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The map label for this gene is trxB [C]

Identifier: 239618427

GI number: 239618427

Start: 2194749

End: 2195588

Strand: Direct

Name: trxB [C]

Synonym: Kole_2068

Alternate gene names: 239618427

Gene position: 2194749-2195588 (Clockwise)

Preceding gene: 239618425

Following gene: 239618428

Centisome position: 95.34

GC content: 40.12

Gene sequence:

>840_bases
ATGTTTAGAACAAAAGTCGCAATAATTGGTGCCGGTCCCGCCGGGATTTCTTGTGCGATACAACTGAAACATGCAGGTGT
TGACTTTGAAATCTACGAAAAGTCAGAAATCGGGGGAACAGTCAAAAATGCCAACCTTATAGAGAACGTACCAGGGTTTG
ATCCGTTAACTGGGGAAGAGTTTGCAAACAAGCTCGAAAAACATCTTCAAAGACTTAAAATTGTAGTGAATATGTCTCAA
ATAAATAAGATTCAACATCAAGATAACTTTTTCATTCTCGAAGCGAATGACTGTATGGTAGTTTCAGAGTACGTGGTTCT
AGCAACAGGAACGGTTCCCAGAACCATGCCGGATTTTGAGGTGCCAGAAAGAACGTTTTATGAGGTCAGAAAAATGCGCC
ATATTAAAAACTCAACGATAGCCGTTATAGGTTCGGGAGAAGCTGCCTACGATAGTGCATTGAATCTTACTAAAAATCGA
AATCGTGTTCACTTGTTTACAAAAAAAGAACCTCAGAATGTGGCTAAACGCCTTTTAAAAAAGGTTATATCAAATGATTT
TGTCGAGGTTCATTTGAAAGAACCTGTTGTTGAAATCTTGAAGATAGAGGATAATTGGTATGGTGCTCGTACTGATAGGG
GAATATACGTCGTTGACTATTTTCTGATCAGTATCGGGAGAGTACCTAATCTTGCCCTTCTTAAAGGATTGCATTCTGAT
TCATTGACCAATCTGTTCCTGTGTGGTGATGCTAAAAGGGGAAGATTGGGACAGATCTCAATTGCAATTGGTGATGGTGT
TGCCGCTGCGATGGATATTATCGGGCTGAGTAACTCGTAG

Upstream 100 bases:

>100_bases
TGGACGCTGTAGGTTCCAGGAACGATCTTTTTCATCTTTCAGGACTTGTAACGCTGGCACATGGTTTTTCTACTTTGATC
ACAACCGGGGAGCTGATTGA

Downstream 100 bases:

>100_bases
GGAGGAACTTGCACATGGAATTGGTGGCATGTTACGGCAAGAAGGAACTTGCATTAGTGTTCGTCGGGCGAACTTCGATG
GGAAACGAGGTGGAATTTGT

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MFRTKVAIIGAGPAGISCAIQLKHAGVDFEIYEKSEIGGTVKNANLIENVPGFDPLTGEEFANKLEKHLQRLKIVVNMSQ
INKIQHQDNFFILEANDCMVVSEYVVLATGTVPRTMPDFEVPERTFYEVRKMRHIKNSTIAVIGSGEAAYDSALNLTKNR
NRVHLFTKKEPQNVAKRLLKKVISNDFVEVHLKEPVVEILKIEDNWYGARTDRGIYVVDYFLISIGRVPNLALLKGLHSD
SLTNLFLCGDAKRGRLGQISIAIGDGVAAAMDIIGLSNS

Sequences:

>Translated_279_residues
MFRTKVAIIGAGPAGISCAIQLKHAGVDFEIYEKSEIGGTVKNANLIENVPGFDPLTGEEFANKLEKHLQRLKIVVNMSQ
INKIQHQDNFFILEANDCMVVSEYVVLATGTVPRTMPDFEVPERTFYEVRKMRHIKNSTIAVIGSGEAAYDSALNLTKNR
NRVHLFTKKEPQNVAKRLLKKVISNDFVEVHLKEPVVEILKIEDNWYGARTDRGIYVVDYFLISIGRVPNLALLKGLHSD
SLTNLFLCGDAKRGRLGQISIAIGDGVAAAMDIIGLSNS
>Mature_279_residues
MFRTKVAIIGAGPAGISCAIQLKHAGVDFEIYEKSEIGGTVKNANLIENVPGFDPLTGEEFANKLEKHLQRLKIVVNMSQ
INKIQHQDNFFILEANDCMVVSEYVVLATGTVPRTMPDFEVPERTFYEVRKMRHIKNSTIAVIGSGEAAYDSALNLTKNR
NRVHLFTKKEPQNVAKRLLKKVISNDFVEVHLKEPVVEILKIEDNWYGARTDRGIYVVDYFLISIGRVPNLALLKGLHSD
SLTNLFLCGDAKRGRLGQISIAIGDGVAAAMDIIGLSNS

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=307, Percent_Identity=24.1042345276873, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320560, Length=316, Percent_Identity=25.3164556962025, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321898, Length=316, Percent_Identity=25, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 30939; Mature: 30939

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTKVAIIGAGPAGISCAIQLKHAGVDFEIYEKSEIGGTVKNANLIENVPGFDPLTGEE
CCCEEEEEEECCCCCEEEEEEEEECCCCEEEEECCCCCCEECCCHHHHCCCCCCCCCHHH
FANKLEKHLQRLKIVVNMSQINKIQHQDNFFILEANDCMVVSEYVVLATGTVPRTMPDFE
HHHHHHHHHHHHHHEEEHHHHHHHHCCCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCC
VPERTFYEVRKMRHIKNSTIAVIGSGEAAYDSALNLTKNRNRVHLFTKKEPQNVAKRLLK
CCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
KVISNDFVEVHLKEPVVEILKIEDNWYGARTDRGIYVVDYFLISIGRVPNLALLKGLHSD
HHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEEEHHHHHCCCCCHHHHHCCCCC
SLTNLFLCGDAKRGRLGQISIAIGDGVAAAMDIIGLSNS
CCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHEECCCC
>Mature Secondary Structure
MFRTKVAIIGAGPAGISCAIQLKHAGVDFEIYEKSEIGGTVKNANLIENVPGFDPLTGEE
CCCEEEEEEECCCCCEEEEEEEEECCCCEEEEECCCCCCEECCCHHHHCCCCCCCCCHHH
FANKLEKHLQRLKIVVNMSQINKIQHQDNFFILEANDCMVVSEYVVLATGTVPRTMPDFE
HHHHHHHHHHHHHHEEEHHHHHHHHCCCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCC
VPERTFYEVRKMRHIKNSTIAVIGSGEAAYDSALNLTKNRNRVHLFTKKEPQNVAKRLLK
CCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
KVISNDFVEVHLKEPVVEILKIEDNWYGARTDRGIYVVDYFLISIGRVPNLALLKGLHSD
HHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEEEHHHHHCCCCCHHHHHCCCCC
SLTNLFLCGDAKRGRLGQISIAIGDGVAAAMDIIGLSNS
CCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]