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The map label for this gene is pnp [H]

Identifier: 239618017

GI number: 239618017

Start: 1766834

End: 1769047

Strand: Direct

Name: pnp [H]

Synonym: Kole_1646

Alternate gene names: 239618017

Gene position: 1766834-1769047 (Clockwise)

Preceding gene: 239618016

Following gene: 239618018

Centisome position: 76.75

GC content: 44.85

Gene sequence:

>2214_bases
ATGAACGATTATCGTGTTTGGGAAAGGGAATTTTTTGGAAGGAAAATCATTGTGGAACACGGAAAAATGGCGAAGCAGTC
TCATGGTGCCTGTTTTGTTCGATTTGCAGATTCTGCCATTCTTGCAACGGTAAATGCCAGTGAGGAGCCTCTTCCTCAAG
CTGGTTTTGTACCGCTTACGGTCGAGTTTCAGGAGAAATTCTACGCTGCCGGTAAGATTCCCGGTGGTTTTGTTAAGAGA
GAGGGGAAACCAAGTGAAAACGCCATACTATCAGCGAGGCTGATAGATAGGCCTATCAGGCCGCTGTTTCCGAAGGGTAT
GTCCAATGAGGTTCAGGTTATCGTGACGGTTCTTTCCGCAGATCCCGATAATCCGCCTGACACAATTGGAATATTTGCAA
GTTCGTTAGCCTTGAACATATCGCCTATTCCATTTGACGGTATGGTTGCCGGTGTCAGGGTTGGCTATGTTGACGGCGAG
TATATATTCTTCCCGACCAGTGAAGAACTCGAGAAGAGCGAACTTGATATAGTGGTTGCCGGTACAAAAGAAGCCGTTAC
TATGGTTGAAGGCGAAGCGAAAGAGGTTTCCGAAGAAGTCATGTTGAAAGCGCTCATGAAAGCTCATGAGGCAATCAAGC
AACTTGTTGAATTTCAGGAAGAAATTGTTTCGGAGTTCGAGGTTCAAAAATGGGAACTGGAAGTACCCGAACCACCCGAA
GGTTTCATAGAAGCTTTCGAATCCCTCTTGGATGATCAGGAAATCATTGAAAGAATGCTCACACCAGGAAAGAAAAATAA
AGGTAGAGCCCTTAAAGAATATAGAAATGCGATCATTGAAGAGATGAAAGAGAAATTTACAGATAAGTGGTCTGAAGATG
AGTTTGTCGAAAACGAGCATTTCCTCAAAGAACATTATGAAGAAAAAGTAAAACATCTCATGCGTCGTTCAATTGTTGAG
CGCAATTTAAGGATGGACGGTAGAAAGACTACCGAGATTCGCCCGATAACATGCGAACTGGACATTCTTCCAAGGACGCA
CGGTTCTGCTCTGTTTACCAGAGGGGAAACTCAGAGTCTTGCAACTGTCACACTTGGGGCACCGATAGACGAGCAGATAA
TCGATACCCTGTTGGAGGAAGGCTCAAAAACTTTCATGCTTCATTACAACTTCCCACCTTTCAGCACCGGTGAAGTCAAA
AGAATGAGGGGACCTGGAAGAAGGGAGATAGGGCATGGCCATCTTGCGGAAAGGGCCCTTAAAAATATGATTCCGCCTGA
AGAAGAATTTCCTTACACGATCAGGGTAGTTTCTGAAATTCTGGAATCCAATGGTTCCTCGTCTATGGCGACGGTCTGCT
CCGGTTCTCTTGCCCTTATGGCTGCTGGTGTTCCTATAAAAAGGCATGTTGCCGGTGTGGCAATGGGTTTGATTCAGGAG
CCGGATGAAACCGTGATTCTTACGGATATTCTTGGTAACGAGGACCATTATGGAGATATGGATTTCAAAGTCGCGGGAAC
TCGCGACGGTATAACGGCCTTCCAGATGGACGTTAAAGTTGCCGGCGTTTCAGAAGAGATAATGCTTAAGGCTCTTTATC
AGGCAAAAGAAGCTAGGATGAAAATACTCGATCTCATGTACGAAACCATTTCGGAGCCGAGAAAGGAGATCTCTCCATAC
GCACCGGTAATTAAAGTAATGGCTGTTCCGTACGAAAAAATCGGCGAGATTATCGGACCTGGTGGAAAGGTTGTTAAAAA
GTTGAGCACCGATTACGATGCAAGAATATTCATAGATGATGTGAAATGCCAGGTCAAAATAATAGGTAACGATCCGGAGA
AGGTTGAAAAACTCATGGGAGTTATCAACGCTGTCATTTCCGATGTCAAAGAGGGGCAGGTTTTCGATGGCGTGATTTCC
AGAATAGAACCTTACGGTATTTTCGTAGAAATCGCTCCCGGTAAGACAGGTCTTCTCCATTCCTCTAAATTCGGTGGAGA
TATGAAGGAATTTATCAAAACGAATAATGTTGGTGCACACCTCAAGGTTGAGGTAACTGCCATCGATAATCTCGGAAGAA
TTCAATTGAAGAGATATGGTGTAGAACTTACAGACGAGCAGAAAAAGTCGCGTGGAAGGAGCTCCGGCAGACCTTCACAC
TACGGCAATAGAAGACCAAACGGGCACAAAAAAGAGGGCGGAAGAAATCAATGA

