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The map label for this gene is tilS [H]

Identifier: 239617939

GI number: 239617939

Start: 1678805

End: 1680214

Strand: Direct

Name: tilS [H]

Synonym: Kole_1567

Alternate gene names: 239617939

Gene position: 1678805-1680214 (Clockwise)

Preceding gene: 239617938

Following gene: 239617940

Centisome position: 72.92

GC content: 39.22

Gene sequence:

>1410_bases
ATGACCGGGTTTGAAAAGAAGGTATTTGAGTTCATAGATCGATGGGGACTTATAAAGCAAGGAGAAAAAATTCTGGTGGC
AGTATCCGGCGGTAAAGATTCTATGGCTCTGCTCCACTTTTTGGTTTCTAAAAAAGAACGATTGGGATGTGAGATCTGCG
TTGCGAATATGGATCACGGCTTACGTGATGACAGCGGTCGCGAGGAAGCCGTGATGATAGCCTCCTTTTGTGAAAAACAT
GATGTTCCTTTTTATCATGAGAGACGCGACGTAACCCAATATCATAAAAATAACCGTGGGCTTTCTCTTGAAGAAGCGGC
ACGTATTGTTCGATACGATTTTTTGTTCAATGCCAGAGAAAAACTTGGAGCCGATAAGATCGCCCTGGCCCATCATCTGA
ACGATTTGGTGGAGACGATGTTGATGAGGATAGCAAGAGGAACGGGGTTAAAAGGGGTAATTTCTATGAAGCCTATGAAC
AATTCTATAATACGTCCTCTCTTATCTGTTGGAGTGCAAGAAATACTCGATTATGTTACAATTAACATGATTCCTTATAA
AGAAGATCCAACAAACGTTCTCGAGGTCTATGACAGAAATTTTATAAGAATGAGAATAGTTCCACTCTTTAAGGAACTCA
ACGCTTCTTTTGAAGATGCTATTTATAGGTTTTACATGAATATTATGGAAAGCTACAACATTATTGAAGAAAAGGTTCGA
AGCTTTATTTCCAGTTTGGAAACGGAAACAGAGAATGGAAAGGTGCAAAGGTGCTTTGGGGAGCTGGATATCTTTTTGAA
AGAACAAGACGCCCTTGTGGCTGAAGTGGTAAGAGAGATTGTAATGCGATTATCTGAGGATGGGTATCCACCTTCAAGGG
AAAGAATAGAAGCGTTTATGAAACTCTTAGGCAAACAGGATAGAAGATGGACCATTGAGTTCAAAGAGGATATCAAATCG
AGTCGTTTGGGGAAATATATATTTTTTTATAGAGGAGATCTGTTTGAAAGTATGAAAGACGATGTTGATGAAGTTATCGA
AAAACTGCCGGTCAGAGTCTCAACAAGGTTCGGAGAGATTATTCTGAATTATGAGAAAGGTAAATTGGAACTCAAAGAAA
AATTCGATGGTAAAAAAGTTTGTCCATGTACTCTTAAAGTATTAAAATTTCCTTTGAGGTTCCGAAGGGTAAAATGTGAA
GATAGAATGATTCCTTTCGGGAGCAAAAAAGAAAAGGAAGTTTTTAAAATCATTAAAGAAAGTGGAATGACAGGTTTTAT
TACACGGATACCTGTACTGGAGAATGCTGATGGCACAATTTTATGGGTACCGGGAATTAGAGCCGCTGAGCAGTGCAGGG
TGTCCGATTCCGAAGACGGATACGCCCTTTTTAGATTTGAAAGGAGGTAA

Upstream 100 bases:

>100_bases
CATTCGTAATGAGCAGGTTGTGGGTTCGAGTCCCACCGTTGGCTCCATGCGCCCAAACCGGGCGCTTTATTTTTAATCAA
AGTGAATGGGTGGCGAGAAA

Downstream 100 bases:

>100_bases
TTTGGTGGATCAAAGGCCTAAGTTTGGCATGATTCTATTCTATATCGTGTTGGGAGTCTTCTTAATGGTTGCTCTTAGAG
GGCTATATACTACTGACACA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTGFEKKVFEFIDRWGLIKQGEKILVAVSGGKDSMALLHFLVSKKERLGCEICVANMDHGLRDDSGREEAVMIASFCEKH
DVPFYHERRDVTQYHKNNRGLSLEEAARIVRYDFLFNAREKLGADKIALAHHLNDLVETMLMRIARGTGLKGVISMKPMN
NSIIRPLLSVGVQEILDYVTINMIPYKEDPTNVLEVYDRNFIRMRIVPLFKELNASFEDAIYRFYMNIMESYNIIEEKVR
SFISSLETETENGKVQRCFGELDIFLKEQDALVAEVVREIVMRLSEDGYPPSRERIEAFMKLLGKQDRRWTIEFKEDIKS
SRLGKYIFFYRGDLFESMKDDVDEVIEKLPVRVSTRFGEIILNYEKGKLELKEKFDGKKVCPCTLKVLKFPLRFRRVKCE
DRMIPFGSKKEKEVFKIIKESGMTGFITRIPVLENADGTILWVPGIRAAEQCRVSDSEDGYALFRFERR

