Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 239618428

GI number: 239618428

Start: 2195603

End: 2196577

Strand: Direct

Name: rlmN [H]

Synonym: Kole_2069

Alternate gene names: 239618428

Gene position: 2195603-2196577 (Clockwise)

Preceding gene: 239618427

Following gene: 239618429

Centisome position: 95.37

GC content: 41.23

Gene sequence:

>975_bases
ATGGAATTGGTGGCATGTTACGGCAAGAAGGAACTTGCATTAGTGTTCGTCGGGCGAACTTCGATGGGAAACGAGGTGGA
ATTTGTAGAGTCCATTCAGCCTCCACTGTCCAGGAATGAGAAAGAGGTTATAATAATTTCAACCCTTGTTGGATGCCCTG
TTGGTTGTTCTATGTGTGATGCCGGGGGCTTTTACGGTGGAAAGCTTACCGCCAAGGAAATGCTGGAACAAATCGAATAT
GTCGTAAACTTTAGATTTGGTGGAAGAAAGGTCCCATCAAAGAAGTTCAAAATACAGTTCGCCAGGATGGGAGAACCAGC
TTTCAACGATGCTGTTATCGATGTTCTTGATAGATTGCCAGAAGAGTTCGAGGCTCCGGGCCTCCTTCCCTCTGTATCAA
CTATAGCTCCCAAAGGAAGGGAAGCTTTTTTTGAAAGGCTTTATTGGATAAAAGAACGGCATTACGGGGGAAGATTTCAG
TTACAGTTTTCCATCCATTCTACAGATCTAAGGCAACGAAACGAAATAATACCTGTTCCAAAATGGCACCTTGAAAAAAT
CGCGGAATATGGAAAGGAATTTGTCAAAGAAGGAGATAGAAAGATAACATTGAATTTTGCTCTTTCAAAAGACAACATTG
TTGACCCAGGGATAATAATCAAACACTTCGATCCCGAGAAGTTTCTCATCAAAATCACACCGGTAAATCCTACGTATTCA
GCGAAAGAAAACGGCATTGTATCCGGTGTAGATGTATTTGATAATCGGCTTCCTCATCATCCAGGGTTACTGGAGGCTTT
AGAAAAAAGTGGATACGATGTGATTTTGAGTATCGGAGAGCTTGAAGAAAATAAAATAGGAAGCAACTGTGGACAGTATG
TAAAAAAGCACCTCTCGTCGGATATGGAGCTTTTTGAGGCTTATAGTTATAACAAAATGAGTCTGAATGAAAACAAGAGC
TTTTCACAACCATAA

Upstream 100 bases:

>100_bases
AAAGGGGAAGATTGGGACAGATCTCAATTGCAATTGGTGATGGTGTTGCCGCTGCGATGGATATTATCGGGCTGAGTAAC
TCGTAGGGAGGAACTTGCAC

Downstream 100 bases:

>100_bases
AGGTCATCTTTGGCCTCTATTAAGATTTTACGGTGTAGATGTTTGCAAGTATAATAAAAAGAAATAGTGAATATCTAAAA
TTTTCGTAGAAAGGAATGAT

Product: Fe-S-cluster redox enzyme-like protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MELVACYGKKELALVFVGRTSMGNEVEFVESIQPPLSRNEKEVIIISTLVGCPVGCSMCDAGGFYGGKLTAKEMLEQIEY
VVNFRFGGRKVPSKKFKIQFARMGEPAFNDAVIDVLDRLPEEFEAPGLLPSVSTIAPKGREAFFERLYWIKERHYGGRFQ
LQFSIHSTDLRQRNEIIPVPKWHLEKIAEYGKEFVKEGDRKITLNFALSKDNIVDPGIIIKHFDPEKFLIKITPVNPTYS
AKENGIVSGVDVFDNRLPHHPGLLEALEKSGYDVILSIGELEENKIGSNCGQYVKKHLSSDMELFEAYSYNKMSLNENKS
FSQP