Upstream 100 bases:

>100_bases
TATAATACTCAAGGGGGAGCGGACAGCCGCTCCCTCTTTTACTTGTTTTATGAATAACTGATATAATAAAGATGGATAAA
AGGAAAAAAGAGGTGTTAAA

Downstream 100 bases:

>100_bases
GAAGCACTGCAAATGTTAAACTCGTGAAATTAGATAACGGATCCACAATCATCTTTGAAAAGAAGAGTGACACCAGAACG
GTGTCACTTGCTTTTGCGGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MNDYRVWEREFFGRKIIVEHGKMAKQSHGACFVRFADSAILATVNASEEPLPQAGFVPLTVEFQEKFYAAGKIPGGFVKR
EGKPSENAILSARLIDRPIRPLFPKGMSNEVQVIVTVLSADPDNPPDTIGIFASSLALNISPIPFDGMVAGVRVGYVDGE
YIFFPTSEELEKSELDIVVAGTKEAVTMVEGEAKEVSEEVMLKALMKAHEAIKQLVEFQEEIVSEFEVQKWELEVPEPPE
GFIEAFESLLDDQEIIERMLTPGKKNKGRALKEYRNAIIEEMKEKFTDKWSEDEFVENEHFLKEHYEEKVKHLMRRSIVE
RNLRMDGRKTTEIRPITCELDILPRTHGSALFTRGETQSLATVTLGAPIDEQIIDTLLEEGSKTFMLHYNFPPFSTGEVK
RMRGPGRREIGHGHLAERALKNMIPPEEEFPYTIRVVSEILESNGSSSMATVCSGSLALMAAGVPIKRHVAGVAMGLIQE
PDETVILTDILGNEDHYGDMDFKVAGTRDGITAFQMDVKVAGVSEEIMLKALYQAKEARMKILDLMYETISEPRKEISPY
APVIKVMAVPYEKIGEIIGPGGKVVKKLSTDYDARIFIDDVKCQVKIIGNDPEKVEKLMGVINAVISDVKEGQVFDGVIS
RIEPYGIFVEIAPGKTGLLHSSKFGGDMKEFIKTNNVGAHLKVEVTAIDNLGRIQLKRYGVELTDEQKKSRGRSSGRPSH
YGNRRPNGHKKEGGRNQ

Sequences:

