASGFLAILILLFGFCA
HHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGNYYYGETNIGFLNKSKKLIFVYRIGVLAMIVFGCVAKVQLVWDLADLFMGLMVIVNL
HHCCCEECCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIFLLSKVVFTALKDYTRQKKAGKDPVFYKDVLKNHNGIECWPVSDTKTDTHNKQIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377