Sequences:

>Translated_324_residues
MELVACYGKKELALVFVGRTSMGNEVEFVESIQPPLSRNEKEVIIISTLVGCPVGCSMCDAGGFYGGKLTAKEMLEQIEY
VVNFRFGGRKVPSKKFKIQFARMGEPAFNDAVIDVLDRLPEEFEAPGLLPSVSTIAPKGREAFFERLYWIKERHYGGRFQ
LQFSIHSTDLRQRNEIIPVPKWHLEKIAEYGKEFVKEGDRKITLNFALSKDNIVDPGIIIKHFDPEKFLIKITPVNPTYS
AKENGIVSGVDVFDNRLPHHPGLLEALEKSGYDVILSIGELEENKIGSNCGQYVKKHLSSDMELFEAYSYNKMSLNENKS
FSQP
>Mature_324_residues
MELVACYGKKELALVFVGRTSMGNEVEFVESIQPPLSRNEKEVIIISTLVGCPVGCSMCDAGGFYGGKLTAKEMLEQIEY
VVNFRFGGRKVPSKKFKIQFARMGEPAFNDAVIDVLDRLPEEFEAPGLLPSVSTIAPKGREAFFERLYWIKERHYGGRFQ
LQFSIHSTDLRQRNEIIPVPKWHLEKIAEYGKEFVKEGDRKITLNFALSKDNIVDPGIIIKHFDPEKFLIKITPVNPTYS
AKENGIVSGVDVFDNRLPHHPGLLEALEKSGYDVILSIGELEENKIGSNCGQYVKKHLSSDMELFEAYSYNKMSLNENKS
FSQP

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 36609; Mature: 36609

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVACYGKKELALVFVGRTSMGNEVEFVESIQPPLSRNEKEVIIISTLVGCPVGCSMCD
CCEEEECCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHC
AGGFYGGKLTAKEMLEQIEYVVNFRFGGRKVPSKKFKIQFARMGEPAFNDAVIDVLDRLP
CCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCHHEEEEEEECCCCCCHHHHHHHHHHCH
EEFEAPGLLPSVSTIAPKGREAFFERLYWIKERHYGGRFQLQFSIHSTDLRQRNEIIPVP
HHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHCCCCCCCC
KWHLEKIAEYGKEFVKEGDRKITLNFALSKDNIVDPGIIIKHFDPEKFLIKITPVNPTYS
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCCEEEEEEECCCCCCC
AKENGIVSGVDVFDNRLPHHPGLLEALEKSGYDVILSIGELEENKIGSNCGQYVKKHLSS
CCCCCCEECHHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHCCHHHHHHHHHHHCC
DMELFEAYSYNKMSLNENKSFSQP
HHHHHHHHCCCCEECCCCCCCCCC
>Mature Secondary Structure
MELVACYGKKELALVFVGRTSMGNEVEFVESIQPPLSRNEKEVIIISTLVGCPVGCSMCD
CCEEEECCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHC
AGGFYGGKLTAKEMLEQIEYVVNFRFGGRKVPSKKFKIQFARMGEPAFNDAVIDVLDRLP
CCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCHHEEEEEEECCCCCCHHHHHHHHHHCH
EEFEAPGLLPSVSTIAPKGREAFFERLYWIKERHYGGRFQLQFSIHSTDLRQRNEIIPVP
HHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHCCCCCCCC
KWHLEKIAEYGKEFVKEGDRKITLNFALSKDNIVDPGIIIKHFDPEKFLIKITPVNPTYS
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCCEEEEEEECCCCCCC
AKENGIVSGVDVFDNRLPHHPGLLEALEKSGYDVILSIGELEENKIGSNCGQYVKKHLSS
CCCCCCEECHHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHCCHHHHHHHHHHHCC
DMELFEAYSYNKMSLNENKSFSQP
HHHHHHHHCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA