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The map label for this gene is rho [H]

Identifier: 239618234

GI number: 239618234

Start: 2006775

End: 2008181

Strand: Reverse

Name: rho [H]

Synonym: Kole_1871

Alternate gene names: 239618234

Gene position: 2008181-2006775 (Counterclockwise)

Preceding gene: 239618235

Following gene: 239618233

Centisome position: 87.23

GC content: 42.43

Gene sequence:

>1407_bases
GTGAGCTCTTCCGAGCAGAATCATTCTGAGAAGGTAAACAAAACTCCTGAAACTTCCGCCAACAAAGATTCTTCCACAAC
AGATAAAACTGACAACGAAAAAGACCAGAACAACAAGGTCAAGGATACTATCAGGGAAGTGGAAATCGACATTTCCCAGC
TTCTCAAAATGCCCATACGTGAACTCTACAAACTCGCGAGAAAATACTCCATCACCGGTTATACACAAATGGCAAAGAAA
GATCTTATCTTTGCTGTGCTCAAAGCACAAACCGAATCTTATGGATACTTCTTTGCTGAAGGTGTGCTTGAGATAACTGA
CGGGGGCTATGGGTTTCTTAGAACCCTTGATAACAATCTCTTACCAAGTTCTAACGATATATACGTCAGTCAATCTCAGA
TTCGCAGATTCAACCTGAATACGGGTGATTGGGTTGCGGGTCAGGTCCGTTCTCCCAAAGAAGGAGAAAAGTATTTTGCA
CTGTTGAGAATCGAAGCAATCAATCAAAAACCCGTGTCTTACGCTGAAGAGAGAATAAGTTTCCAGAATCTTGTTCCTGT
TTATCCGGATGAAAAGTTCATTCTCGAAACGGAACCATATATTGTTAGCACAAGGATAATTGATTTGTTTTCTCCAATCG
GCAAAGGCCAGAGAGGGCTTATCGTCGCACCACCTAAAGCTGGAAAAACCACTCTGCTCAAAGAGATCGCCAATGGAATC
GCGGAAAATCATCCAAACACAATACGAATTATTCTTTTGATAGATGAACGCCCAGAAGAGGTTACCGACTTACGAAGGTC
AACTGATGCTCACGTAATAGCTGCCCCATTTGATATGTCACCTGACAAACAGATAAGAGTTGCAGAGTTGACACTGGAAA
TGGCCAAGCGTCTGGTCGAGTTTGGCTACGACGTCGTGATATTGCTGGACAGTATCACTCGTCTTGCAAGGGCATACAAC
CTCTACGTTCCCCCAAGTGGAAAACTTCTCAGTGGTGGTGTCGACCCATCTGCACTGTATCATCCAAAGCATTTCTTTGG
TGCTGCCAGGAATATTGAAGACGGAGGAAGTTTGACCATAATATCCACCGCGTTGGTTGAAACTGGAAGCAAGATGGATG
AAGTTATTTTTGAAGAATTCAAAGGCACTGGAAATATGGAATTGGTTCTCTCTAGACAACTCGCCAACAAACGAATATTC
CCTGCAATAAATCTAAACCTTTCCGGAACAAGGAAGGAAGAACTCCTGCTTCCGCCTGAAATTCTAAGGAAAGTTTGGGT
TCTCAGAAGAATGCTTGCGGGAATCAGTGAAGAGGAAGGATTAAAAGTCATTGTAGATAAAATGAAAACCACTAAATCCA
ACGAGGAATTTCTTGATCTGATAGATCTCGAAAAAAGCAGATATTGA

Upstream 100 bases:

>100_bases
TCCGTTTATTCTGGGGATTGCACCGTGAAAATTGAGCCCAAGAAGGAAAAAAAGTGTTGAAAAATCTTCTGAAGAAAAGG
AAGTTAAGGAAGTCGAAAGT

Downstream 100 bases:

>100_bases
AAGGTAGGATGAAATGGGATTAGTTATTGGCATCGATCTTGGAGGTACAGAAACTAAAATAGGTTTGGTAGATGAACATG
GAAAGATTCTGAACAAAACA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSSSEQNHSEKVNKTPETSANKDSSTTDKTDNEKDQNNKVKDTIREVEIDISQLLKMPIRELYKLARKYSITGYTQMAKK
DLIFAVLKAQTESYGYFFAEGVLEITDGGYGFLRTLDNNLLPSSNDIYVSQSQIRRFNLNTGDWVAGQVRSPKEGEKYFA
LLRIEAINQKPVSYAEERISFQNLVPVYPDEKFILETEPYIVSTRIIDLFSPIGKGQRGLIVAPPKAGKTTLLKEIANGI
AENHPNTIRIILLIDERPEEVTDLRRSTDAHVIAAPFDMSPDKQIRVAELTLEMAKRLVEFGYDVVILLDSITRLARAYN
LYVPPSGKLLSGGVDPSALYHPKHFFGAARNIEDGGSLTIISTALVETGSKMDEVIFEEFKGTGNMELVLSRQLANKRIF
PAINLNLSGTRKEELLLPPEILRKVWVLRRMLAGISEEEGLKVIVDKMKTTKSNEEFLDLIDLEKSRY

Sequences:

>Translated_468_residues
MSSSEQNHSEKVNKTPETSANKDSSTTDKTDNEKDQNNKVKDTIREVEIDISQLLKMPIRELYKLARKYSITGYTQMAKK
DLIFAVLKAQTESYGYFFAEGVLEITDGGYGFLRTLDNNLLPSSNDIYVSQSQIRRFNLNTGDWVAGQVRSPKEGEKYFA
LLRIEAINQKPVSYAEERISFQNLVPVYPDEKFILETEPYIVSTRIIDLFSPIGKGQRGLIVAPPKAGKTTLLKEIANGI
AENHPNTIRIILLIDERPEEVTDLRRSTDAHVIAAPFDMSPDKQIRVAELTLEMAKRLVEFGYDVVILLDSITRLARAYN
LYVPPSGKLLSGGVDPSALYHPKHFFGAARNIEDGGSLTIISTALVETGSKMDEVIFEEFKGTGNMELVLSRQLANKRIF
PAINLNLSGTRKEELLLPPEILRKVWVLRRMLAGISEEEGLKVIVDKMKTTKSNEEFLDLIDLEKSRY
>Mature_467_residues
SSSEQNHSEKVNKTPETSANKDSSTTDKTDNEKDQNNKVKDTIREVEIDISQLLKMPIRELYKLARKYSITGYTQMAKKD
LIFAVLKAQTESYGYFFAEGVLEITDGGYGFLRTLDNNLLPSSNDIYVSQSQIRRFNLNTGDWVAGQVRSPKEGEKYFAL
LRIEAINQKPVSYAEERISFQNLVPVYPDEKFILETEPYIVSTRIIDLFSPIGKGQRGLIVAPPKAGKTTLLKEIANGIA
ENHPNTIRIILLIDERPEEVTDLRRSTDAHVIAAPFDMSPDKQIRVAELTLEMAKRLVEFGYDVVILLDSITRLARAYNL
YVPPSGKLLSGGVDPSALYHPKHFFGAARNIEDGGSLTIISTALVETGSKMDEVIFEEFKGTGNMELVLSRQLANKRIFP
AINLNLSGTRKEELLLPPEILRKVWVLRRMLAGISEEEGLKVIVDKMKTTKSNEEFLDLIDLEKSRY

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=53.11004784689, Blast_Score=460, Evalue=1e-131,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 52755; Mature: 52624

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSEQNHSEKVNKTPETSANKDSSTTDKTDNEKDQNNKVKDTIREVEIDISQLLKMPIR
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ELYKLARKYSITGYTQMAKKDLIFAVLKAQTESYGYFFAEGVLEITDGGYGFLRTLDNNL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCHHHHHHCCCC
LPSSNDIYVSQSQIRRFNLNTGDWVAGQVRSPKEGEKYFALLRIEAINQKPVSYAEERIS
CCCCCCEEEEHHHHHEECCCCCCEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHH
FQNLVPVYPDEKFILETEPYIVSTRIIDLFSPIGKGQRGLIVAPPKAGKTTLLKEIANGI
HHHCCCCCCCCCEEEECCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
AENHPNTIRIILLIDERPEEVTDLRRSTDAHVIAAPFDMSPDKQIRVAELTLEMAKRLVE
HHCCCCEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHH
FGYDVVILLDSITRLARAYNLYVPPSGKLLSGGVDPSALYHPKHFFGAARNIEDGGSLTI
CCCCEEHHHHHHHHHHHHHEEEECCCCCEEECCCCCHHHCCCHHHHHHHCCCCCCCCEEE
ISTALVETGSKMDEVIFEEFKGTGNMELVLSRQLANKRIFPAINLNLSGTRKEELLLPPE
EEHHHHHCCCHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCEEEEECCCCCCCCCCCCCHH
ILRKVWVLRRMLAGISEEEGLKVIVDKMKTTKSNEEFLDLIDLEKSRY
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSSEQNHSEKVNKTPETSANKDSSTTDKTDNEKDQNNKVKDTIREVEIDISQLLKMPIR
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ELYKLARKYSITGYTQMAKKDLIFAVLKAQTESYGYFFAEGVLEITDGGYGFLRTLDNNL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCHHHHHHCCCC
LPSSNDIYVSQSQIRRFNLNTGDWVAGQVRSPKEGEKYFALLRIEAINQKPVSYAEERIS
CCCCCCEEEEHHHHHEECCCCCCEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHH
FQNLVPVYPDEKFILETEPYIVSTRIIDLFSPIGKGQRGLIVAPPKAGKTTLLKEIANGI
HHHCCCCCCCCCEEEECCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
AENHPNTIRIILLIDERPEEVTDLRRSTDAHVIAAPFDMSPDKQIRVAELTLEMAKRLVE
HHCCCCEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHH
FGYDVVILLDSITRLARAYNLYVPPSGKLLSGGVDPSALYHPKHFFGAARNIEDGGSLTI
CCCCEEHHHHHHHHHHHHHEEEECCCCCEEECCCCCHHHCCCHHHHHHHCCCCCCCCEEE
ISTALVETGSKMDEVIFEEFKGTGNMELVLSRQLANKRIFPAINLNLSGTRKEELLLPPE
EEHHHHHCCCHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCEEEEECCCCCCCCCCCCCHH
ILRKVWVLRRMLAGISEEEGLKVIVDKMKTTKSNEEFLDLIDLEKSRY
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8051015; 10360571 [H]