>Translated_737_residues
MNDYRVWEREFFGRKIIVEHGKMAKQSHGACFVRFADSAILATVNASEEPLPQAGFVPLTVEFQEKFYAAGKIPGGFVKR
EGKPSENAILSARLIDRPIRPLFPKGMSNEVQVIVTVLSADPDNPPDTIGIFASSLALNISPIPFDGMVAGVRVGYVDGE
YIFFPTSEELEKSELDIVVAGTKEAVTMVEGEAKEVSEEVMLKALMKAHEAIKQLVEFQEEIVSEFEVQKWELEVPEPPE
GFIEAFESLLDDQEIIERMLTPGKKNKGRALKEYRNAIIEEMKEKFTDKWSEDEFVENEHFLKEHYEEKVKHLMRRSIVE
RNLRMDGRKTTEIRPITCELDILPRTHGSALFTRGETQSLATVTLGAPIDEQIIDTLLEEGSKTFMLHYNFPPFSTGEVK
RMRGPGRREIGHGHLAERALKNMIPPEEEFPYTIRVVSEILESNGSSSMATVCSGSLALMAAGVPIKRHVAGVAMGLIQE
PDETVILTDILGNEDHYGDMDFKVAGTRDGITAFQMDVKVAGVSEEIMLKALYQAKEARMKILDLMYETISEPRKEISPY
APVIKVMAVPYEKIGEIIGPGGKVVKKLSTDYDARIFIDDVKCQVKIIGNDPEKVEKLMGVINAVISDVKEGQVFDGVIS
RIEPYGIFVEIAPGKTGLLHSSKFGGDMKEFIKTNNVGAHLKVEVTAIDNLGRIQLKRYGVELTDEQKKSRGRSSGRPSH
YGNRRPNGHKKEGGRNQ
>Mature_737_residues
MNDYRVWEREFFGRKIIVEHGKMAKQSHGACFVRFADSAILATVNASEEPLPQAGFVPLTVEFQEKFYAAGKIPGGFVKR
EGKPSENAILSARLIDRPIRPLFPKGMSNEVQVIVTVLSADPDNPPDTIGIFASSLALNISPIPFDGMVAGVRVGYVDGE
YIFFPTSEELEKSELDIVVAGTKEAVTMVEGEAKEVSEEVMLKALMKAHEAIKQLVEFQEEIVSEFEVQKWELEVPEPPE
GFIEAFESLLDDQEIIERMLTPGKKNKGRALKEYRNAIIEEMKEKFTDKWSEDEFVENEHFLKEHYEEKVKHLMRRSIVE
RNLRMDGRKTTEIRPITCELDILPRTHGSALFTRGETQSLATVTLGAPIDEQIIDTLLEEGSKTFMLHYNFPPFSTGEVK
RMRGPGRREIGHGHLAERALKNMIPPEEEFPYTIRVVSEILESNGSSSMATVCSGSLALMAAGVPIKRHVAGVAMGLIQE
PDETVILTDILGNEDHYGDMDFKVAGTRDGITAFQMDVKVAGVSEEIMLKALYQAKEARMKILDLMYETISEPRKEISPY
APVIKVMAVPYEKIGEIIGPGGKVVKKLSTDYDARIFIDDVKCQVKIIGNDPEKVEKLMGVINAVISDVKEGQVFDGVIS
RIEPYGIFVEIAPGKTGLLHSSKFGGDMKEFIKTNNVGAHLKVEVTAIDNLGRIQLKRYGVELTDEQKKSRGRSSGRPSH
YGNRRPNGHKKEGGRNQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=678, Percent_Identity=37.0206489675516, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=45.095168374817, Blast_Score=573, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI115534063, Length=679, Percent_Identity=36.0824742268041, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI281362905, Length=678, Percent_Identity=38.0530973451327, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=678, Percent_Identity=38.0530973451327, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=678, Percent_Identity=38.0530973451327, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=623, Percent_Identity=38.5232744783307, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 82274; Mature: 82274