Sequences:

>Translated_469_residues
MTGFEKKVFEFIDRWGLIKQGEKILVAVSGGKDSMALLHFLVSKKERLGCEICVANMDHGLRDDSGREEAVMIASFCEKH
DVPFYHERRDVTQYHKNNRGLSLEEAARIVRYDFLFNAREKLGADKIALAHHLNDLVETMLMRIARGTGLKGVISMKPMN
NSIIRPLLSVGVQEILDYVTINMIPYKEDPTNVLEVYDRNFIRMRIVPLFKELNASFEDAIYRFYMNIMESYNIIEEKVR
SFISSLETETENGKVQRCFGELDIFLKEQDALVAEVVREIVMRLSEDGYPPSRERIEAFMKLLGKQDRRWTIEFKEDIKS
SRLGKYIFFYRGDLFESMKDDVDEVIEKLPVRVSTRFGEIILNYEKGKLELKEKFDGKKVCPCTLKVLKFPLRFRRVKCE
DRMIPFGSKKEKEVFKIIKESGMTGFITRIPVLENADGTILWVPGIRAAEQCRVSDSEDGYALFRFERR
>Mature_468_residues
TGFEKKVFEFIDRWGLIKQGEKILVAVSGGKDSMALLHFLVSKKERLGCEICVANMDHGLRDDSGREEAVMIASFCEKHD
VPFYHERRDVTQYHKNNRGLSLEEAARIVRYDFLFNAREKLGADKIALAHHLNDLVETMLMRIARGTGLKGVISMKPMNN
SIIRPLLSVGVQEILDYVTINMIPYKEDPTNVLEVYDRNFIRMRIVPLFKELNASFEDAIYRFYMNIMESYNIIEEKVRS
FISSLETETENGKVQRCFGELDIFLKEQDALVAEVVREIVMRLSEDGYPPSRERIEAFMKLLGKQDRRWTIEFKEDIKSS
RLGKYIFFYRGDLFESMKDDVDEVIEKLPVRVSTRFGEIILNYEKGKLELKEKFDGKKVCPCTLKVLKFPLRFRRVKCED
RMIPFGSKKEKEVFKIIKESGMTGFITRIPVLENADGTILWVPGIRAAEQCRVSDSEDGYALFRFERR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=206, Percent_Identity=33.495145631068, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI193211090, Length=224, Percent_Identity=24.1071428571429, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54587; Mature: 54456

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFEKKVFEFIDRWGLIKQGEKILVAVSGGKDSMALLHFLVSKKERLGCEICVANMDHG
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
LRDDSGREEAVMIASFCEKHDVPFYHERRDVTQYHKNNRGLSLEEAARIVRYDFLFNARE
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHH
KLGADKIALAHHLNDLVETMLMRIARGTGLKGVISMKPMNNSIIRPLLSVGVQEILDYVT
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
INMIPYKEDPTNVLEVYDRNFIRMRIVPLFKELNASFEDAIYRFYMNIMESYNIIEEKVR
HEEEECCCCCHHHHHHHCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFISSLETETENGKVQRCFGELDIFLKEQDALVAEVVREIVMRLSEDGYPPSRERIEAFM
HHHHHHHCCCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KLLGKQDRRWTIEFKEDIKSSRLGKYIFFYRGDLFESMKDDVDEVIEKLPVRVSTRFGEI
HHHCCCCCEEEEEEHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHCCCCCEE
ILNYEKGKLELKEKFDGKKVCPCTLKVLKFPLRFRRVKCEDRMIPFGSKKEKEVFKIIKE
EEEECCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
SGMTGFITRIPVLENADGTILWVPGIRAAEQCRVSDSEDGYALFRFERR
CCCCEEEEECCEEECCCCCEEECCCCCCHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TGFEKKVFEFIDRWGLIKQGEKILVAVSGGKDSMALLHFLVSKKERLGCEICVANMDHG
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
LRDDSGREEAVMIASFCEKHDVPFYHERRDVTQYHKNNRGLSLEEAARIVRYDFLFNARE
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHH
KLGADKIALAHHLNDLVETMLMRIARGTGLKGVISMKPMNNSIIRPLLSVGVQEILDYVT
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
INMIPYKEDPTNVLEVYDRNFIRMRIVPLFKELNASFEDAIYRFYMNIMESYNIIEEKVR
HEEEECCCCCHHHHHHHCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFISSLETETENGKVQRCFGELDIFLKEQDALVAEVVREIVMRLSEDGYPPSRERIEAFM
HHHHHHHCCCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KLLGKQDRRWTIEFKEDIKSSRLGKYIFFYRGDLFESMKDDVDEVIEKLPVRVSTRFGEI
HHHCCCCCEEEEEEHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHCCCCCEE
ILNYEKGKLELKEKFDGKKVCPCTLKVLKFPLRFRRVKCEDRMIPFGSKKEKEVFKIIKE
EEEECCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
SGMTGFITRIPVLENADGTILWVPGIRAAEQCRVSDSEDGYALFRFERR
CCCCEEEEECCEEECCCCCEEECCCCCCHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA