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The map label for this gene is dus [H]

Identifier: 239618483

GI number: 239618483

Start: 2256540

End: 2257484

Strand: Direct

Name: dus [H]

Synonym: Kole_2124

Alternate gene names: 239618483

Gene position: 2256540-2257484 (Clockwise)

Preceding gene: 239618482

Following gene: 239618484

Centisome position: 98.02

GC content: 43.17

Gene sequence:

>945_bases
ATGCCAGAAAATGAAGTCGGTCTTGCACCCATGGCGGGCTATACGGATGAAGCAATGAGGGCCATCTCTCTCGAATGGGG
AGCAGATTTTGTTTTCAGTGAAATGTTGAGCGTTGAAGGAGTTTTGAGAAATGATCGAACCACACAAAAAATCATTCCGG
AAACCCCGTGCAGGATCCAGCTGTTCGGAAATGACATCGCGCGTCTTGCCATGGCTGCAAAAGCCGTTCTTGATAAAGCC
ACATGGTTTGACATTAACGCCGGTTGTCCAGTGAAGAAAGTTGTAAAACGGGGAGCGGGAGCTGCGCTTTTAAGAGAACC
AGAAAAATTAAACGCCATGATTCAGACATTGAAAGGCATTTCAGATTTGCCAGTATCTGTGAAGATCAGGCTTGGGTGGG
ATAAAAACGAGGTGGTAAAGATCGTCGAAAGACTTGTTGAATCCAGACCTTTCGCGATAATGGTTCACGGCCGTACCGTC
GTTCAAGGATATTCTGGTGTTGCAGATTGGGAATCAATCGGTAAAGTGGTAAAAATCTGCAAACCTCATGGAATAAAAGT
TTATGGTTCGGGTGATGTCTTTTCTCCAGAAAAAATTGTAGAAGCTTTCGAAAAGTACGGAGTCGATGGAGTTTTAGTGG
CACGCGGGGCAATTGGAAACCCGTGGATCTTCAGCCAATACAAAGAACTGAAGCAAAAGGGAAGTTACACAGTCCCGGAT
CCTCATGAACGCTTGAAGGTCTTTGCAAAACATCTCAAGAAGCTGATCGAATTAAAGGGAGAAGAAAGAGCGATAATCGA
GTCGAGAAAACACTTTGTGGGTTATTGCAAGGGATTACTAAATGCAACAAAGATGCGAAGAGAATACATGAAATTGAAAA
ACAAAACGGATGTCGAGAATTTTCTTATGCAGTATGGATTAGATATAAAGAGCTTAAAACTATGA

Upstream 100 bases:

>100_bases
ATCGAGATTTCTTCAACTGAAATAAGAGAAAGGATCAAAGAAGGCAAAAGCATTCTGGGAATGGTTCCGTGTTCTATATC
TGAGGAGGTAAGAGCATTTT

Downstream 100 bases:

>100_bases
ATCGCTCAAATAAGCAATTAGGGTGCTCTATTTGTTTTTTGAAAAGAATATTTCATAAGATAAACTAAAAATGAGAGATT
ATGAATAAGGAGGCTGGGGG

Product: dihydrouridine synthase DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPENEVGLAPMAGYTDEAMRAISLEWGADFVFSEMLSVEGVLRNDRTTQKIIPETPCRIQLFGNDIARLAMAAKAVLDKA
TWFDINAGCPVKKVVKRGAGAALLREPEKLNAMIQTLKGISDLPVSVKIRLGWDKNEVVKIVERLVESRPFAIMVHGRTV
VQGYSGVADWESIGKVVKICKPHGIKVYGSGDVFSPEKIVEAFEKYGVDGVLVARGAIGNPWIFSQYKELKQKGSYTVPD
PHERLKVFAKHLKKLIELKGEERAIIESRKHFVGYCKGLLNATKMRREYMKLKNKTDVENFLMQYGLDIKSLKL

Sequences:

>Translated_314_residues
MPENEVGLAPMAGYTDEAMRAISLEWGADFVFSEMLSVEGVLRNDRTTQKIIPETPCRIQLFGNDIARLAMAAKAVLDKA
TWFDINAGCPVKKVVKRGAGAALLREPEKLNAMIQTLKGISDLPVSVKIRLGWDKNEVVKIVERLVESRPFAIMVHGRTV
VQGYSGVADWESIGKVVKICKPHGIKVYGSGDVFSPEKIVEAFEKYGVDGVLVARGAIGNPWIFSQYKELKQKGSYTVPD
PHERLKVFAKHLKKLIELKGEERAIIESRKHFVGYCKGLLNATKMRREYMKLKNKTDVENFLMQYGLDIKSLKL
>Mature_313_residues
PENEVGLAPMAGYTDEAMRAISLEWGADFVFSEMLSVEGVLRNDRTTQKIIPETPCRIQLFGNDIARLAMAAKAVLDKAT
WFDINAGCPVKKVVKRGAGAALLREPEKLNAMIQTLKGISDLPVSVKIRLGWDKNEVVKIVERLVESRPFAIMVHGRTVV
QGYSGVADWESIGKVVKICKPHGIKVYGSGDVFSPEKIVEAFEKYGVDGVLVARGAIGNPWIFSQYKELKQKGSYTVPDP
HERLKVFAKHLKKLIELKGEERAIIESRKHFVGYCKGLLNATKMRREYMKLKNKTDVENFLMQYGLDIKSLKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=278, Percent_Identity=28.4172661870504, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI239788462, Length=278, Percent_Identity=28.4172661870504, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI31742496, Length=261, Percent_Identity=29.1187739463602, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8923374, Length=241, Percent_Identity=29.045643153527, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI40807366, Length=226, Percent_Identity=30.0884955752212, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789660, Length=310, Percent_Identity=29.6774193548387, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788462, Length=232, Percent_Identity=31.8965517241379, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=279, Percent_Identity=27.5985663082437, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=225, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=226, Percent_Identity=26.9911504424779, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=243, Percent_Identity=27.1604938271605, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=26.2222222222222, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=228, Percent_Identity=29.3859649122807, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=300, Percent_Identity=24.6666666666667, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=276, Percent_Identity=28.6231884057971, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=30.2904564315353, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=26.8085106382979, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35168; Mature: 35036

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPENEVGLAPMAGYTDEAMRAISLEWGADFVFSEMLSVEGVLRNDRTTQKIIPETPCRIQ
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEE
LFGNDIARLAMAAKAVLDKATWFDINAGCPVKKVVKRGAGAALLREPEKLNAMIQTLKGI
ECCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCHHHHCCCHHHHHHHHHHHCC
SDLPVSVKIRLGWDKNEVVKIVERLVESRPFAIMVHGRTVVQGYSGVADWESIGKVVKIC
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHC
KPHGIKVYGSGDVFSPEKIVEAFEKYGVDGVLVARGAIGNPWIFSQYKELKQKGSYTVPD
CCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
PHERLKVFAKHLKKLIELKGEERAIIESRKHFVGYCKGLLNATKMRREYMKLKNKTDVEN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FLMQYGLDIKSLKL
HHHHHCCCHHHCCC
>Mature Secondary Structure 
PENEVGLAPMAGYTDEAMRAISLEWGADFVFSEMLSVEGVLRNDRTTQKIIPETPCRIQ
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEE
LFGNDIARLAMAAKAVLDKATWFDINAGCPVKKVVKRGAGAALLREPEKLNAMIQTLKGI
ECCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCHHHHCCCHHHHHHHHHHHCC
SDLPVSVKIRLGWDKNEVVKIVERLVESRPFAIMVHGRTVVQGYSGVADWESIGKVVKIC
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHC
KPHGIKVYGSGDVFSPEKIVEAFEKYGVDGVLVARGAIGNPWIFSQYKELKQKGSYTVPD
CCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
PHERLKVFAKHLKKLIELKGEERAIIESRKHFVGYCKGLLNATKMRREYMKLKNKTDVEN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FLMQYGLDIKSLKL
HHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]