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYRVWEREFFGRKIIVEHGKMAKQSHGACFVRFADSAILATVNASEEPLPQAGFVPLT
CCCCHHHHHHHCCCEEEECCCCHHHHCCCEEEEEEECCEEEEEECCCCCCCCCCCCEEEE
VEFQEKFYAAGKIPGGFVKREGKPSENAILSARLIDRPIRPLFPKGMSNEVQVIVTVLSA
EEHHHHHHHCCCCCCCHHCCCCCCCCHHEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEC
DPDNPPDTIGIFASSLALNISPIPFDGMVAGVRVGYVDGEYIFFPTSEELEKSELDIVVA
CCCCCCCHHHHHHHHHEEECCCCCCCCEEECEEEEEECCCEEEECCHHHHCCCCCCEEEE
GTKEAVTMVEGEAKEVSEEVMLKALMKAHEAIKQLVEFQEEIVSEFEVQKWELEVPEPPE
CCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
GFIEAFESLLDDQEIIERMLTPGKKNKGRALKEYRNAIIEEMKEKFTDKWSEDEFVENEH
HHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FLKEHYEEKVKHLMRRSIVERNLRMDGRKTTEIRPITCELDILPRTHGSALFTRGETQSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCCE
ATVTLGAPIDEQIIDTLLEEGSKTFMLHYNFPPFSTGEVKRMRGPGRREIGHGHLAERAL
EEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCHHHHHHH
KNMIPPEEEFPYTIRVVSEILESNGSSSMATVCSGSLALMAAGVPIKRHVAGVAMGLIQE
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHCC
PDETVILTDILGNEDHYGDMDFKVAGTRDGITAFQMDVKVAGVSEEIMLKALYQAKEARM
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHH
KILDLMYETISEPRKEISPYAPVIKVMAVPYEKIGEIIGPGGKVVKKLSTDYDARIFIDD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEE
VKCQVKIIGNDPEKVEKLMGVINAVISDVKEGQVFDGVISRIEPYGIFVEIAPGKTGLLH
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCC
SSKFGGDMKEFIKTNNVGAHLKVEVTAIDNLGRIQLKRYGVELTDEQKKSRGRSSGRPSH
CCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCCCCC
YGNRRPNGHKKEGGRNQ
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDYRVWEREFFGRKIIVEHGKMAKQSHGACFVRFADSAILATVNASEEPLPQAGFVPLT
CCCCHHHHHHHCCCEEEECCCCHHHHCCCEEEEEEECCEEEEEECCCCCCCCCCCCEEEE
VEFQEKFYAAGKIPGGFVKREGKPSENAILSARLIDRPIRPLFPKGMSNEVQVIVTVLSA
EEHHHHHHHCCCCCCCHHCCCCCCCCHHEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEC
DPDNPPDTIGIFASSLALNISPIPFDGMVAGVRVGYVDGEYIFFPTSEELEKSELDIVVA
CCCCCCCHHHHHHHHHEEECCCCCCCCEEECEEEEEECCCEEEECCHHHHCCCCCCEEEE
GTKEAVTMVEGEAKEVSEEVMLKALMKAHEAIKQLVEFQEEIVSEFEVQKWELEVPEPPE
CCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
GFIEAFESLLDDQEIIERMLTPGKKNKGRALKEYRNAIIEEMKEKFTDKWSEDEFVENEH
HHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FLKEHYEEKVKHLMRRSIVERNLRMDGRKTTEIRPITCELDILPRTHGSALFTRGETQSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCCE
ATVTLGAPIDEQIIDTLLEEGSKTFMLHYNFPPFSTGEVKRMRGPGRREIGHGHLAERAL
EEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCHHHHHHH
KNMIPPEEEFPYTIRVVSEILESNGSSSMATVCSGSLALMAAGVPIKRHVAGVAMGLIQE
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHCC
PDETVILTDILGNEDHYGDMDFKVAGTRDGITAFQMDVKVAGVSEEIMLKALYQAKEARM
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHH
KILDLMYETISEPRKEISPYAPVIKVMAVPYEKIGEIIGPGGKVVKKLSTDYDARIFIDD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEE
VKCQVKIIGNDPEKVEKLMGVINAVISDVKEGQVFDGVISRIEPYGIFVEIAPGKTGLLH
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCC
SSKFGGDMKEFIKTNNVGAHLKVEVTAIDNLGRIQLKRYGVELTDEQKKSRGRSSGRPSH
CCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCCCCC
YGNRRPNGHKKEGGRNQ
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]